uniprot:Q42588 [serine O-acetyltransferase(Enzyme) wildtype]
L_Serine + Acetyl_CoA -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Hepes/HCl
PH: 7.5
Temperature: 25 |
uniprot:Q42588 [serine O-acetyltransferase(Enzyme) wildtype]
Acetyl_CoA + L_Serine -> O_Acetyl_L_serine + Coenzyme_A |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Hepes/HCl
PH: 7.5
Temperature: 25 |
- [serine O-acetyltransferase(Enzyme) wildtype bound to O-Acetylserine (thiol) lyase (Escherichia coli)]
L_Serine + Acetyl_CoA -> O_Acetyl_L_serine + Coenzyme_A |
(( (( (kcat * E) ) * (S ^ h)) ) / (1 + (( (S / Km) ) ^ h))) |
|
buffer: 50 mM Hepes/HCl, 2.5 mM sulfide
PH: 7.5
Temperature: 25 |
- [serine O-acetyltransferase(Enzyme) wildtype bound to recombinant chloroplastic O-Acetylserine (thiol) lyase (Spinacia oleracea)]
L_Serine + Acetyl_CoA -> O_Acetyl_L_serine + Coenzyme_A |
(( (( (kcat * E) ) * (S ^ h)) ) / (1 + (( (S / Km) ) ^ h))) |
|
buffer: 50 mM Hepes/HCl, 2.5 mM sulfide
PH: 7.5
Temperature: 25 |
- [serine O-acetyltransferase(Enzyme) wildtype bound to recombinant chloroplastic O-Acetylserine (thiol) lyase (Spinacia oleracea)]
L_Serine + Acetyl_CoA -> O_Acetyl_L_serine + Coenzyme_A |
(( (( (kcat * E) ) * (S ^ h)) ) / (1 + (( (S / Km) ) ^ h))) |
|
buffer: 50 mM Hepes/HCl, 2.5 mM sulfide
PH: 7.5
Temperature: 25 |
- [serine O-acetyltransferase(Enzyme) wildtype bound to O-Acetylserine (thiol) lyase (Escherichia coli)]
Acetyl_CoA + L_Serine -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (kcat * E) ) * (S ^ h)) ) / (1 + (( (S / Km) ) ^ h))) |
|
buffer: 50 mM Hepes/HCl, 2.5 mM sulfide
PH: 7.5
Temperature: 25 |
- [serine O-acetyltransferase(Enzyme) wildtype]
Coenzyme_A + O_Acetyl_L_serine -> L_Serine + Acetyl_CoA |
(( (( (E * A) ) * B) ) / (( (( (( (( (Phi_0 * A) ) * B) ) + (Phi_A * B)) ) + (Phi_B * A)) ) + Phi_AB)) |
|
buffer: TSE, 5 mM L-Malate, 1.5 mM NAD+, 0.11 mM NADH, 2 units malate dehydrogenase, 4 units citrate synthase
PH: 7.5
Temperature: 25 |
- [serine O-acetyltransferase(Enzyme) wildtype]
Acetyl_CoA + L_Serine -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (E * A) ) * B) ) / (( (( (( (( (Phi_0 * A) ) * B) ) + (Phi_A * B)) ) + (Phi_B * A)) ) + Phi_AB)) |
|
buffer: TSE, 1 mM 5,5'-dithios(2-nitrobenzoic acid)
PH: 7.5
Temperature: 25 |
- [Ethyl-S-CoA]
Acetyl_CoA + L_Serine -> O_Acetyl_L_serine + Coenzyme_A |
(( (( (E * A) ) * B) ) / (( (( (( (( (Phi_0 * A) ) * B) ) + (Phi_A * B)) ) + (Phi_B * A)) ) + Phi_AB)) |
|
buffer: TSE, 1 mM 5,5'-dithios(2-nitrobenzoic acid)
PH: 7.5
Temperature: 25 |
uniprot:P43886 [H+]
O_Acetyl_L_serine + Coenzyme_A -> Acetyl_CoA + L_Serine |
(( (( (( (kcat0 / (1 + (10 ^ (pK1 - pH)))) ) * E) ) * S) ) / (( (( (kcat0 / (1 + (10 ^ (pK1 - pH)))) ) / (kcat_Km0 / (1 + (10 ^ (pK2 - pH))))) ) + S)) |
|
buffer: 100 mM phosphate
PH: 5.5
Temperature: 25 |
uniprot:P43886 [H+]
Coenzyme_A + O_Acetyl_L_serine -> L_Serine + Acetyl_CoA |
(( (( (( (kcat0 / (1 + (10 ^ (pK1 - pH)))) ) * E) ) * A) ) / (( (( (kcat0 / (1 + (10 ^ (pK1 - pH)))) ) / (kcat_Km0 / (1 + (10 ^ (pK2 - pH))))) ) + A)) |
|
buffer: 100 mM phosphate
PH: 5.5
Temperature: 25 |
uniprot:P43886 [H+]
Acetyl_CoA + L_Serine -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (( (kcat0 / (1 + (10 ^ (pK1 - pH)))) ) * E) ) * S) ) / (( (( (kcat0 / (1 + (10 ^ (pK1 - pH)))) ) / (kcat_Km0 / (1 + (10 ^ (pK2 - pH))))) ) + S)) |
|
buffer: 100 mM 2-(N-morpholino)ethanesulfonic acid monohydrate (pH 5.5 - 6.5), 100 mM bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane (pH 6.0), 100 mM HEPES (pH 6.5 - 8.5), 100 mM Tris (pH 7.5), 100 mM N-[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid (pH 8.5), 100 mM 2-(N-cyclohexylamino)ethanesulfonic acid (pH 9.0 - 10.0), 0.45 mM 5,5'-dithiobis(2-nitrobencoic acid)
PH: 5.5
Temperature: 25 |
uniprot:P43886 [H+]
L_Serine + Acetyl_CoA -> O_Acetyl_L_serine + Coenzyme_A |
(( (( (( (kcat0 / (1 + (10 ^ (pK1 - pH)))) ) * E) ) * A) ) / (( (( (kcat0 / (1 + (10 ^ (pK1 - pH)))) ) / (kcat_Km0 / (1 + (10 ^ (pK2 - pH))))) ) + A)) |
|
buffer: 100 mM 2-(N-morpholino)ethanesulfonic acid monohydrate (pH 5.5 - 6.5), 100 mM bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane (pH 6.0), 100 mM HEPES (pH 6.5 - 8.5), 100 mM Tris (pH 7.5), 100 mM N-[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid (pH 8.5), 100 mM 2-(N-cyclohexylamino)ethanesulfonic acid (pH 9.0 - 10.0), 0.45 mM 5,5'-dithiobis(2-nitrobencoic acid)
PH: 5.5
Temperature: 25 |
uniprot:P0A9D4 [L-Cysteine]
Acetyl_CoA + L_Serine -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (( (( (E * kcat) ) * k) ) * E) ) * (( (A * B) ) - (( (C * D) ) / Keq1))) ) / (( (( (( (( (( (( (( (( (( (k * E) ) * KmA) ) * B) ) * (1 + (I / Ki2I))) ) + (( (( (( (k * E) ) * KmB) ) * A) ) * (1 + (I / Ki1I)))) ) + (( (( (( (( (kcat * E) ) * KmD) ) * C) ) / Keq1) ) * (1 + (I / Ki1I)))) ) + (( (( (( (( (kcat * E) ) * KmC) ) * D) ) / Keq1) ) * (1 + (I / Ki2I)))) ) + (( (( (k * E) ) * B) ) * A)) ) + (( (( (( (( (( (kcat * E) ) * KmD) ) * B) ) * C) ) / Keq1) ) * KiB)) ) + (( (( (( (( (k * E) ) * KmB) ) * A) ) * D) ) / KiD))) |
|
buffer: 50 mM potassium phosphate, 1 mM EDTA, 0.5 mM DTNB
PH: 7.5
Temperature: 25 |
uniprot:P0A9D4 [L-Cysteine]
L_Serine + Acetyl_CoA -> O_Acetyl_L_serine + Coenzyme_A |
(( (( (( (( (E * kcat) ) * k) ) * E) ) * (( (A * B) ) - (( (C * D) ) / Keq1))) ) / (( (( (( (( (( (( (( (( (( (k * E) ) * KmA) ) * B) ) * (1 + (I / Ki2I))) ) + (( (( (( (k * E) ) * KmB) ) * A) ) * (1 + (I / Ki1I)))) ) + (( (( (( (( (kcat * E) ) * KmD) ) * C) ) / Keq1) ) * (1 + (I / Ki1I)))) ) + (( (( (( (( (kcat * E) ) * KmC) ) * D) ) / Keq1) ) * (1 + (I / Ki2I)))) ) + (( (( (k * E) ) * B) ) * A)) ) + (( (( (( (( (( (kcat * E) ) * KmD) ) * B) ) * C) ) / Keq1) ) * KiB)) ) + (( (( (( (( (k * E) ) * KmB) ) * A) ) * D) ) / KiD))) |
|
buffer: 50 mM potassium phosphate, 1 mM EDTA, 0.5 mM DTNB
PH: 7.5
Temperature: 25 |
uniprot:P0A9D4 [L-Cysteine]
Acetyl_CoA + L_Serine -> O_Acetyl_L_serine + Coenzyme_A |
(( (( (( (( (E * kcat) ) * k) ) * E) ) * (( (A * B) ) - (( (C * D) ) / Keq1))) ) / (( (( (( (( (( (( (( (( (( (k * E) ) * KmA) ) * B) ) * (1 + (I / Ki2I))) ) + (( (( (( (k * E) ) * KmB) ) * A) ) * (1 + (I / Ki1I)))) ) + (( (( (( (( (kcat * E) ) * KmD) ) * C) ) / Keq1) ) * (1 + (I / Ki1I)))) ) + (( (( (( (( (kcat * E) ) * KmC) ) * D) ) / Keq1) ) * (1 + (I / Ki2I)))) ) + (( (( (k * E) ) * B) ) * A)) ) + (( (( (( (( (( (kcat * E) ) * KmD) ) * B) ) * C) ) / Keq1) ) * KiB)) ) + (( (( (( (( (k * E) ) * KmB) ) * A) ) * D) ) / KiD))) |
|
buffer: 50 mM potassium phosphate, 1 mM EDTA, 0.5 mM DTNB, 0.1 mM Pyridoxal 5-phosphate
PH: 7.5
Temperature: 25 |
uniprot:P0A9D4 [L-Cysteine]
L_Serine + Acetyl_CoA -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (I / Ki1I))) ) + (( (KmA * B) ) * (1 + (I / Ki2I)))) ) + (B * A))) |
|
buffer: 50 mM potassium phosphate, 1 mM EDTA, 0.5 mM DTNB, 0.1 mM Pyridoxal 5-phosphate
PH: 7.5
Temperature: 25 |
uniprot:P0A9D4 [L-Cysteine]
L_Serine + Acetyl_CoA -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (I / Ki1I))) ) + (( (KmA * B) ) * (1 + (I / Ki2I)))) ) + (B * A))) |
|
buffer: 50 mM potassium phosphate, 1 mM EDTA, 0.5 mM DTNB
PH: 7.5
Temperature: 25 |
uniprot:P0A9D4 [L-Cysteine]
L_Serine + Acetyl_CoA -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (I / Ki1I))) ) + (( (KmA * B) ) * (1 + (I / Ki2I)))) ) + (B * A))) |
|
buffer: 50 mM potassium phosphate, 1 mM EDTA, 0.5 mM DTNB
PH: 7.5
Temperature: 25 |
uniprot:P0A9D4 [serine O-acetyltransferase(Enzyme) wildtype]
Coenzyme_A + O_Acetyl_L_serine -> Acetyl_CoA + L_Serine |
(( (( (( (E * kcat) ) * D) ) * C) ) / (( (( (KmC * D) ) + (KmD * C)) ) + (D * C))) |
|
buffer: 50 mM potassium phosphate, 1 mM EDTA, 0.5 mM DTNB
PH: 7.5
Temperature: 25 |
uniprot:P0A9D4 [serine O-acetyltransferase(Enzyme) mutant 20 amino acids deleted at C-terminus]
O_Acetyl_L_serine + Coenzyme_A -> L_Serine + Acetyl_CoA |
(( (( (( (E * kcat) ) * D) ) * C) ) / (( (( (KmC * D) ) + (KmD * C)) ) + (D * C))) |
|
buffer: 50 mM potassium phosphate, 1 mM EDTA, 0.5 mM DTNB
PH: 7.5
Temperature: 25 |
- [serine O-acetyltransferase(Enzyme) wildtype]
L_Serine + Propanoyl_CoA -> Coenzyme_A + O_Propionyl_L_serine |
(( (( (E * A) ) * B) ) / (( (( (( (( (Phi_0 * A) ) * B) ) + (Phi_A * B)) ) + (Phi_B * A)) ) + Phi_AB)) |
|
buffer: 50 mM Tris/HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM 5,5-dithiobis(2-nitrobenzoicacid)
PH: 7.5
Temperature: 25 |
- [serine O-acetyltransferase(Enzyme) wildtype]
L_Threonine + Acetyl_CoA -> O_Acetyl_L_threonine + Coenzyme_A |
(( (( (E * A) ) * B) ) / (( (( (( (( (Phi_0 * A) ) * B) ) + (Phi_A * B)) ) + (Phi_B * A)) ) + Phi_AB)) |
|
buffer: 50 mM Tris/HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM 5,5-dithiobis(2-nitrobenzoicacid)
PH: 7.5
Temperature: 25 |
- [serine O-acetyltransferase(Enzyme) wildtype]
Acetyl_CoA + L_Serine -> Coenzyme_A + O_Acetyl_L_serine |
(( (V * substrate) ) / (Km + substrate)) |
|
buffer: 50 mM Tris-HCl, 1 mM sodium-EDTA, 1 mM 5,5'-dithiobis(2-nitrobenzoic acid)
PH: 7.6
Temperature: 25 |
- [serine O-acetyltransferase(Enzyme) wildtype]
L_Serine + Acetyl_CoA -> O_Acetyl_L_serine + Coenzyme_A |
(( (V * substrate) ) / (Km + substrate)) |
|
buffer: 50 mM Tris-HCl, 1 mM sodium-EDTA, 1 mM 5,5'-dithiobis(2-nitrobenzoic acid)
PH: 7.6
Temperature: 25 |
uniprot:Q9BKB1 [serine O-acetyltransferase(Enzyme) wildtype fusion rGST-TcSAT]
L_Serine + Acetyl_CoA -> Coenzyme_A + O_Acetyl_L_serine |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl
PH: 8
Temperature: 25 |
uniprot:Q9BKB1 [serine O-acetyltransferase(Enzyme) wildtype fusion rGST-TcSAT]
Acetyl_CoA + L_Serine -> O_Acetyl_L_serine + Coenzyme_A |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl
PH: 8
Temperature: 25 |
uniprot:P43886 [desulfo-CoA]
Coenzyme_A + O_Acetyl_L_serine -> L_Serine + Acetyl_CoA |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [Cysteine]
O_Acetyl_L_serine + Coenzyme_A -> L_Serine + Acetyl_CoA |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [Cysteine]
Coenzyme_A + O_Acetyl_L_serine -> L_Serine + Acetyl_CoA |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [Glycine]
Acetyl_CoA + L_Serine -> O_Acetyl_L_serine + Coenzyme_A |
(( (Vmax * A) ) / (Ka + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 0.45 mM DTNB
PH: 7.5
Temperature: 25 |
uniprot:P43886 [Glycine]
Acetyl_CoA + L_Serine -> O_Acetyl_L_serine + Coenzyme_A |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Tris-HCl, 0.45 mM DTNB
PH: 7.5
Temperature: 25 |
uniprot:P43886 [desulfo-CoA]
Coenzyme_A + O_Acetyl_L_serine -> L_Serine + Acetyl_CoA |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [S-Methyl-L-cysteine]
O_Acetyl_L_serine + Coenzyme_A -> Acetyl_CoA + L_Serine |
(( (Vmax * A) ) / (Ka + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [S-Methyl-L-cysteine]
Coenzyme_A + O_Acetyl_L_serine -> Acetyl_CoA + L_Serine |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [serine O-acetyltransferase(Enzyme) wildtype]
O_Acetyl_L_serine + Coenzyme_A -> L_Serine + Acetyl_CoA |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [Cysteine]
L_Serine + Acetyl_CoA -> Coenzyme_A + O_Acetyl_L_serine |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [Glycine]
Acetyl_CoA + L_Serine -> O_Acetyl_L_serine + Coenzyme_A |
(( (Vmax * A) ) / (Ka + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Mes, 0.45 mM DTNB
PH: 6.5
Temperature: 25 |
uniprot:P43886 [Glycine]
Acetyl_CoA + L_Serine -> Coenzyme_A + O_Acetyl_L_serine |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Mes, 0.45 mM DTNB
PH: 6.5
Temperature: 25 |
uniprot:P43886 [Cysteine]
Acetyl_CoA + L_Serine -> O_Acetyl_L_serine + Coenzyme_A |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [desulfo-CoA]
L_Serine + Acetyl_CoA -> O_Acetyl_L_serine + Coenzyme_A |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Mes, 10 mM glycine, 0.45 mM DTNB
PH: 6.5
Temperature: 25 |
uniprot:P43886 [desulfo-CoA]
L_Serine + Acetyl_CoA -> O_Acetyl_L_serine + Coenzyme_A |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Mes, 10 mM glycine, 0.45 mM DTNB
PH: 6.5
Temperature: 25 |
uniprot:P43886 [serine O-acetyltransferase(Enzyme) wildtype]
Acetyl_CoA + L_Serine -> O_Acetyl_L_serine + Coenzyme_A |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Mes, 10 mM glycine, 0.45 mM DTNB
PH: 6.5
Temperature: 25 |
uniprot:P43886 [serine O-acetyltransferase(Enzyme) wildtype]
Coenzyme_A + O_Acetyl_L_serine -> Acetyl_CoA + L_Serine |
(( (Vmax * A) ) / (( (Ka * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [serine O-acetyltransferase(Enzyme) wildtype]
L_Serine + Acetyl_CoA -> Coenzyme_A + O_Acetyl_L_serine |
(( (Vmax * A) ) / (Ka + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Mes, 10 mM glycine, 0.45 mM DTNB
PH: 6.5
Temperature: 25 |
uniprot:P43886 [serine O-acetyltransferase(Enzyme) wildtype]
O_Acetyl_L_serine + Coenzyme_A -> Acetyl_CoA + L_Serine |
(( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Ka * B)) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [serine O-acetyltransferase(Enzyme) wildtype]
L_Serine + Acetyl_CoA -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (Kia * Kb) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 100 mM phosphate, 10 mM glycine
PH: 6.5
Temperature: 25 |
uniprot:P43886 [serine O-acetyltransferase(Enzyme) wildtype]
L_Serine + Acetyl_CoA -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (Kia * Kb) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 100 mM Mes, 10 mM glycine, 0.45 mM DTNB
PH: 6.5
Temperature: 25 |
uniprot:P43886 [serine O-acetyltransferase(Enzyme) wildtype]
Acetyl_CoA + L_Serine -> Coenzyme_A + O_Acetyl_L_serine |
(( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (Kia * Kb) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 100 mM Tris-HCl, 10 mM glycine, 0.45 mM DTNB
PH: 7.5
Temperature: 25 |