EC: 3.1.1.1

carboxylesterase (carboxylic-ester hydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q8I034 [carboxylesterase(Enzyme) wildtype cauxin]

H2O + p_Nitrophenyl_acetate -> p_Nitrophenol + Acetate
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl
PH: 8
Temperature: 36
uniprot:P13001 [carboxylesterase(Enzyme) wildtype BioH]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40.5 mM Tris-HCl, 0.0036 g/ml Triton X-100, 0.0009 g/ml Gum arabic
PH: 8
Temperature: 37
uniprot:P13001 [carboxylesterase(Enzyme) wildtype BioH]

H2O + p_Nitrophenyl_caproate -> Hexanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40.5 mM Tris-HCl, 0.0036 g/ml Triton X-100, 0.0009 g/ml Gum arabic
PH: 8
Temperature: 37
uniprot:P13001 [carboxylesterase(Enzyme) wildtype BioH]

p_Nitrophenyl_laurate + H2O -> Dodecanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40.5 mM Tris-HCl, 0.0036 g/ml Triton X-100, 0.0009 g/ml Gum arabic
PH: 8
Temperature: 37
uniprot:P13001 [carboxylesterase(Enzyme) wildtype BioH]

H2O + p_Nitrophenyl_acetate -> Acetate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40.5 mM Tris-HCl, 0.0036 g/ml Triton X-100, 0.0009 g/ml Gum arabic
PH: 8
Temperature: 37
uniprot:P13001 [carboxylesterase(Enzyme) wildtype BioH]

H2O + p_Nitrophenyl_propionate -> Propanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40.5 mM Tris-HCl, 0.0036 g/ml Triton X-100, 0.0009 g/ml Gum arabic
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

Irinotecan + H2O -> SN_38 + 1,4'_Bipiperidine_1'_carboxylic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate
PH: 7.4
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

Irinotecan + H2O -> SN_38 + 1,4'_Bipiperidine_1'_carboxylic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate
PH: 7.4
Temperature: 37
uniprot:Q6LAP9 [carboxylesterase(Enzyme) wildtype]

Irinotecan + H2O -> SN_38 + 1,4'_Bipiperidine_1'_carboxylic_acid
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes, 10% ethylene glycol
PH: 7
Temperature: 37
uniprot:Q4G0E9 [carboxylesterase(Enzyme) wildtype]

H2O + Irinotecan -> 1,4'_Bipiperidine_1'_carboxylic_acid + SN_38
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes, 10% ethylene glycol
PH: 7
Temperature: 37
uniprot:Q6UWW8 [carboxylesterase(Enzyme) wildtype]

Irinotecan + H2O -> SN_38 + 1,4'_Bipiperidine_1'_carboxylic_acid
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes, 10% ethylene glycol
PH: 7
Temperature: 37
uniprot:Q4G0E9 [carboxylesterase(Enzyme) wildtype]

H2O + 7_Ethyl_10_[4_N_(5_aminopentanoic_acid)_1_piperidino]_carbonyloxycamptothecin -> SN_38 + 4_((4_Carboxybutyl)amino)piperidine_1_carboxylic_acid
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes, 10% ethylene glycol
PH: 7
Temperature: 37
uniprot:Q6UWW8 [carboxylesterase(Enzyme) wildtype]

H2O + 7_Ethyl_10_[4_(1_piperidino)_1_amino]_carbonyloxycamptothecin -> 4_Amino_1_piperidinecarboxylic_acid + SN_38
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes, 10% ethylene glycol
PH: 7
Temperature: 37
uniprot:Q4G0E9 [carboxylesterase(Enzyme) wildtype]

H2O + 7_Ethyl_10_[4_(1_piperidino)_1_amino]_carbonyloxycamptothecin -> SN_38 + 4_Amino_1_piperidinecarboxylic_acid
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes, 10% ethylene glycol
PH: 7
Temperature: 37
uniprot:Q6LAP9 [carboxylesterase(Enzyme) wildtype]

H2O + 7_Ethyl_10_[4_(1_piperidino)_1_amino]_carbonyloxycamptothecin -> SN_38 + 4_Amino_1_piperidinecarboxylic_acid
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes, 10% ethylene glycol
PH: 7
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme CES2]

Capecitabine + H2O -> 5'_Deoxy_5_fluorocytidine + Unknown
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 10% Ethylene glycol
PH: 7.4
Temperature: 37
uniprot:Q6LAP9 [carboxylesterase(Enzyme) wildtype isoenzyme CES1A1]

Capecitabine + H2O -> 5'_Deoxy_5_fluorocytidine + Unknown
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 10% Ethylene glycol
PH: 7.4
Temperature: 37
uniprot:Q06174 [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_caproate -> p_Nitrophenol + Hexanoate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 100 µg/ml bovine serum albumin.
PH: 8.5
Temperature: 60
uniprot:Q8GCC7 [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_caproate + H2O -> Hexanoate + p_Nitrophenol
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 100 µg/ml bovine serum albumin.
PH: 8.5
Temperature: 60
- [Taurocholate]

p_Nitrophenyl_acetate + H2O -> p_Nitrophenol + Acetate
(( (Vmax * S) ) / (( (( (Ks * (1 + (A / Ka))) ) / (1 + (( (beta * A) ) / (alpha * Ka)))) ) + (( (S * (1 + (A / (alpha * Ka)))) ) / (1 + (( (beta * A) ) / (alpha * Ka)))))) buffer: 100 mM Sodium phosphate
PH: 7.5
Temperature: 25
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + 1_Naphthylacetic_acid -> alpha_Naphthol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

1_Naphthylacetic_acid + H2O -> Acetate + alpha_Naphthol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + 1_Naphthylacetic_acid -> Acetate + alpha_Naphthol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

1_Naphthylacetic_acid + H2O -> alpha_Naphthol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + 1_Naphthylacetic_acid -> Acetate + alpha_Naphthol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + 1_Naphthylacetic_acid -> Acetate + alpha_Naphthol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

1_Naphthylacetic_acid + H2O -> Acetate + alpha_Naphthol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

1_Naphthylacetic_acid + H2O -> alpha_Naphthol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + 1_Naphthylacetic_acid -> alpha_Naphthol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

1_Naphthylacetic_acid + H2O -> alpha_Naphthol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

1_Naphthylacetic_acid + H2O -> Acetate + alpha_Naphthol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + 1_Naphthylacetic_acid -> Acetate + alpha_Naphthol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_acetate -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_octanoate + H2O -> Octanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer:
PH: 7.5
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer:
PH: 7.5
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_caproate + H2O -> Hexanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer:
PH: 7.5
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer:
PH: 7.5
Temperature: 37
uniprot:P16303 [carboxylesterase(Enzyme) wildtype ES10]

H2O + All_trans_Retinyl_palmitate -> Retinol + Palmitate
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium acetate, 0.05 % Triton X-100
PH: 5
Temperature: 37
uniprot:Q7M0L6 [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 70
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 70
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 25
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_dodecanoate + H2O -> Dodecanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 30
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant R215L]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 70
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S]

H2O + p_Nitrophenyl_dodecanoate -> Dodecanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 70
uniprot:Q7M0L6 [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 25
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_dodecanoate + H2O -> Dodecanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 72.5
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_dodecanoate + H2O -> Dodecanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 55
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 35
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_dodecanoate + H2O -> Dodecanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 45
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_dodecanoate + H2O -> Dodecanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 75
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S/R215L]

H2O + p_Nitrophenyl_dodecanoate -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 77.5
uniprot:Q7M0L6 [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 30
uniprot:Q7M0L6 [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 65
uniprot:Q7M0L6 [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 72.5
uniprot:Q7M0L6 [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 45
uniprot:Q7M0L6 [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_dodecanoate -> Dodecanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 77.5
uniprot:Q7M0L6 [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 35
uniprot:Q7M0L6 [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_dodecanoate -> p_Nitrophenol + Dodecanoate
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 65
uniprot:Q7M0L6 [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_dodecanoate + H2O -> Dodecanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 55
uniprot:Q7M0L6 [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_dodecanoate + H2O -> Dodecanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 40 mM phosphate, 4 % (v/v) acetonitrile
PH: 7.1
Temperature: 75
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_butyrate + H2O -> Butanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 30 mM Sodium pyrophosphate
PH: 7
Temperature: 22
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 30 mM Sodium pyrophosphate
PH: 7
Temperature: 22
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_propionate -> Propanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 30 mM Sodium pyrophosphate
PH: 7
Temperature: 22
- [Chloral hydrate]

H2O + p_Nitrophenyl_acetate -> Acetate + p_Nitrophenol
(( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + S)) buffer: 30 mM Sodium pyrophosphate
PH: 7
Temperature: 22
uniprot:Q8I034 [carboxylesterase(Enzyme) wildtype]

3_Methylbutanol_cysteinylglycine + H2O -> Glycine + Felinine
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.2
Temperature: 38
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme CES1A1]

L_Methylphenidate + H2O -> Ritalinic_acid + Methanol
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 10% (v/v) Ethylene glycol
PH: 7
Temperature: 25
uniprot:P23141 [carboxylesterase(Enzyme) wildtype]

H2O + Oseltamivir -> Unknown + Oseltamivir_carboxylate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype]

Oseltamivir + H2O -> Oseltamivir_carboxylate + Unknown
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme CES1A1]

D_Methylphenidate + H2O -> Methanol + Ritalinic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 10% (v/v) Ethylene glycol
PH: 7
Temperature: 25
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme CES1A1]

H2O + D_Methylphenidate -> Methanol + Ritalinic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 10% (v/v) Ethylene glycol
PH: 7
Temperature: 25
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme CES1A1]

H2O + D_Methylphenidate -> Ritalinic_acid + Methanol
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 10% (v/v) Ethylene glycol
PH: 7
Temperature: 25
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme CES1A1]

H2O + D_Methylphenidate -> Methanol + Ritalinic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 10% (v/v) Ethylene glycol
PH: 7
Temperature: 25
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme CES1A1]

L_Methylphenidate + H2O -> Ritalinic_acid + Methanol
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 10% (v/v) Ethylene glycol
PH: 7
Temperature: 25
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme CES1A1]

L_Methylphenidate + H2O -> Methanol + Ritalinic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 10% (v/v) Ethylene glycol
PH: 7
Temperature: 25
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme CES1A1]

L_Methylphenidate + H2O -> Methanol + Ritalinic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 10% (v/v) Ethylene glycol
PH: 7
Temperature: 25
uniprot:P23141 [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 10 mM HEPES
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) mutant p.Gly143Glu]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 10 mM HEPES
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) mutant p.Asp260fs]

p_Nitrophenyl_acetate + H2O -> p_Nitrophenol + Acetate
(( (Vmax * A) ) / (Km + A)) buffer: 10 mM HEPES
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype]

D_Methylphenidate + H2O -> Methanol + Ritalinic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 10 mM HEPES
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype]

L_Methylphenidate + H2O -> Methanol + Ritalinic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 10 mM HEPES
PH: 7.4
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoform hCE-2]

H2O + Propyl_4_aminobenzoate -> 4_Aminobenzoate + 1_Propanol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoform hCE-1]

Propyl_4_aminobenzoate + H2O -> 1_Propanol + 4_Aminobenzoate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.4
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoform hCE-2]

H2O + Benzocaine -> Ethanol + 4_Aminobenzoate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoform hCE-1]

Benzocaine + H2O -> 4_Aminobenzoate + Ethanol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.4
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoform hCE-2]

Butyl_4_aminobenzoate + H2O -> Butanol + 4_Aminobenzoate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoform hCE-1]

H2O + Butyl_4_aminobenzoate -> 4_Aminobenzoate + Butanol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + Nafamostat -> p_Guanidinobenzoic_acid + 6_Amidino_2_naphthol
(( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) mutant M211S]

p_Nitrophenyl_octanoate + H2O -> p_Nitrophenol + Octanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_octanoate + H2O -> p_Nitrophenol + Octanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant R215L]

H2O + p_Nitrophenyl_octanoate -> Octanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211T]

p_Nitrophenyl_octanoate + H2O -> Octanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_octanoate -> p_Nitrophenol + Octanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211V]

p_Nitrophenyl_butyrate + H2O -> Butanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211T]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211T]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_caproate -> Hexanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S/R215L]

H2O + p_Nitrophenyl_caproate -> p_Nitrophenol + Hexanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant R215L]

p_Nitrophenyl_caproate + H2O -> Hexanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211T]

p_Nitrophenyl_caproate + H2O -> Hexanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211V]

p_Nitrophenyl_caproate + H2O -> p_Nitrophenol + Hexanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S]

p_Nitrophenyl_caproate + H2O -> Hexanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S]

H2O + p_Nitrophenyl_butyrate -> p_Nitrophenol + Butanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S/R215L]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_butyrate + H2O -> p_Nitrophenol + Butanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant R215L]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S/R215L]

H2O + p_Nitrophenyl_hexadecanoate -> Palmitate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211T]

p_Nitrophenyl_hexadecanoate + H2O -> p_Nitrophenol + Palmitate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211V]

p_Nitrophenyl_hexadecanoate + H2O -> p_Nitrophenol + Palmitate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211V]

H2O + p_Nitrophenyl_tetradecanoate -> p_Nitrophenol + Myristic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant R215L]

p_Nitrophenyl_tetradecanoate + H2O -> p_Nitrophenol + Myristic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_tetradecanoate + H2O -> p_Nitrophenol + Myristic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S]

p_Nitrophenyl_tetradecanoate + H2O -> Myristic_acid + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) wildtype]

H2O + p_Nitrophenyl_tetradecanoate -> Myristic_acid + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211T]

p_Nitrophenyl_tetradecanoate + H2O -> Myristic_acid + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S]

H2O + p_Nitrophenyl_hexadecanoate -> Palmitate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant R215L]

p_Nitrophenyl_hexadecanoate + H2O -> p_Nitrophenol + Palmitate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_hexadecanoate + H2O -> Palmitate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211V]

H2O + p_Nitrophenyl_dodecanoate -> p_Nitrophenol + Dodecanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211T]

H2O + p_Nitrophenyl_dodecanoate -> p_Nitrophenol + Dodecanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_dodecanoate + H2O -> Dodecanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S]

p_Nitrophenyl_dodecanoate + H2O -> Dodecanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant R215L]

p_Nitrophenyl_dodecanoate + H2O -> p_Nitrophenol + Dodecanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211T]

p_Nitrophenyl_decanoate + H2O -> Decanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S/R215L]

p_Nitrophenyl_decanoate + H2O -> p_Nitrophenol + Decanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211V]

H2O + p_Nitrophenyl_decanoate -> Decanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211S]

p_Nitrophenyl_decanoate + H2O -> Decanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) wildtype]

p_Nitrophenyl_decanoate + H2O -> Decanoate + p_Nitrophenol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant R215L]

p_Nitrophenyl_decanoate + H2O -> p_Nitrophenol + Decanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
- [carboxylesterase(Enzyme) mutant M211V]

p_Nitrophenyl_octanoate + H2O -> p_Nitrophenol + Octanoate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Na2HPO4/NaH2PO4, 0.09% (w/v) gum arabic, 7.5% propan-2-ol
PH: 7.1
Temperature: 70
uniprot:Q6DV84 [carboxylesterase(Enzyme) wildtype EstB2]

p_Nitrophenyl_butyrate + H2O -> Butanoate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate
PH: 7.5
Temperature: 36
uniprot:Q6DV85 [carboxylesterase(Enzyme) wildtype EstB1]

p_Nitrophenyl_butyrate + H2O -> Butanoate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate
PH: 7.5
Temperature: 36
uniprot:Q6DV85 [carboxylesterase(Enzyme) wildtype EstB1]

H2O + p_Nitrophenyl_acetate -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate
PH: 7.5
Temperature: 36
uniprot:Q6DV84 [carboxylesterase(Enzyme) wildtype EstB2]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate
PH: 7.5
Temperature: 36
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

H2O + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1R)_cis_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (1R)_cis_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

(R)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1R)_trans_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + H2O -> (1R)_trans_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

H2O + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1R)_cis_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (1R)_cis_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

p_Nitrophenyl_acetate + H2O -> p_Nitrophenol + Acetate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

H2O + alpha_Cyano_(6_methoxy_2_naphthyl)_methyl_2,2,3,3_tetramethylcyclopropane_1_carboxylate -> 1_Carboxy_2,2,3,3_tetramethylcyclopropane + alpha_Cyano_(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

H2O + alpha_Cyano_(6_methoxy_2_naphthyl)_methyl_2,2,3,3_tetramethylcyclopropane_1_carboxylate -> alpha_Cyano_(6_methoxy_2_naphthyl)_methanol + 1_Carboxy_2,2,3,3_tetramethylcyclopropane
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

alpha_Cyano_(6_methoxy_2_naphthyl)_methyl_3_(2,2_dimethylvinyl)_2,2_dimethylcyclopropane_carboxylate + H2O -> 3_(2,2_Dimethylvinyl)_2,2_dimethylcyclopropane_carboxylate + alpha_Cyano_(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

H2O + alpha_Cyano_(6_methoxy_2_naphthyl)_methyl_3_(2,2_dimethylvinyl)_2,2_dimethylcyclopropane_carboxylate -> alpha_Cyano_(6_methoxy_2_naphthyl)_methanol + 3_(2,2_Dimethylvinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

H2O + Cypermethrin -> 3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + alpha_Cyano(3_phenoxyphenyl)methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

Cypermethrin + H2O -> alpha_Cyano(3_phenoxyphenyl)methanol + 3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

alpha_Cypermethrin + H2O -> cis_Dichlorochrysanthemic_acid + alpha_R,S_Cyano_(3_phenoxyphenyl)methyl_alcohol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + alpha_Cypermethrin -> alpha_R,S_Cyano_(3_phenoxyphenyl)methyl_alcohol + cis_Dichlorochrysanthemic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

alpha_Cypermethrin + H2O -> alpha_R,S_Cyano_(3_phenoxyphenyl)methyl_alcohol + cis_Dichlorochrysanthemic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:P12337 [carboxylesterase(Enzyme) wildtype]

H2O + 1_RS_trans_Permethrin -> 3_Phenoxybenzyl_alcohol + 1_R,S_trans_Dichlorochrysanthemic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

H2O + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1R)_trans_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (1R)_trans_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

H2O + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1R)_cis_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (1R)_cis_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

(S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1R)_trans_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + H2O -> (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (1R)_trans_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

(S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1R)_cis_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + H2O -> (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (1R)_cis_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

H2O + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1S)_trans_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (1S)_trans_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

(R)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1S)_trans_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + H2O -> (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (1S)_trans_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

(R)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1S)_cis_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + H2O -> (1S)_cis_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

H2O + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1R)_trans_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (1R)_trans_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

H2O + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1S)_cis_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (1S)_cis_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + trans_Permethrin -> trans_Dichlorochrysanthemic_acid + 3_Phenoxybenzyl_alcohol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + trans_Permethrin -> 3_Phenoxybenzyl_alcohol + trans_Dichlorochrysanthemic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

trans_Permethrin + H2O -> 3_Phenoxybenzyl_alcohol + trans_Dichlorochrysanthemic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + cis_Permethrin -> cis_Dichlorochrysanthemic_acid + 3_Phenoxybenzyl_alcohol
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + cis_Permethrin -> 3_Phenoxybenzyl_alcohol + cis_Dichlorochrysanthemic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

cis_Permethrin + H2O -> 3_Phenoxybenzyl_alcohol + cis_Dichlorochrysanthemic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

Resmethrin + H2O -> 5_Benzyl_3_furylmethyl_alcohol + Methylchrysanthemic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + Resmethrin -> 5_Benzyl_3_furylmethyl_alcohol + Methylchrysanthemic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + Resmethrin -> 5_Benzyl_3_furylmethyl_alcohol + Methylchrysanthemic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

H2O + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1S)_trans_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (1S)_trans_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

H2O + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1S)_trans_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (1S)_trans_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

H2O + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1S)_cis_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (1S)_cis_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

H2O + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(1S)_cis_3_(2,2_dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate -> (1S)_cis_3_(2,2_Dichlorovinyl)_2,2_dimethylcyclopropane_carboxylate + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

(S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(2R)_2_(4_chlorophenyl)_3_methyl_butanoate + H2O -> (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (2R)_2_(4_Chlorophenyl)_3_methyl_butanoate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

(S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(2S)_2_(4_chlorophenyl)_3_methyl_butanoate + H2O -> (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol + (2S)_2_(4_Chlorophenyl)_3_methyl_butanoate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

H2O + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(2R)_2_(4_chlorophenyl)_3_methyl_butanoate -> (2R)_2_(4_Chlorophenyl)_3_methyl_butanoate + (S)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-2]

H2O + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(2R)_2_(4_chlorophenyl)_3_methyl_butanoate -> (2R)_2_(4_Chlorophenyl)_3_methyl_butanoate + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype isoenzyme hCE-1]

H2O + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methyl_(2R)_2_(4_chlorophenyl)_3_methyl_butanoate -> (2R)_2_(4_Chlorophenyl)_3_methyl_butanoate + (R)_alpha_Cyano(6_methoxy_2_naphthyl)_methanol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype hCE-1]

H2O + Resmethrin -> 5_Benzyl_3_furylmethyl_alcohol + Methylchrysanthemic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype hCE-1]

H2O + 1_RS_trans_Permethrin -> 3_Phenoxybenzyl_alcohol + 1_R,S_trans_Dichlorochrysanthemic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

H2O + trans_Permethrin -> 3_Phenoxybenzyl_alcohol + trans_Dichlorochrysanthemic_acid
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype hydrolase A]

H2O + trans_Permethrin -> trans_Dichlorochrysanthemic_acid + 3_Phenoxybenzyl_alcohol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype hydrolase B]

H2O + trans_Permethrin -> 3_Phenoxybenzyl_alcohol + trans_Dichlorochrysanthemic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype hydrolase B]

cis_Permethrin + H2O -> 3_Phenoxybenzyl_alcohol + cis_Dichlorochrysanthemic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype hydrolase A]

cis_Permethrin + H2O -> cis_Dichlorochrysanthemic_acid + 3_Phenoxybenzyl_alcohol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:P12337 [carboxylesterase(Enzyme) wildtype]

Resmethrin + H2O -> 5_Benzyl_3_furylmethyl_alcohol + Methylchrysanthemic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype hCE-2]

1_RS_trans_Permethrin + H2O -> 1_R,S_trans_Dichlorochrysanthemic_acid + 3_Phenoxybenzyl_alcohol
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:P23141 [carboxylesterase(Enzyme) wildtype hCE-1]

1_R,S_cis_Permethrin + H2O -> 3_Phenoxybenzyl_alcohol + 1_R,S_cis_Dichlorochrysanthemic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:O00748 [carboxylesterase(Enzyme) wildtype hCE-2]

H2O + 1_R,S_cis_Permethrin -> 3_Phenoxybenzyl_alcohol + 1_R,S_cis_Dichlorochrysanthemic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:P12337 [carboxylesterase(Enzyme) wildtype]

H2O + 1_R,S_cis_Permethrin -> 3_Phenoxybenzyl_alcohol + 1_R,S_cis_Dichlorochrysanthemic_acid
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
- [carboxylesterase(Enzyme) wildtype]

Deltamethrin + H2O -> 3_Phenoxybenzaldehyde + cis_Dibromochrysanthemic_acid + Cyanide
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 37
uniprot:Q9SG92 [carboxylesterase(Enzyme) wildtype His-tag]

H2O + Methyl_indole_3_acetate -> Indole_3_acetate + Methanol
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Bis-tris propane
PH: 7.5
Temperature: 22
uniprot:Q8DF91 [carboxylesterase(Enzyme) wildtype His-tagged]

p_Nitrophenyl_acetate + H2O -> Acetate + p_Nitrophenol
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM PBS
PH: 7.5
Temperature: 25
- [carboxylesterase(Enzyme) mutant chimer EstA-EstP]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.33% gum arabic, 1% dioxane
PH: 7.2
Temperature: 36
- [carboxylesterase(Enzyme) mutant chimer EstP-EstA]

H2O + p_Nitrophenyl_butyrate -> p_Nitrophenol + Butanoate
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.33% gum arabic, 1% dioxane
PH: 7.2
Temperature: 36
uniprot:O33407 [carboxylesterase(Enzyme) wildtype EstA]

p_Nitrophenyl_butyrate + H2O -> Butanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.33% gum arabic, 1% dioxane
PH: 7.2
Temperature: 36
uniprot:Q9HZY8 [carboxylesterase(Enzyme) wildtype TesA N-terminal His6-tag]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.33% gum arabic, 1% dioxane
PH: 7.2
Temperature: 36
uniprot:Q88QS0 [carboxylesterase(Enzyme) wildtype EstP]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.33% gum arabic, 1% dioxane
PH: 7.2
Temperature: 36
uniprot:Q88QS0 [carboxylesterase(Enzyme) wildtype EstP N-terminal domain (aa 1-296)]

p_Nitrophenyl_butyrate + H2O -> Butanoate + p_Nitrophenol
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.33% gum arabic, 1% dioxane
PH: 7.2
Temperature: 36

Pathways

pathway id name
BioCyc:META_PWY-8001 felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis
BioCyc:META_PWY-6857 retinol biosynthesis
BioCyc:HUMAN_PWY-6857 retinol biosynthesis
Reactome:R-HSA-9749641 Aspirin ADME
Reactome:R-HSA-9748784 Drug ADME
Reactome:R-HSA-8964043 Plasma lipoprotein clearance
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-HSA-5578768 Physiological factors
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-MMU-5578768 Physiological factors
Reactome:R-MMU-2980736 Peptide hormone metabolism
Reactome:R-HSA-8964038 LDL clearance
Reactome:R-HSA-2980736 Peptide hormone metabolism
Reactome:R-MMU-2022377 Metabolism of Angiotensinogen to Angiotensins
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-2980736 Peptide hormone metabolism
Reactome:R-RNO-397014 Muscle contraction
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-5576891 Cardiac conduction
Reactome:R-MMU-5576891 Cardiac conduction
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-RNO-5578768 Physiological factors
Reactome:R-HSA-382551 Transport of small molecules
Reactome:R-HSA-174824 Lipoprotein metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-397014 Muscle contraction
Reactome:R-HSA-2022377 Metabolism of Angiotensinogen to Angiotensins
Reactome:R-MMU-397014 Muscle contraction
Reactome:R-RNO-2022377 Metabolism of Angiotensinogen to Angiotensins
Reactome:R-RNO-5576891 Cardiac conduction
PathBank:SMP0000469 Capecitabine Action Pathway
PathBank:SMP0000600 Irinotecan Metabolism Pathway
PathBank:SMP0000652 Mycophenolic Acid Metabolism Pathway
PathBank:SMP0000433 Irinotecan Action Pathway
PathBank:SMP0000607 Capecitabine Metabolism Pathway
PathBank:SMP0000623 Heroin Metabolism Pathway