EC: 2.7.1.24

dephospho-CoA kinase (ATP:3'-dephospho-CoA 3'-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q13057 [dephospho-CoA kinase(Enzyme) wildtype]

ATP + Dephospho_CoA -> ADP + Coenzyme_A		 (( (( (E * kcat) ) * S1) ) / (Km + S1)) buffer: 50 mM Tris, 2 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 U/ml lactate dehydrogenase, 2.5 U/ml pyruvate kinase
PH: 8
Temperature: 37
uniprot:Q13057 [dephospho-CoA kinase(Enzyme) wildtype]

Dephospho_CoA + ATP -> Coenzyme_A + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris, 2 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 U/ml lactate dehydrogenase, 2.5 U/ml pyruvate kinase
PH: 8
Temperature: 37
- [dephospho-CoA kinase(Enzyme) wildtype CoaE]

ATP + Dephospho_CoA -> Coenzyme_A + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.3 mM NADH, 0.5 mM Phosphoenolpyruvate, 10 mM MgCl2, 20 mM KCl, 0.01 units/µl Pyruvate kinase, 0.01 units/µl Lactic dehydrogenase
PH: 7.6
Temperature: 25
- [dephospho-CoA kinase(Enzyme) wildtype CoaE]

3'_Dephospho_ethyldethia_CoA + ATP -> ADP + Ethyldethia_coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.3 mM NADH, 0.5 mM Phosphoenolpyruvate, 10 mM MgCl2, 20 mM KCl, 0.01 units/µl Pyruvate kinase, 0.01 units/µl Lactic dehydrogenase
PH: 7.6
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-7851 coenzyme A biosynthesis II (eukaryotic)
BioCyc:ECO_COA-PWY coenzyme A biosynthesis I (prokaryotic)
BioCyc:AGRO_COA-PWY coenzyme A biosynthesis I (prokaryotic)
BioCyc:SYNEL_COA-PWY coenzyme A biosynthesis I
BioCyc:HUMAN_PWY-7851 coenzyme A biosynthesis II (eukaryotic)
BioCyc:BSUB_COA-PWY coenzyme A biosynthesis I (prokaryotic)
BioCyc:ECOL316407_COA-PWY coenzyme A biosynthesis
BioCyc:HUMAN_COA-PWY-1 coenzyme A biosynthesis
BioCyc:META_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:META_COA-PWY-1 superpathway of coenzyme A biosynthesis III (mammals)
BioCyc:MTBCDC1551_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:MOUSE_COA-PWY coenzyme A biosynthesis
BioCyc:CORYNE_COA-PWY coenzyme A biosynthesis I
BioCyc:META_COA-PWY coenzyme A biosynthesis I (prokaryotic)
BioCyc:ECO_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:YEAST_COA-PWY coenzyme A biosynthesis
BioCyc:MTBCDC1551_COA-PWY coenzyme A biosynthesis
BioCyc:ECOL199310_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:ECOL199310_COA-PWY coenzyme A biosynthesis
BioCyc:AGRO_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:BSUB_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:ECOL316407_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:CORYNE_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
PathBank:SMP0000848 Pantothenate and CoA Biosynthesis
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-199220 Vitamin B5 (pantothenate) metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-199220 Vitamin B5 (pantothenate) metabolism
Reactome:R-SCE-196783 Coenzyme A biosynthesis
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SCE-199220 Vitamin B5 (pantothenate) metabolism
Reactome:R-HSA-196783 Coenzyme A biosynthesis
Reactome:R-MMU-196783 Coenzyme A biosynthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
PathBank:SMP0002307 beta-Alanine Metabolism
PathBank:SMP0000027 Pantothenate and CoA Biosynthesis
PathBank:SMP0002364 Pantothenate and CoA Biosynthesis