EC: 2.6.1.85

aminodeoxychorismate synthase (chorismate:L-glutamine aminotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q8LPN3 [aminodeoxychorismate synthase(Enzyme) wildtype His-tagged]

Chorismate + L_Glutamine -> L_Glutamate + 4_Amino_4_deoxychorismate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris/HCl, 5 mM MgCl2
PH: 8
Temperature: 37
uniprot:Q8LPN3 [aminodeoxychorismate synthase(Enzyme) wildtype His-tagged]

L_Glutamine + Chorismate -> L_Glutamate + 4_Amino_4_deoxychorismate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris/HCl, 5 mM MgCl2
PH: 8
Temperature: 37
uniprot:Q8LPN3 [aminodeoxychorismate synthase(Enzyme) wildtype His-tagged]

NH3 + Chorismate -> H2O + 4_Amino_4_deoxychorismate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM triethanolamine, 5 mM MgCl2, 0.07 µg/µl PabC extract
PH: 8
Temperature: 37
uniprot:P05041 [aminodeoxychorismate synthase(Enzyme) wildtype]

NH3 + Chorismate -> H2O + 4_Amino_4_deoxychorismate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 10 % glycerol, 2 mM DTT, 16 µM ADCL
PH: 8.5
Temperature: 37
uniprot:P28820 [aminodeoxychorismate synthase(Enzyme) wildtype]

L_Glutamine + Chorismate -> 2_Amino_2_deoxyisochorismic_acid + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 10 % glycerol, 2 mM DTT, 16 µM ADCL
PH: 8.5
Temperature: 37
uniprot:P28820 [aminodeoxychorismate synthase(Enzyme) wildtype]

Chorismate + NH3 -> 2_Amino_2_deoxyisochorismic_acid + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 10 % glycerol, 2 mM DTT, 16 µM ADCL
PH: 8.5
Temperature: 37
uniprot:Q8LPN3 [aminodeoxychorismate synthase(Enzyme) wildtype His-tagged]

L_Glutamine + Chorismate -> 4_Amino_4_deoxychorismate + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris/HCl, 5 mM MgCl2
PH: 8
Temperature: 37
- [aminodeoxychorismate synthase(Enzyme) wildtype]

Chorismate + L_Glutamine -> 4_Amino_4_deoxychorismate + L_Glutamate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 5 % glycerol, 5 mM DTT, 5 mM MgCl2, 1000 U/ml ADC lyase
PH: 7.5
Temperature: 37
- [aminodeoxychorismate synthase(Enzyme) wildtype]

Chorismate + L_Glutamine -> 4_Amino_4_deoxychorismate + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 % glycerol, 5 mM DTT, 5 mM MgCl2, 1000 U/ml ADC lyase
PH: 7.5
Temperature: 37
- [aminodeoxychorismate synthase(Enzyme) wildtype]

NH3 + Chorismate -> H2O + 4_Amino_4_deoxychorismate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM triethanolamine, 5 % glycerol, 5 mM DTT, 5 mM MgCl2, 1000 U/ml ADC lyase
PH: 8.5
Temperature: 37
- [aminodeoxychorismate synthase(Enzyme) wildtype]

NH3 + Chorismate -> 4_Amino_4_deoxychorismate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM triethanolamine, 5 % glycerol, 5 mM DTT, 5 mM MgCl2, 1000 U/ml ADC lyase
PH: 8.5
Temperature: 37
uniprot:P05041 [aminodeoxychorismate synthase(Enzyme) wildtype]

Chorismate + NH3 -> H2O + 4_Amino_4_deoxychorismate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM triethanolamine, 5 % glycerol, 5 mM DTT, 5 mM MgCl2, 1000 U/ml ADC lyase
PH: 8.5
Temperature: 37
uniprot:P05041 [aminodeoxychorismate synthase(Enzyme) wildtype]

Chorismate + NH3 -> 4_Amino_4_deoxychorismate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM triethanolamine, 5 % glycerol, 5 mM DTT, 5 mM MgCl2, 1000 U/ml ADC lyase
PH: 8.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ECO_PWY-6543 4-aminobenzoate biosynthesis
BioCyc:YEAST_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ARA_PWY-6543 4-aminobenzoate biosynthesis
BioCyc:ARA_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:BSUB_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ECOL316407_PWY-6543 4-aminobenzoate biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
BioCyc:BSUB_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ECOL316407_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:META_PWY-7575 superpathway of candicidin biosynthesis
BioCyc:YEAST_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:YEAST_PWY-6543 4-aminobenzoate biosynthesis
BioCyc:META_PWY-6543 4-aminobenzoate biosynthesis
BioCyc:META_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:YEAST_PWY3O-1874 p-aminobenzoate biosynthesis
BioCyc:BSUB_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:BSUB_PWY-6543 4-aminobenzoate biosynthesis
BioCyc:BSUB_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
PathBank:SMP0000925 Folate Biosynthesis
PlantCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:TOMATO_PWY-6612 superpathway of tetrahydrofolate biosynthesis
PlantCyc:ARA_PWY-6612 superpathway of tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:TOMATO_PWY-6543 4-aminobenzoate biosynthesis I
PlantCyc:ARA_PWY-6543 4-aminobenzoate biosynthesis
PlantCyc:TOMATO_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:PLANT_PWY-6543 4-aminobenzoate biosynthesis
PlantCyc:PLANT_PWY-6612 superpathway of tetrahydrofolate biosynthesis
PathBank:SMP0012014 Folate Biosynthesis
PathBank:SMP0002334 Tetrahydrofolate Biosynthesis