EC: 2.5.1.3

thiamine phosphate synthase (4-amino-2-methyl-5-diphosphomethylpyrimidine:2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate 4-amino-2-methylpyrimidine-5-methenyltransferase (decarboxylating))

enzyme lambda metabolic reaction experiment
uniprot:Q2UVH9 [ATP]

2_Methyl_4_amino_5_hydroxymethylpyrimidine_diphosphate + 4_Methyl_5_(2_phosphoethyl)_thiazole -> Thiamine_monophosphate + Diphosphate		 (( (Vmax * S) ) / (( (Km + S) ) + (( (S * I) ) / Ki))) buffer: 50 mM Tris/HCl, 10 mM MgCl2
PH: 8
Temperature: 37
uniprot:Q2UVH9 [thiamine-phosphate diphosphorylase(Enzyme) wildtype]

4_Methyl_5_(2_phosphoethyl)_thiazole + 2_Methyl_4_amino_5_hydroxymethylpyrimidine_diphosphate -> Diphosphate + Thiamine_monophosphate		 (( (Vmax * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 50 mM Tris/HCl, 1 mM ATP, 10 mM MgCl2
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6897 thiamine salvage II
BioCyc:META_PWY-6893 thiamine diphosphate biosynthesis II (Bacillus)
BioCyc:ECO_PWY-6897 thiamine salvage II
BioCyc:YEAST_PWY-7353 4-methyl-5(β-hydroxyethyl)thiazole salvage
BioCyc:YEAST_PWY-7356 thiamin salvage IV
BioCyc:ARA_PWY-7356 thiamine salvage IV (yeast)
BioCyc:ARA_PWY-6910 hydroxymethylpyrimidine salvage
BioCyc:ARA_PWY-6890 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis
BioCyc:ARA_PWY-6908 thiamine diphosphate biosynthesis IV (eukaryotes)
BioCyc:MTBCDC1551_PWY-6897 thiamine salvage II
BioCyc:MTBCDC1551_PWY-6907 thiamine diphosphate biosynthesis III (Staphylococcus)
BioCyc:MTBCDC1551_PWY-6893 thiamine diphosphate biosynthesis II (Bacillus)
BioCyc:ANTHRA_PWY-6894 thiamine diphosphate biosynthesis I (E. coli)
BioCyc:PDIF272563_PWY-6907 thiamine diphosphate biosynthesis III (Staphylococcus)
BioCyc:BTHE_PWY-6907 thiamin diphosphate biosynthesis III (Staphylococcus)
BioCyc:META_PWY-7356 thiamine salvage IV (yeast)
BioCyc:META_PWY-6890 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis
BioCyc:META_PWY-6908 thiamine diphosphate biosynthesis IV (eukaryotes)
BioCyc:YEAST_PWY-6908 thiamin diphosphate biosynthesis IV (eukaryotes)
BioCyc:MTBH37RV_PWY-6893 thiamine diphosphate biosynthesis II (Bacillus)
BioCyc:MTBCDC1551_PWY-6894 thiamine diphosphate biosynthesis I (E. coli)
BioCyc:HPY_PWY-7353 4-methyl-5(β-hydroxyethyl)thiazole salvage
BioCyc:ANTHRA_PWY-6897 thiamine salvage II
BioCyc:AGRO_PWY-6897 thiamine salvage II
BioCyc:BSUB_PWY-6897 thiamine salvage II
BioCyc:BSUB_PWY-6893 thiamine diphosphate biosynthesis II (Bacillus)
BioCyc:ECOL316407_PWY-6894 thiamin diphosphate biosynthesis I (E. coli)
BioCyc:BTHE_PWY-6897 thiamin salvage II
BioCyc:BTHE_PWY-6893 thiamin diphosphate biosynthesis II (Bacillus)
BioCyc:BTHE_PWY-7353 4-methyl-5(β-hydroxyethyl)thiazole salvage (yeast)
BioCyc:META_PWY-6910 hydroxymethylpyrimidine salvage
BioCyc:META_THISYNARA-PWY superpathway of thiamine diphosphate biosynthesis III (eukaryotes)
BioCyc:META_PWY-7357 thiamine formation from pyrithiamine and oxythiamine (yeast)
BioCyc:ECOO157_PWY-6897 thiamin salvage II
BioCyc:AGRO_PWY-6894 thiamine diphosphate biosynthesis I (E. coli)
BioCyc:PDIF272563_PWY-6897 thiamine salvage II
BioCyc:BTHE_PWY-6894 thiamin diphosphate biosynthesis I (E. coli)
BioCyc:CORYNE_PWY-6893 thiamine diphosphate biosynthesis II (Bacillus)
BioCyc:META_PWY-6894 thiamine diphosphate biosynthesis I (E. coli)
BioCyc:ECO_PWY-6894 thiamine diphosphate biosynthesis I (E. coli)
BioCyc:YEAST_THISYNARA-PWY-1 superpathway of thiamin diphosphate biosynthesis III (eukaryotes)
BioCyc:YEAST_PWY-7357 thiamin formation from pyrithiamine and oxythiamine
BioCyc:MTBH37RV_PWY-6894 thiamine diphosphate biosynthesis I (E. coli)
BioCyc:PDIF272563_PWY-6893 thiamine diphosphate biosynthesis II (Bacillus)
BioCyc:PDIF272563_PWY-6894 thiamine diphosphate biosynthesis I (E. coli)
BioCyc:CORYNE_PWY-6894 thiamine diphosphate biosynthesis I (E. coli)
BioCyc:GCF_000013425_PWY-6907 thiamine diphosphate biosynthesis III (Staphylococcus)
BioCyc:GCF_000013425_PWY-6897 thiamine salvage II
PathBank:SMP0002042 Thiamin Diphosphate Biosynthesis
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2867929 Cofactor biosyntheses
Plant Reactome:R-OSA-1119629 Thiamin biosynthesis
PlantCyc:ARA_THISYNARA-PWY superpathway of thiamine diphosphate biosynthesis III (eukaryotes)
PlantCyc:PLANT_PWY-6910 hydroxymethylpyrimidine salvage
PlantCyc:PLANT_PWY-6890 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis
PlantCyc:PLANT_THISYNARA-PWY superpathway of thiamine diphosphate biosynthesis III (eukaryotes)
PlantCyc:PLANT_PWY-6908 thiamine diphosphate biosynthesis IV (eukaryotes)
PlantCyc:ARA_PWY-6890 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis
PlantCyc:ARA_PWY-6908 thiamine diphosphate biosynthesis IV (eukaryotes)
PlantCyc:ARA_PWY-7356 thiamine salvage IV (yeast)
PlantCyc:ARA_PWY-6910 hydroxymethylpyrimidine salvage
PathBank:SMP0002385 Vitamin B1/Thiamine Metabolism
PathBank:SMP0122222 Thiamin Diphosphate Biosynthesis