thiamine diphosphate biosynthesis IV (eukaryotes)

Ajjawi I, Rodriguez Milla MA, Cushman J, Shintani DK. Thiamin pyrophosphokinase is required for thiamin cofactor activation in Arabidopsis. Plant Mol Biol. 2007 Sep;65(1-2):151–62. doi: 10.1007/s11103-007-9205-4. PMID: 17611796.; Nosaka K. High affinity of acid phosphatase encoded by PHO3 gene in Saccharomyces cerevisiae for thiamin phosphates. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1990 Feb;1037(2):147–54. doi: 10.1016/0167-4838(90)90160-h.

Metabolites

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Thiamine

Formula: C12H17N4OS (265.1123012)

CAS ID: 70-16-6

adenosine 5'-monophosphate(2-)

Formula: C10H12N5O7P (345.0474332)

CAS ID:

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

thiamine(1+) diphosphate(3-)

Formula: C12H16N4O7P2S (422.0214926)

CAS ID:

thiamine(1+) monophosphate(2-)

Formula: C12H16N4O4PS (343.06298460000005)

CAS ID: 532-40-1



Enzyme

EC Number name full name note
3.6.1.-
2.5.1.3 thiamine phosphate synthase 4-amino-2-methyl-5-diphosphomethylpyrimidine:2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate 4-amino-2-methylpyrimidine-5-methenyltransferase (decarboxylating)
2.7.1.49 hydroxymethylpyrimidine kinase ATP:4-amino-5-hydroxymethyl-2-methylpyrimidine 5-phosphotransferase
3.1.3.100 thiamine phosphate phosphatase thiamine phosphate phosphohydrolase


Proteins

Protein ID name full name