EC: 2.1.1.37

DNA (cytosine-5-)-methyltransferase (S-adenosyl-L-methionine:DNA (cytosine-5-)-methyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) mutant Q237H]

Cytosine_(in_DNA) + S_Adenosyl_L_methionine -> 5_Methylcytosine_(in_DNA) + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) mutant Q237L]

Cytosine_(in_DNA) + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + 5_Methylcytosine_(in_DNA)		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) mutant Q237A]

S_Adenosyl_L_methionine + Cytosine_(in_DNA) -> 5_Methylcytosine_(in_DNA) + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) wildtype]

Cytosine_(in_DNA) + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + 5_Methylcytosine_(in_DNA)		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) mutant Q237S]

Cytosine_(in_DNA) + S_Adenosyl_L_methionine -> 5_Methylcytosine_(in_DNA) + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) mutant Q237E]

Cytosine_(in_DNA) + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + 5_Methylcytosine_(in_DNA)		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) mutant Q237A]

Cytosine_(in_DNA) + S_Adenosyl_L_methionine -> 5_Methylcytosine_(in_DNA) + S_Adenosyl_L_homocysteine		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + Cytosine_(in_DNA) -> 5_Methylcytosine_(in_DNA) + S_Adenosyl_L_homocysteine		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) mutant Q237H]

S_Adenosyl_L_methionine + Cytosine_(in_DNA) -> S_Adenosyl_L_homocysteine + 5_Methylcytosine_(in_DNA)		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) mutant Q237L]

S_Adenosyl_L_methionine + Cytosine_(in_DNA) -> 5_Methylcytosine_(in_DNA) + S_Adenosyl_L_homocysteine		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) mutant Q237S]

Cytosine_(in_DNA) + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + 5_Methylcytosine_(in_DNA)		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P05102 [DNA (cytosine-5-)-methyltransferase(Enzyme) mutant Q237E]

Cytosine_(in_DNA) + S_Adenosyl_L_methionine -> 5_Methylcytosine_(in_DNA) + S_Adenosyl_L_homocysteine		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 10 mM Na2EDTA, 10 mM beta-mercaptoethanol, 200 µg/ml BSA
PH: 7.4
Temperature: 37

Pathways

pathway id name
Reactome:R-HSA-9700206 Signaling by ALK in cancer
Reactome:R-HSA-9725370 Signaling by ALK fusions and activated point mutants
Reactome:R-HSA-201556 Signaling by ALK
Reactome:R-HSA-9725371 Nuclear events stimulated by ALK signaling in cancer
Reactome:R-HSA-9701898 STAT3 nuclear events downstream of ALK signaling
Reactome:R-HSA-9845323 Regulation of endogenous retroelements by Piwi-interacting RNAs (piRNAs)
Reactome:R-HSA-9842860 Regulation of endogenous retroelements
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-2990846 SUMOylation
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-HSA-5334118 DNA methylation
Reactome:R-MMU-3214858 RMTs methylate histone arginines
Reactome:R-MMU-4655427 SUMOylation of DNA methylation proteins
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-2990846 SUMOylation
Reactome:R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
Reactome:R-HSA-4655427 SUMOylation of DNA methylation proteins
Reactome:R-HSA-3214858 RMTs methylate histone arginines
Reactome:R-HSA-212165 Epigenetic regulation of gene expression
Reactome:R-HSA-427413 NoRC negatively regulates rRNA expression
Reactome:R-MMU-3247509 Chromatin modifying enzymes
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-4655427 SUMOylation of DNA methylation proteins
Reactome:R-HSA-3108232 SUMO E3 ligases SUMOylate target proteins
Reactome:R-HSA-5663202 Diseases of signal transduction by growth factor receptors and second messengers
Reactome:R-HSA-4839726 Chromatin organization
Reactome:R-HSA-5250941 Negative epigenetic regulation of rRNA expression
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-MMU-2990846 SUMOylation
Reactome:R-RNO-4839726 Chromatin organization
Reactome:R-RNO-3108232 SUMO E3 ligases SUMOylate target proteins
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-3247509 Chromatin modifying enzymes
Reactome:R-MMU-4839726 Chromatin organization
Reactome:R-MMU-3108232 SUMO E3 ligases SUMOylate target proteins
Reactome:R-RNO-3247509 Chromatin modifying enzymes
Reactome:R-RNO-3214858 RMTs methylate histone arginines
PathBank:SMP0000177 Cystathionine beta-Synthase Deficiency
PathBank:SMP0000340 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0120471 Cystathionine beta-Synthase Deficiency
PathBank:SMP0120487 Glycine N-Methyltransferase Deficiency
PathBank:SMP0120519 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120527 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0000033 Methionine Metabolism
PathBank:SMP0000222 Glycine N-Methyltransferase Deficiency
PathBank:SMP0063637 Methionine Metabolism
PathBank:SMP0120634 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0000341 Hypermethioninemia
PathBank:SMP0000221 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120498 Hypermethioninemia
PathBank:SMP0000214 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0000570 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type