EC: 6.3.4.15

biotin-[biotin carboxyl-carrier protein] ligase (biotin:apo-[carboxyl-carrier protein] ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P50747 [biotin---[acetyl-CoA-carboxylase] ligase(Enzyme) wildtype]

Apo_[acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + ATP + Biotin -> Diphosphate + AMP + [Acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)]		 (( (Vmax * A) ) / (Km + A)) buffer: 125 mM Tris-HCl, 8 mM MgCl2, 50 mM KCl, 1.4 mM EDTA, 2.5 mM mercaptoethanol
PH: 8
Temperature: 30
uniprot:P50747 [biotin---[acetyl-CoA-carboxylase] ligase(Enzyme) mutant L216R]

Apo_[acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + ATP + Biotin -> Diphosphate + [Acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 125 mM Tris-HCl, 8 mM MgCl2, 50 mM KCl, 1.4 mM EDTA, 2.5 mM mercaptoethanol
PH: 8
Temperature: 30
uniprot:P50747 [biotin---[acetyl-CoA-carboxylase] ligase(Enzyme) mutant V333E]

Apo_[acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + ATP + Biotin -> AMP + Diphosphate + [Acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)]		 (( (Vmax * A) ) / (Km + A)) buffer: 125 mM Tris-HCl, 8 mM MgCl2, 50 mM KCl, 1.4 mM EDTA, 2.5 mM mercaptoethanol
PH: 8
Temperature: 30
uniprot:P50747 [biotin---[acetyl-CoA-carboxylase] ligase(Enzyme) mutant delThr610]

Apo_[acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + Biotin + ATP -> [Acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + Diphosphate + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 125 mM Tris-HCl, 8 mM MgCl2, 50 mM KCl, 1.4 mM EDTA, 2.5 mM mercaptoethanol
PH: 8
Temperature: 30
uniprot:P50747 [biotin---[acetyl-CoA-carboxylase] ligase(Enzyme) mutant L237P]

Apo_[acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + Biotin + ATP -> Diphosphate + AMP + [Acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)]		 (( (Vmax * A) ) / (Km + A)) buffer: 125 mM Tris-HCl, 8 mM MgCl2, 50 mM KCl, 1.4 mM EDTA, 2.5 mM mercaptoethanol
PH: 8
Temperature: 30
uniprot:P50747 [biotin---[acetyl-CoA-carboxylase] ligase(Enzyme) mutant R183P]

Apo_[acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + ATP + Biotin -> [Acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + Diphosphate + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 125 mM Tris-HCl, 8 mM MgCl2, 50 mM KCl, 1.4 mM EDTA, 2.5 mM mercaptoethanol
PH: 8
Temperature: 30
uniprot:P50747 [biotin---[acetyl-CoA-carboxylase] ligase(Enzyme) mutant G581S]

Apo_[acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + Biotin + ATP -> AMP + [Acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 125 mM Tris-HCl, 8 mM MgCl2, 50 mM KCl, 1.4 mM EDTA, 2.5 mM mercaptoethanol
PH: 8
Temperature: 30
uniprot:P50747 [biotin---[acetyl-CoA-carboxylase] ligase(Enzyme) mutant V363D]

Apo_[acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + ATP + Biotin -> [Acetyl_CoA:carbon_dioxide_ligase_(ADP_forming)] + Diphosphate + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 125 mM Tris-HCl, 8 mM MgCl2, 50 mM KCl, 1.4 mM EDTA, 2.5 mM mercaptoethanol
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY0-1264 biotin-carboxyl carrier protein assembly
BioCyc:META_PWY0-1264 biotin-carboxyl carrier protein assembly
BioCyc:ECO_PWY0-1264 biotin-carboxyl carrier protein assembly
BioCyc:YEAST_PWY0-1264 biotin-carboxyl carrier protein assembly
BioCyc:MTBH37RV_PWY0-1264 biotin-carboxyl carrier protein assembly
BioCyc:BSUB_PWY0-1264 biotin-carboxyl carrier protein assembly
PathBank:SMP0000785 Biotin Metabolism
PathBank:SMP0002079 Biotin-Carboxyl Carrier Protein Assembly
PlantCyc:ARA_PWY0-1264 biotin-carboxyl carrier protein assembly
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196780 Biotin transport and metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-196780 Biotin transport and metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SCE-196780 Biotin transport and metabolism
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-196780 Biotin transport and metabolism
WikiPathways:WP5031 Biotin metabolism, including IMDs
PathBank:SMP0120460 Biotinidase Deficiency
PathBank:SMP0000174 Biotinidase Deficiency
PathBank:SMP0000564 Multiple Carboxylase Deficiency, Neonatal or Early Onset Form
PathBank:SMP0120628 Multiple Carboxylase Deficiency, Neonatal or Early Onset Form
PathBank:SMP0063602 Biotin Metabolism
PathBank:SMP0000066 Biotin Metabolism