EC: 5.1.1.7

diaminopimelate epimerase (LL-2,6-diaminoheptanedioate 2-epimerase)

enzyme lambda metabolic reaction experiment
uniprot:P9WP19 [diaminopimelate epimerase(Enzyme) mutant C87S His-tagged]

meso_2,6_Diaminoheptanedioate -> LL_2,6_Diaminoheptanedioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 30
uniprot:P9WP19 [diaminopimelate epimerase(Enzyme) mutant C226S His-tagged]

meso_2,6_Diaminoheptanedioate -> LL_2,6_Diaminoheptanedioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 30
uniprot:P0A6K1 [diaminopimelate epimerase(Enzyme) wildtype His6-tagged]

LL_2,6_Diaminoheptanedioate -> meso_2,6_Diaminoheptanedioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.3 mM NADP+, 1 mM DTT, 1.8 µM DAPDH from C. glutanicum
PH: 7.8
Temperature: 30
uniprot:P0A6K1 [diaminopimelate epimerase(Enzyme) wildtype His6-tagged]

LL_2,6_Diaminoheptanedioate -> meso_2,6_Diaminoheptanedioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.3 mM NADP+, 1 mM DTT, 1.8 µM DAPDH from C. glutanicum
PH: 7.8
Temperature: 30
uniprot:P9WP19 [diaminopimelate epimerase(Enzyme) wildtype His-tagged]

meso_2,6_Diaminoheptanedioate -> LL_2,6_Diaminoheptanedioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 1 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 30
uniprot:Q9X1L0 [diaminopimelate epimerase(Enzyme) wildtype C-terminal His-tagged]

LL_2,6_Diaminoheptanedioate -> meso_2,6_Diaminoheptanedioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM DTT
PH: 7.5
Temperature: 90
uniprot:Q9X1L0 [diaminopimelate epimerase(Enzyme) wildtype C-terminal His-tagged]

meso_2,6_Diaminoheptanedioate -> LL_2,6_Diaminoheptanedioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM DTT
PH: 7.5
Temperature: 90

Pathways

pathway id name
BioCyc:META_PWY0-781 aspartate superpathway
BioCyc:HPY_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ECOL316407_P4-PWY superpathway of lysine, threonine and methionine biosynthesis I
BioCyc:ECOL316407_PWY0-781 aspartate superpathway
BioCyc:CAULONA1000_PWY0-781 aspartate superpathway
BioCyc:CAULONA1000_DAPLYSINESYN-PWY lysine biosynthesis I
BioCyc:CORYNE_PWY0-781 aspartate superpathway
BioCyc:META_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ECO_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:ECO_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ARA_PWY-5097 L-lysine biosynthesis VI
BioCyc:CAULO_DAPLYSINESYN-PWY lysine biosynthesis I
BioCyc:ANTHRA_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:ECOL316407_DAPLYSINESYN-PWY lysine biosynthesis I
BioCyc:CAULONA1000_P4-PWY superpathway of lysine, threonine and methionine biosynthesis I
BioCyc:CORYNE_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ARA_PWY-724 superpathway of L-lysine, L-threonine and L-methionine biosynthesis II
BioCyc:MTBCDC1551_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ANTHRA_PWY0-781 aspartate superpathway
BioCyc:BSUB_PWY-2941 L-lysine biosynthesis II
BioCyc:META_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:ECO_PWY0-781 aspartate superpathway
BioCyc:MTBH37RV_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ANTHRA_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:SYNEL_PWY-5097 L-lysine biosynthesis VI
BioCyc:CORYNE_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
PathBank:SMP0000794 Lysine Biosynthesis
Plant Reactome:R-OSA-1119419 Lysine biosynthesis VI
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744343 Amino acid metabolism
Plant Reactome:R-OSA-1119273 Lysine biosynthesis I
Plant Reactome:R-OSA-5655122 Amino acid biosynthesis
Plant Reactome:R-OSA-1119283 Lysine biosynthesis II
PlantCyc:PLANT_PWY-724 superpathway of L-lysine, L-threonine and L-methionine biosynthesis II
PlantCyc:ARA_PWY-5097 L-lysine biosynthesis VI
PlantCyc:ARA_PWY-724 superpathway of L-lysine, L-threonine and L-methionine biosynthesis II
PlantCyc:PLANT_PWY-5097 L-lysine biosynthesis VI
PathBank:SMP0002434 Lysine Metabolism
PathBank:SMP0121266 Lysine Biosynthesis