EC: 4.2.99.20

2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate pyruvate-lyase [(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate-forming])

enzyme lambda metabolic reaction experiment
uniprot:P37355 [2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase(Enzyme) wildtype His6-tagged]

5_Enolpyruvoyl_6_hydroxy_2_succinyl_cyclohex_3_ene_1_carboxylate -> Pyruvate + 2_Succinyl_6_hydroxy_2,4_cyclohexadiene_1_carboxylate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.1% BSA
PH: 7
Temperature: 37
uniprot:P37355 [2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase(Enzyme) wildtype His6-tagged]

5_Enolpyruvoyl_6_hydroxy_2_succinyl_cyclohex_3_ene_1_carboxylate -> 2_Succinyl_6_hydroxy_2,4_cyclohexadiene_1_carboxylate + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.1% BSA
PH: 7
Temperature: 25
uniprot:P37355 [2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase(Enzyme) mutant D210A His6-tagged]

5_Enolpyruvoyl_6_hydroxy_2_succinyl_cyclohex_3_ene_1_carboxylate -> 2_Succinyl_6_hydroxy_2,4_cyclohexadiene_1_carboxylate + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.1% BSA
PH: 7
Temperature: 25
uniprot:P37355 [2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase(Enzyme) mutant H232A His6-tagged]

5_Enolpyruvoyl_6_hydroxy_2_succinyl_cyclohex_3_ene_1_carboxylate -> 2_Succinyl_6_hydroxy_2,4_cyclohexadiene_1_carboxylate + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.1% BSA
PH: 7
Temperature: 25
uniprot:P37355 [2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase(Enzyme) mutant S86A His6-tagged]

5_Enolpyruvoyl_6_hydroxy_2_succinyl_cyclohex_3_ene_1_carboxylate -> 2_Succinyl_6_hydroxy_2,4_cyclohexadiene_1_carboxylate + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.1% BSA
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
BioCyc:ECOL199310_PWY-5838 superpathway of menaquinol-8 biosynthesis I
BioCyc:ECOL316407_PWY-5837 1,4-dihydroxy-2-naphthoate biosynthesis I
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-5861 superpathway of demethylmenaquinol-8 biosynthesis I
BioCyc:META_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
BioCyc:META_PWY-5838 superpathway of menaquinol-8 biosynthesis I
BioCyc:ARA_PWY-5791 1,4-dihydroxy-2-naphthoate biosynthesis II (plants)
BioCyc:ECOL199310_PWY-5837 1,4-dihydroxy-2-naphthoate biosynthesis I
BioCyc:ECOL199310_PWY-5861 superpathway of demethylmenaquinol-8 biosynthesis
BioCyc:BSUB_PWY-5840 superpathway of menaquinol-7 biosynthesis
BioCyc:ECO_PWY-5838 superpathway of menaquinol-8 biosynthesis I
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ARA_PWY-5837 1,4-dihydroxy-2-naphthoate biosynthesis
BioCyc:ECOL199310_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:BSUB_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
BioCyc:ECOL316407_PWY-5838 superpathway of menaquinol-8 biosynthesis I
PathBank:SMP0001911 Menaquinol Biosythesis
PlantCyc:ARA_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
PlantCyc:ARA_PWY-5791 1,4-dihydroxy-2-naphthoate biosynthesis II (plants)
PlantCyc:PLANT_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
PlantCyc:ARA_PWY-5863 superpathway of phylloquinol biosynthesis
PlantCyc:PLANT_PWY-5791 1,4-dihydroxy-2-naphthoate biosynthesis II (plants)
PlantCyc:PLANT_PWY-5863 superpathway of phylloquinol biosynthesis