EC: 4.2.1.51

prephenate dehydratase (prephenate hydro-lyase (decarboxylating; phenylpyruvate-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q44104 [Tyrosine]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [Tyrosine]

Prephenate -> CO2 + Phenylpyruvate + H2O		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [Tyrosine]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [Phenylalanine]

Prephenate -> Phenylpyruvate + CO2 + H2O		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [Phenylalanine]

Prephenate -> H2O + CO2 + Phenylpyruvate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [Phenylalanine]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [Phenylalanine]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [prephenate dehydratase(Enzyme) mutant]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [prephenate dehydratase(Enzyme) mutant]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [Tyrosine]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [Tyrosine]

Prephenate -> Phenylpyruvate + CO2 + H2O		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [Phenylalanine]

Prephenate -> CO2 + H2O + Phenylpyruvate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [Phenylalanine]

Prephenate -> H2O + CO2 + Phenylpyruvate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q44104 [prephenate dehydratase(Enzyme) wildtype]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q58054 [prephenate dehydratase(Enzyme) wildtype]

Prephenate -> H2O + CO2 + Phenylpyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.5 M Borate, 17 µg/ml Phenylpyruvate tautomerase from bovine kidney
PH: 8
Temperature: 30
uniprot:P0A9J8 [L-Phenylalanine]

Prephenate -> H2O + CO2 + Phenylpyruvate		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / alphaKi))))) buffer: 20 mM Tris-Cl, 1.0 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [L-Phenylalanine]

Prephenate -> CO2 + Phenylpyruvate + H2O		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / alphaKi))))) buffer: 20 mM Tris-Cl, 1.0 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [L-Phenylalanine]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / alphaKi))))) buffer: 20 mM Tris-Cl, 1.0 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [L-Phenylalanine]

Prephenate -> CO2 + H2O + Phenylpyruvate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Cl, 1.0 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [L-Phenylalanine]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Cl, 1.0 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant PDT32]

Prephenate -> CO2 + Phenylpyruvate + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1.0 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) wildtype P-protein]

Prephenate -> Phenylpyruvate + CO2 + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1.0 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant PDT22]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1.0 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant P*300]

Prephenate -> CO2 + H2O + Phenylpyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1.0 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant P*285]

Prephenate -> CO2 + H2O + Phenylpyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1.0 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P10341 [prephenate dehydratase(Enzyme) wildtype pheA, His-tagged]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P10341 [prephenate dehydratase(Enzyme) mutant S99A pheA, His-tagged]

Prephenate -> CO2 + Phenylpyruvate + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P10341 [prephenate dehydratase(Enzyme) mutant S99M pheA, His-tagged]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P10341 [prephenate dehydratase(Enzyme) mutant S99C pheA, His-tagged]

Prephenate -> Phenylpyruvate + CO2 + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P10341 [prephenate dehydratase(Enzyme) mutant S99T pheA, His-tagged]

Prephenate -> Phenylpyruvate + CO2 + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P96240 [prephenate dehydratase(Enzyme) wildtype Rv3838c]

Prephenate -> H2O + CO2 + Phenylpyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 0.01% BSA, 1 mM DTT
PH: 8.2
Temperature: 37
uniprot:P10341 [prephenate dehydratase(Enzyme) mutant F185Y pheA, His-tagged]

Prephenate -> H2O + CO2 + Phenylpyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P10341 [prephenate dehydratase(Enzyme) mutant T183S pheA, His-tagged]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P10341 [prephenate dehydratase(Enzyme) mutant R184L pheA, His-tagged]

Prephenate -> Phenylpyruvate + CO2 + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P10341 [prephenate dehydratase(Enzyme) mutant E64D pheA, His-tagged]

Prephenate -> Phenylpyruvate + CO2 + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) wildtype]

Prephenate -> CO2 + Phenylpyruvate + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant C216S]

Prephenate -> CO2 + H2O + Phenylpyruvate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant C216A]

Prephenate -> H2O + CO2 + Phenylpyruvate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant T278S]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant E232A]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant E159A]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant E159A/E232A]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant N160A]

Prephenate -> H2O + CO2 + Phenylpyruvate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant S208C]

Prephenate -> CO2 + H2O + Phenylpyruvate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant W226A]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant S208A]

Prephenate -> Phenylpyruvate + H2O + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant Q215A]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) wildtype PDT20 (residues 101-285 of the P-protein)]

Prephenate -> CO2 + H2O + Phenylpyruvate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37
uniprot:P0A9J8 [prephenate dehydratase(Enzyme) mutant W226L]

Prephenate -> H2O + CO2 + Phenylpyruvate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-Cl, 1 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 8.2
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:META_PHESYN L-phenylalanine biosynthesis I
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_PHESYN L-phenylalanine biosynthesis
BioCyc:ARA_PWY-3481 superpathway of L-phenylalanine and L-tyrosine biosynthesis
BioCyc:ECOO157_PWY-6630 superpathway of tyrosine biosynthesis
BioCyc:BSUB_PHESYN L-phenylalanine biosynthesis I
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:CORYNE_PHESYN L-phenylalanine biosynthesis I
BioCyc:META_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:ARA_PWY-7432 L-phenylalanine biosynthesis III (cytosolic, plants)
BioCyc:ECOO157_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:META_TYRSYN L-tyrosine biosynthesis I
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:MTBH37RV_PHESYN L-phenylalanine biosynthesis I
BioCyc:ECOO157_PWY-6628 superpathway of phenylalanine biosynthesis
BioCyc:CORYNE_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:CORYNE_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:ECO_TYRSYN L-tyrosine biosynthesis I
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PHESYN L-phenylalanine biosynthesis I
BioCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:ARA_PWY-3462 L-phenylalanine biosynthesis II
BioCyc:ECOO157_TYRSYN tyrosine biosynthesis I
BioCyc:ECOO157_PHESYN phenylalanine biosynthesis I
BioCyc:ECOL316407_TYRSYN tyrosine biosynthesis I
BioCyc:ECOL316407_PHESYN phenylalanine biosynthesis I
PathBank:SMP0000827 Phenylalanine Biosynthesis
PathBank:SMP0000938 Phenylalanine Metabolism
PlantCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
PlantCyc:ARA_PWY-3481 superpathway of L-phenylalanine and L-tyrosine biosynthesis
PlantCyc:ARA_PWY-7432 L-phenylalanine biosynthesis III (cytosolic, plants)
PlantCyc:ARA_PWY-3462 L-phenylalanine biosynthesis II
PlantCyc:PLANT_PWY-3462 L-phenylalanine biosynthesis II
PlantCyc:PLANT_PWY-3481 superpathway of L-phenylalanine and L-tyrosine biosynthesis
PathBank:SMP0002442 Phenylalanine Metabolism
PathBank:SMP0002349 Phenylalanine Metabolism