EC: 1.1.1.159

7alpha-hydroxysteroid dehydrogenase (7alpha-hydroxysteroid:NAD+ 7-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q5LA59 [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

3alpha,7alpha,12alpha_Trihydroxy_5beta_cholanate + NAD+ -> 3alpha,12alpha_Dihydroxy_7_oxo_5beta_cholanate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM sodium pyrophosphate
PH: 8.8
Temperature: 22
uniprot:Q5LA59 [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

Chenodeoxycholate + NAD+ -> H+ + 3alpha_Hydroxy_7_oxo_5beta_cholanate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM sodium pyrophosphate
PH: 8.8
Temperature: 22
uniprot:Q5LA59 [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

NAD+ + 3alpha,7alpha,12alpha_Trihydroxy_5beta_cholanate -> H+ + 3alpha,12alpha_Dihydroxy_7_oxo_5beta_cholanate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M disodium phosphate
PH: 7
Temperature: 22
- [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

3alpha,7alpha,12alpha_Trihydroxy_5beta_cholanate + NADP+ -> NADPH + 3alpha,12alpha_Dihydroxy_7_oxo_5beta_cholanate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM sodium pyrophosphate
PH: 9.5
Temperature: 22
- [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

NADP+ + 3alpha,7alpha,12alpha_Trihydroxy_5beta_cholanate -> H+ + 3alpha,12alpha_Dihydroxy_7_oxo_5beta_cholanate + NADPH		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M disodium phosphate
PH: 7
Temperature: 22
- [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

NADP+ + 3alpha,7alpha,12alpha_Trihydroxy_5beta_cholanate -> NADPH + 3alpha,12alpha_Dihydroxy_7_oxo_5beta_cholanate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM sodium pyrophosphate
PH: 8.8
Temperature: 22
- [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

Chenodeoxycholate + NADP+ -> NADPH + H+ + 3alpha_Hydroxy_7_oxo_5beta_cholanate		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM sodium pyrophosphate
PH: 8.8
Temperature: 22
uniprot:Q5LA59 [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

3alpha,7alpha,12alpha_Trihydroxy_5beta_cholanate + NAD+ -> NADH + 3alpha,12alpha_Dihydroxy_7_oxo_5beta_cholanate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM sodium pyrophosphate
PH: 9.5
Temperature: 22
- [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

NAD+ + 3alpha,7alpha,12alpha_Trihydroxy_5beta_cholanate -> 3alpha,12alpha_Dihydroxy_7_oxo_5beta_cholanate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM sodium pyrophosphate
PH: 9.5
Temperature: 22
- [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

3alpha,7alpha,12alpha_Trihydroxy_5beta_cholanate + NAD+ -> H+ + 3alpha,12alpha_Dihydroxy_7_oxo_5beta_cholanate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM sodium pyrophosphate
PH: 9.5
Temperature: 22
- [7alpha-hydroxysteroid dehydrogenase(Enzyme) wildtype]

3alpha,7alpha,12alpha_Trihydroxy_5beta_cholanate + NADP+ -> H+ + 3alpha,12alpha_Dihydroxy_7_oxo_5beta_cholanate + NADPH		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM sodium pyrophosphate
PH: 9.5
Temperature: 22

Pathways

pathway id name
BioCyc:HUMAN_ILEUDEG-PWY isoleucine degradation
BioCyc:META_FAO-PWY fatty acid β-oxidation I
BioCyc:META_ILEUDEG-PWY L-isoleucine degradation I
BioCyc:MOUSE_PWY3DJ-0 isoleucine degradation
BioCyc:MOUSE_PWY-5109 2-methylbutyrate biosynthesis
BioCyc:HUMAN_FAO-PWY fatty acid β-oxidation
BioCyc:MOUSE_FAO-PWY fatty acid β-oxidation I
BioCyc:MOUSE_PWY-5136 fatty acid β-oxidation II (core pathway)
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-70895 Branched-chain amino acid catabolism
Reactome:R-HSA-72306 tRNA processing
Reactome:R-HSA-6785470 tRNA processing in the mitochondrion
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-70895 Branched-chain amino acid catabolism
Reactome:R-RNO-70895 Branched-chain amino acid catabolism
Reactome:R-BTA-70895 Branched-chain amino acid catabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-8953854 Metabolism of RNA
Reactome:R-HSA-6787450 tRNA modification in the mitochondrion
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-72312 rRNA processing
Reactome:R-HSA-8868766 rRNA processing in the mitochondrion
WikiPathways:WP4686 Leucine, isoleucine and valine metabolism
PathBank:SMP0120440 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120444 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120516 Maple Syrup Urine Disease
PathBank:SMP0120589 Isovaleric Acidemia
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0000544 Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase Deficiency (LCHAD)
PathBank:SMP0000568 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0063612 Fatty Acid Elongation In Mitochondria
PathBank:SMP0120439 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120443 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120459 beta-Ketothiolase Deficiency
PathBank:SMP0120503 Isovaleric Aciduria
PathBank:SMP0120523 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120588 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120608 Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase Deficiency (LCHAD)
PathBank:SMP0120632 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0000054 Fatty Acid Elongation in Mitochondria
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0063641 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0063689 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120441 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120525 Methylmalonic Aciduria
PathBank:SMP0120537 Propionic Acidemia
PathBank:SMP0120586 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0120442 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120587 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000480 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000635 Valproic Acid Metabolism Pathway