N-glycan antennae elongation in the medial/trans-Golgi

Ito H, Chiba Y, Kameyama A, Sato T, Narimatsu H. In vitro and in vivo enzymatic syntheses and mass spectrometric database for N-glycans and o-glycans. Methods Enzymol. 2010;478():127–49. doi: 10.1016/s0076-6879(10)78005-8. PMID: 20816477.; Hossler P, Goh LT, Lee MM, Hu WS. GlycoVis: visualizing glycan distribution in the protein N-glycosylation pathway in mammalian cells. Biotechnol Bioeng. 2006 Dec 05;95(5):946–60. doi: 10.1002/bit.21062. PMID: 16807922.; Schachter H. The joys of HexNAc. The synthesis and function of N- and O-glycan branches. Glycoconj J. 2000 Jul;17(7-9):465–83. doi: 10.1023/a:1011010206774. PMID: 11421343.; Kornfeld R, Kornfeld S. Assembly of asparagine-linked oligosaccharides. Annu Rev Biochem. 1985;54():631–64. doi: 10.1146/annurev.bi.54.070185.003215. PMID: 3896128.

Metabolites

D-Mannose

Formula: C6H12O6 (180.0633852)

CAS ID: 3458-28-4

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Manganese(2+)

Formula: Mn (54.938046)

CAS ID: 7439-96-5

Zinc cation

Formula: Zn (63.929145)

CAS ID: 23713-49-7

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0



Enzyme

EC Number name full name note
2.4.1.155 alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
2.4.1.244 N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase UDP-N-acetyl-D-galactosamine:N-acetyl-beta-D-glucosaminyl-group 4-beta-N-acetylgalactosaminyltransferase
2.4.1.68 glycoprotein 6-alpha-L-fucosyltransferase GDP-beta-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine) 6-alpha-L-fucosyltransferase
3.2.1.51 alpha-L-fucosidase alpha-L-fucoside fucohydrolase


Proteins

Protein ID name full name