- 数据之源，洞见之始

EC: 1.1.1.141

15-hydroxyprostaglandin dehydrogenase (NAD+) ((5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate:NAD+ 15-oxidoreductase)

enzyme	lambda	experiment
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q148H GST-fusion] Prostaglandin_E2 + NAD+ -> H+ + NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q148E GST-fusion] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH + H+	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q148N GST-fusion] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+ + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype GST-15-PGDH] NAD+ + Prostaglandin_E2 -> H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q148H GST-fusion] NAD+ + Prostaglandin_E2 -> H+ + NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype GST-15-PGDH] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH + H+	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q148E GST-fusion] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH + H+	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q148N GST-fusion] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH + H+	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype GST-fusion protein] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+ + NADH	`(( (( (kcat * E) ) * S) ) / (S + Km))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant T11S GST-fusion protein] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+ + NADH	`(( (( (kcat * E) ) * S) ) / (S + Km))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype GST-fusion protein] NAD+ + Prostaglandin_E2 -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+	`(( (( (kcat * E) ) * S) ) / (S + Km))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant T11S GST-fusion protein] Prostaglandin_E2 + NAD+ -> H+ + NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (kcat * E) ) * S) ) / (S + Km))`	buffer: 50 mM Tris-HCl, 0.1 mM DTT PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15R] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant W37K] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant W37R] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K] NAD+ + Prostaglandin_E2 -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15R] NAD+ + Prostaglandin_E2 -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] NAD+ + Prostaglandin_E2 -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant W37K] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15R-W37K] Prostaglandin_E2 + NAD+ -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K-W37R] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K-W37K] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15R-W37R] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K-W37R] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K-W37K] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15R-W37K] Prostaglandin_E2 + NAD+ -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant W37R] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15R-W37R] Prostaglandin_E2 + NAD+ -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant A14S-Q15K-D36S-W37R] Prostaglandin_E2 + NADP+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADPH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15R-W37R] NADP+ + Prostaglandin_E2 -> NADPH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K-D36S-W37R] Prostaglandin_E2 + NADP+ -> NADPH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K-D36A-W37R] NADP+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADPH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K-W37R] Prostaglandin_E2 + NADP+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADPH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K-D36A-W37R] NADP+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADPH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K-W37R] Prostaglandin_E2 + NADP+ -> NADPH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15K-D36S-W37R] Prostaglandin_E2 + NADP+ -> NADPH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant Q15R-W37R] NADP+ + Prostaglandin_E2 -> NADPH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant A14S-Q15K-D36S-W37R] Prostaglandin_E2 + NADP+ -> NADPH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant I17A] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant I17L] Prostaglandin_E2 + NAD+ -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant I17V] NAD+ + Prostaglandin_E2 -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] Prostaglandin_E2 + NAD+ -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant N91A] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant V186I] Prostaglandin_E2 + NAD+ -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant V186A] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant I17L] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant I17A] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] Prostaglandin_E2 + NAD+ -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant N91D] Prostaglandin_E2 + NAD+ -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant I17V] Prostaglandin_E2 + NAD+ -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant V186K] NAD+ + Prostaglandin_E2 -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant V186A] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant V186I] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant N91D] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant N91A] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
- [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant V186K] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 0.1 mM ditiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] NAD+ + Prostaglandin_E2 -> NADH + H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] Prostaglandin_E2 + NAD+ -> NADH + H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (Vmax * B) ) / (Km + B))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:O08699 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] NAD+ + Prostaglandin_E2 -> H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:O08699 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+ + NADH	`(( (Vmax * B) ) / (Km + B))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:Q8VCC1 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+ + NADH	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:O08699 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant chimeric 1-252_rat/253-266_human (P15428)] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH + H+	`(( (Vmax * B) ) / (Km + B))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] NAD+ + Prostaglandin_E2 -> H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant chimeric 1-252_human/253-266_rat (O08699)] NAD+ + Prostaglandin_E2 -> NADH + H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant chimeric 1-252_human/253-266_rat (O08699)] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH + H+	`(( (Vmax * B) ) / (Km + B))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant 255, C-terminal 11 residues deleted] NAD+ + Prostaglandin_E2 -> H+ + NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (Vmax * B) ) / (Km + B))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] NAD+ + Prostaglandin_E2 -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+ + NADH	`(( (Vmax * B) ) / (Km + B))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:O08699 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] NAD+ + Prostaglandin_E2 -> NADH + H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:O08699 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] NAD+ + Prostaglandin_E2 -> NADH + H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (Vmax * B) ) / (Km + B))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:O08699 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant chimeric 1-252_rat/253-266_human (P15428)] NAD+ + Prostaglandin_E2 -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant 255, C-terminal 11 residues deleted] Prostaglandin_E2_methyl_ester + NAD+ -> NADH + 15_Keto_prostaglandin_E2_methyl_ester + H+	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant 255, C-terminal 11 residues deleted] NAD+ + Prostaglandin_E2 -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant 259, C-terminal 7 residues deleted] Prostaglandin_E2 + NAD+ -> NADH + H+ + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant 263, C-terminal 3 residues deleted] NAD+ + Prostaglandin_E2 -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant 263, C-terminal 3 residues deleted] Prostaglandin_E2 + NAD+ -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+	`(( (Vmax * B) ) / (Km + B))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant 259, C-terminal 7 residues deleted] Prostaglandin_E2 + NAD+ -> (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + NADH + H+	`(( (Vmax * B) ) / (Km + B))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:Q8VCC1 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] NAD+ + Prostaglandin_E2 -> NADH + (5Z,13E)_11alpha_Hydroxy_9,15_dioxoprost_13_enoate + H+	`(( (Vmax * B) ) / (Km + B))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant 263, C-terminal 3 residues deleted] Prostaglandin_E2_methyl_ester + NAD+ -> H+ + NADH + 15_Keto_prostaglandin_E2_methyl_ester	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) wildtype] Prostaglandin_E2_methyl_ester + NAD+ -> H+ + 15_Keto_prostaglandin_E2_methyl_ester + NADH	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37
uniprot:P15428 [15-hydroxyprostaglandin dehydrogenase (NAD+)(Enzyme) mutant 259, C-terminal 7 residues deleted] Prostaglandin_E2_methyl_ester + NAD+ -> H+ + 15_Keto_prostaglandin_E2_methyl_ester + NADH	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris/HCl, 0.1 mM dithiothreitol PH: 7.5 Temperature: 37

Pathways

pathway id	name
BioCyc:MOUSE_PWY3DJ-35583	biosynthesis of prostaglandins
Reactome:R-HSA-9018896	Biosynthesis of E-series 18(S)-resolvins
Reactome:R-MMU-1430728	Metabolism
Reactome:R-MMU-556833	Metabolism of lipids
Reactome:R-MMU-9018679	Biosynthesis of EPA-derived SPMs
Reactome:R-MMU-9018676	Biosynthesis of D-series resolvins
Reactome:R-RNO-2142700	Biosynthesis of Lipoxins (LX)
Reactome:R-RNO-9018677	Biosynthesis of DHA-derived SPMs
Reactome:R-HSA-556833	Metabolism of lipids
Reactome:R-HSA-8978868	Fatty acid metabolism
Reactome:R-HSA-2162123	Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-HSA-9018679	Biosynthesis of EPA-derived SPMs
Reactome:R-HSA-9018676	Biosynthesis of D-series resolvins
Reactome:R-MMU-2142700	Biosynthesis of Lipoxins (LX)
Reactome:R-MMU-9018677	Biosynthesis of DHA-derived SPMs
Reactome:R-RNO-9018678	Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-HSA-9018678	Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-MMU-9018896	Biosynthesis of E-series 18(S)-resolvins
Reactome:R-RNO-1430728	Metabolism
Reactome:R-RNO-556833	Metabolism of lipids
Reactome:R-RNO-9018679	Biosynthesis of EPA-derived SPMs
Reactome:R-RNO-9018676	Biosynthesis of D-series resolvins
Reactome:R-HSA-1430728	Metabolism
Reactome:R-HSA-2142753	Arachidonate metabolism
Reactome:R-HSA-2142700	Biosynthesis of Lipoxins (LX)
Reactome:R-HSA-9018677	Biosynthesis of DHA-derived SPMs
Reactome:R-MMU-9018678	Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-RNO-9018896	Biosynthesis of E-series 18(S)-resolvins

数据之源，洞见之始

Core Resources

Data & Analytics

Sophia Virtual Cell Workshop 2026

EC: 1.1.1.141

Pathways