uniprot:P29728 [2'-5' oligoadenylate synthase(Enzyme) wildtype His6-tagged]
dATP + P1,P3_Bis(5'_adenosyl)_triphosphate -> ApppA2'p5'A + Diphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-Cl, 20 mM magnesium acetate, 2.5 mM dithiothreitol
PH: 7
Temperature: 30 |
uniprot:P29728 [2'-5' oligoadenylate synthase(Enzyme) mutant heterodimer wt His6-tagged/DADA (D408A,D410A) FLAG-tagged]
dATP + P1,P3_Bis(5'_adenosyl)_triphosphate -> Diphosphate + ApppA2'p5'A |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-Cl, 20 mM magnesium acetate, 2.5 mM dithiothreitol
PH: 7
Temperature: 30 |
uniprot:P29728 [2'-5' oligoadenylate synthase(Enzyme) mutant heterodimer wt His6-tagged/DADA (D408A,D410A) FLAG-tagged]
3 ATP -> 2 Diphosphate + pppA2'p5'A2'p5'A |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-Cl, 20 mM magnesium acetate, 2.5 mM dithiothreitol
PH: 7
Temperature: 30 |
uniprot:P29728 [2'-5' oligoadenylate synthase(Enzyme) wildtype His6-tagged]
3 ATP -> 2 Diphosphate + pppA2'p5'A2'p5'A |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-Cl, 20 mM magnesium acetate, 2.5 mM dithiothreitol
PH: 7
Temperature: 30 |
uniprot:P29728 [dsRNA]
3 ATP -> 2 Diphosphate + pppA2'p5'A2'p5'A |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-Cl, 2.5 mM dithiothreitol
PH: 7.4
Temperature: 30 |
uniprot:Q9Z2F2 [dsRNA]
3 ATP -> 2 Diphosphate + pppA2'p5'A2'p5'A |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 75 mM Mg(OAc)2, 1 mM dithiothreitol, 0.2 mM EDTA, 0.5 mg/ml bovine serum albumin, 10% (v/v) glycerol, 30 mM creatine phosphate, 0.5 mg/ml creatine kinase
PH: 7.5
Temperature: 37 |
uniprot:Q9Z2F2 [dsRNA]
3 ATP -> 2 Diphosphate + pppA2'p5'A2'p5'A |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 75 mM Mg(OAc)2, 1 mM dithiothreitol, 0.2 mM EDTA, 0.5 mg/ml bovine serum albumin, 10% (v/v) glycerol, 30 mM creatine phosphate, 0.5 mg/ml creatine kinase
PH: 7.5
Temperature: 37 |
uniprot:P00973 [dsRNA]
3 ATP -> pppA2'p5'A2'p5'A + 2 Diphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 75 mM Mg(OAc)2, 1 mM dithiothreitol, 0.2 mM EDTA, 0.5 mg/ml bovine serum albumin, 10% (v/v) glycerol, 30 mM creatine phosphate, 0.5 mg/ml creatine kinase
PH: 7.5
Temperature: 37 |
uniprot:P00973 [dsRNA]
3 ATP -> pppA2'p5'A2'p5'A + 2 Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 75 mM Mg(OAc)2, 1 mM dithiothreitol, 0.2 mM EDTA, 0.5 mg/ml bovine serum albumin, 10% (v/v) glycerol, 30 mM creatine phosphate, 0.5 mg/ml creatine kinase
PH: 7.5
Temperature: 37 |
uniprot:P00973 [dsRNA]
3 ATP -> pppA2'p5'A2'p5'A + 2 Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 75 mM Mg(OAc)2, 1 mM dithiothreitol, 0.2 mM EDTA, 0.5 mg/ml bovine serum albumin, 10% (v/v) glycerol, 30 mM creatine phosphate, 0.5 mg/ml creatine kinase
PH: 7.5
Temperature: 37 |