EC: 2.7.1.23

NAD+ kinase (ATP:NAD+ 2'-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q58327 [Mg2+]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8.5
Temperature: 85
uniprot:Q58327 [Mg2+]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8.5
Temperature: 85
uniprot:Q4G0N4 [NAD+ kinase(Enzyme) wildtype]

ATP + NAD+ -> ADP + NADP+		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 5 mM glucose 6-phosphate, 0.5 U/ml glucose 6-phosphate dehydrogenase, 40 mM MgCl2
PH: 8
Temperature: 37
uniprot:Q4G0N4 [NAD+ kinase(Enzyme) wildtype]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM glucose 6-phosphate, 0.5 U/ml glucose 6-phosphate dehydrogenase, 20 mM MgCl2
PH: 8
Temperature: 37
uniprot:Q4G0N4 [NAD+ kinase(Enzyme) wildtype]

NAD+ + Tetrapolyphosphate -> NADP+ + Triphosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 5 mM glucose 6-phosphate, 0.5 U/ml glucose 6-phosphate dehydrogenase, 5 mM MgCl2
PH: 8
Temperature: 37
uniprot:P65774 [NAD+ kinase(Enzyme) wildtype]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.5
Temperature: 37
uniprot:P65774 [NAD+ kinase(Enzyme) wildtype C-terminal His-tagged]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.5
Temperature: 37
uniprot:P65774 [NAD+ kinase(Enzyme) wildtype C-terminal His-tagged]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.5
Temperature: 37
uniprot:P65774 [NAD+ kinase(Enzyme) wildtype]

ATP + NAD+ -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.5
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant S199A]

ATP + NAD+ -> NADP+ + ADP		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 50 mM Tris, 20 mM MgCl2
PH: 8
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant T195A]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 50 mM Tris, 20 mM MgCl2
PH: 8
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) wildtype]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 50 mM Tris, 20 mM MgCl2
PH: 8
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) wildtype]

ATP + NAD+ -> ADP + NADP+		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 50 mM Tris, 20 mM MgCl2
PH: 8
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant G208A]

ATP + NAD+ -> ADP + NADP+		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 50 mM Tris, 20 mM MgCl2
PH: 8
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant G208A]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 50 mM Tris, 20 mM MgCl2
PH: 8
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant T195A]

ATP + NAD+ -> ADP + NADP+		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 50 mM Tris, 20 mM MgCl2
PH: 8
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant S199A]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 50 mM Tris, 20 mM MgCl2
PH: 8
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) wildtype]

NAD+ + n_1_n_n_1_n_Polyphosphate -> NADP+ + n_1_n_n_1_n_Polyphosphate		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Pipes, 20 mM MgCl2
PH: 6.5
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant T195A]

NAD+ + n_1_n_n_1_n_Polyphosphate -> NADP+ + n_1_n_n_1_n_Polyphosphate		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Pipes, 20 mM MgCl2
PH: 6.5
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant S199A]

NAD+ + n_1_n_n_1_n_Polyphosphate -> NADP+ + n_1_n_n_1_n_Polyphosphate		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Pipes, 20 mM MgCl2
PH: 6.5
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant G208A]

NAD+ + n_1_n_n_1_n_Polyphosphate -> n_1_n_n_1_n_Polyphosphate + NADP+		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Pipes, 20 mM MgCl2
PH: 6.5
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) wildtype]

n_1_n_n_1_n_Polyphosphate + NAD+ -> n_1_n_n_1_n_Polyphosphate + NADP+		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Pipes, 20 mM MgCl2
PH: 6.5
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant T195A]

n_1_n_n_1_n_Polyphosphate + NAD+ -> NADP+ + n_1_n_n_1_n_Polyphosphate		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Pipes, 20 mM MgCl2
PH: 6.5
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant T195A]

n_1_n_n_1_n_Polyphosphate + NAD+ -> NADP+ + n_1_n_n_1_n_Polyphosphate		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Pipes, 20 mM MgCl2
PH: 6.5
Temperature: 37
uniprot:P0A5S6 [NAD+ kinase(Enzyme) mutant G208A]

NAD+ + n_1_n_n_1_n_Polyphosphate -> n_1_n_n_1_n_Polyphosphate + NADP+		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Pipes, 20 mM MgCl2
PH: 6.5
Temperature: 37
uniprot:O95544 [Mg2+]

NAD+ + ATP -> ADP + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 100 mM KCl, 100 mM NaCl, 1 mM Glucose 6-phosphate, 1 U Glucose 6-phosphate dehydrogenase
PH: 7.8
Temperature: 30
uniprot:O95544 [Mg2+]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 100 mM KCl, 100 mM NaCl, 1 mM Glucose 6-phosphate, 1 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.8
Temperature: 30
uniprot:Q8VUL9 [NAD+ kinase(Enzyme) mutant G183R]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:Q8VUL9 [NAD+ kinase(Enzyme) mutant G183R]

ATP + NAD+ -> ADP + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:Q8VUL9 [NAD+ kinase(Enzyme) mutant G183R]

NAD+ + Tetrapolyphosphate -> Triphosphate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:Q8VUL9 [NAD+ kinase(Enzyme) mutant G183R]

NAD+ + Tetrapolyphosphate -> NADP+ + Triphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:P0A7B3 [NAD+ kinase(Enzyme) wildtype]

NAD+ + ATP -> ADP + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:P0A7B3 [NAD+ kinase(Enzyme) mutant R175G]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:P0A7B3 [NAD+ kinase(Enzyme) wildtype]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:P0A7B3 [NAD+ kinase(Enzyme) mutant R175G]

NAD+ + ATP -> NADP+ + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:Q8VUL9 [NAD+ kinase(Enzyme) wildtype]

NAD+ + ATP -> ADP + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:Q8VUL9 [NAD+ kinase(Enzyme) wildtype]

NAD+ + ATP -> ADP + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:Q8VUL9 [NAD+ kinase(Enzyme) wildtype]

Tetrapolyphosphate + NAD+ -> Triphosphate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:Q8VUL9 [NAD+ kinase(Enzyme) wildtype]

Tetrapolyphosphate + NAD+ -> Triphosphate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5.0 mM MgCl2, glucose 6-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:P0A7B3 [Mg2+]

NAD+ + ATP -> NADP+ + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7
Temperature: 37
uniprot:P0A7B3 [Mg2+]

ATP + NAD+ -> NADP+ + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7
Temperature: 37
uniprot:P0A7B3 [Mg2+]

ATP + NAD+ -> ADP + NADP+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl
PH: 7
Temperature: 37
uniprot:P0A7B3 [Mg2+]

ATP + NAD+ -> ADP + NADP+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl
PH: 7
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_NADPHOS-DEPHOS-PWY-1 NAD phosphorylation and dephosphorylation
BioCyc:META_NADPHOS-DEPHOS-PWY-1 NAD phosphorylation and transhydrogenation
BioCyc:META_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:YEAST_PWY-7269 NAD/NADP-NADH/NADPH mitochondrial interconversion (yeast)
BioCyc:VCHO_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:MTBH37RV_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:ECOL316407_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation I
BioCyc:META_PWY-7268 NAD/NADP-NADH/NADPH cytosolic interconversion (yeast)
BioCyc:MOUSE_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:BSUB_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:YEAST_PWY-7245 superpathway NAD/NADP - NADH/NADPH interconversion
BioCyc:GCF_000013425_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:META_PWY-7245 superpathway NAD/NADP - NADH/NADPH interconversion (yeast)
BioCyc:ECO_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:YEAST_PWY-7268 NAD/NADP-NADH/NADPH cytosolic interconversion
BioCyc:VCHO_NADPHOS-DEPHOS-PWY-1 NAD phosphorylation and transhydrogenation
BioCyc:MTBH37RV_NADPHOS-DEPHOS-PWY-1 NAD phosphorylation and transhydrogenation
BioCyc:CORYNE_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
PathBank:SMP0002093 NAD Phosphorylation and Dephosphorylation
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SCE-196807 Nicotinate metabolism
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-XTR-196807 Nicotinate metabolism
Reactome:R-HSA-196807 Nicotinate metabolism
Reactome:R-MMU-196807 Nicotinate metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-196807 Nicotinate metabolism
Reactome:R-XTR-1430728 Metabolism
Reactome:R-XTR-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-XTR-196849 Metabolism of water-soluble vitamins and cofactors
PathBank:SMP0083297 NAD+ Signalling Pathway (Cancer)
PathBank:SMP0084271 NAD+ Signalling and Aging
PathBank:SMP0000048 Nicotinate and Nicotinamide Metabolism