EC: 2.2.1.9

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (isochorismate:2-oxoglutarate 4-oxopentanoatetransferase (decarboxylating))

enzyme lambda metabolic reaction experiment
uniprot:P17109 [Mn2+]

Isochorismate + 2_Oxoglutarate -> CO2 + 2_Succinyl_6_hydroxy_2,4_cyclohexadiene_1_carboxylate + Pyruvate		 (( (Vmax * (S ^ h)) ) / (( (S_h ^ h) ) + (S ^ h))) buffer: 100 mM Tris-HCl, 100 mM KCl, 1 mM ditiothreitol, 2 µl (of 12.5 unit/µl) rabbit muscle LDH, 40 µM NADH
PH: 8.5
Temperature: 25
uniprot:P17109 [Mn2+]

2_Oxoglutarate + Isochorismate -> CO2 + 2_Succinyl_6_hydroxy_2,4_cyclohexadiene_1_carboxylate + Pyruvate		 (( (Vmax * (S ^ h)) ) / (( (S_h ^ h) ) + (S ^ h))) buffer: 100 mM Tris-HCl, 100 mM KCl, 1 mM ditiothreitol, 2 µl (of 12.5 unit/µl) rabbit muscle LDH, 40 µM NADH
PH: 8.5
Temperature: 25
uniprot:P17109 [Enzyme wildtype MenD]

2_Oxoglutarate + Isochorismate -> 2_Succinyl_6_hydroxy_2,4_cyclohexadiene_1_carboxylate + Pyruvate + CO2		 (( (Vmax * (S ^ h)) ) / (( (S_h ^ h) ) + (S ^ h))) buffer: 100 mM Tris-HCl, 100 mM KCl, 1 mM ditiothreitol, 2 µl (of 12.5 unit/µl) rabbit muscle LDH, 40 µM NADH
PH: 8.5
Temperature: 25
uniprot:P17109 [Mn2+]

Isochorismate + 2_Oxoglutarate -> CO2 + Pyruvate + 2_Succinyl_6_hydroxy_2,4_cyclohexadiene_1_carboxylate		 (( (Vmax * (S ^ h)) ) / (( (S_h ^ h) ) + (S ^ h))) buffer: 100 mM Tris-HCl, 100 mM KCl, 1 mM ditiothreitol, 2 µl (of 12.5 unit/µl) rabbit muscle LDH, 40 µM NADH
PH: 8.5
Temperature: 25

Pathways

pathway id name
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-5862 superpathway of demethylmenaquinol-9 biosynthesis
BioCyc:ECO_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
BioCyc:SYNEL_P105-PWY TCA cycle IV (2-oxoglutarate decarboxylase)
BioCyc:ECOL316407_PWY-5837 1,4-dihydroxy-2-naphthoate biosynthesis I
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:BTHE_PWY-5084 2-oxoglutarate decarboxylation to succinyl-CoA
BioCyc:META_PWY-5861 superpathway of demethylmenaquinol-8 biosynthesis I
BioCyc:META_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
BioCyc:META_PWY-5838 superpathway of menaquinol-8 biosynthesis I
BioCyc:ARA_PWY-5791 1,4-dihydroxy-2-naphthoate biosynthesis II (plants)
BioCyc:VCHO_PWY-5861 superpathway of demethylmenaquinol-8 biosynthesis
BioCyc:VCHO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:GCF_000013425_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
BioCyc:META_PWY-5863 superpathway of phylloquinol biosynthesis
BioCyc:VCHO_PWY-5838 superpathway of menaquinol-8 biosynthesis I
BioCyc:MTBH37RV_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
BioCyc:MTBCDC1551_PWY-5837 1,4-dihydroxy-2-naphthoate biosynthesis
BioCyc:ANTHRA_PWY-5840 superpathway of menaquinol-7 biosynthesis
BioCyc:ANTHRA_PWY-5837 1,4-dihydroxy-2-naphthoate biosynthesis
BioCyc:BSUB_PWY-5840 superpathway of menaquinol-7 biosynthesis
BioCyc:SYNEL_PWY-5863 superpathway of phylloquinol biosynthesis
BioCyc:SYNEL_PWY-5837 1,4-dihydroxy-2-naphthoate biosynthesis
BioCyc:SYNEL_PWY-5084 2-oxoglutarate decarboxylation to succinyl-CoA
BioCyc:META_PWY-5840 superpathway of menaquinol-7 biosynthesis
BioCyc:ECO_PWY-5838 superpathway of menaquinol-8 biosynthesis I
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ARA_PWY-5837 1,4-dihydroxy-2-naphthoate biosynthesis
BioCyc:VCHO_PWY-5837 1,4-dihydroxy-2-naphthoate biosynthesis
BioCyc:MTBCDC1551_PWY-5845 superpathway of menaquinol-9 biosynthesis
BioCyc:MTBCDC1551_PWY-5862 superpathway of demethylmenaquinol-9 biosynthesis
BioCyc:ECOO157_PWY-5837 1,4-dihydroxy-2-naphthoate biosynthesis I
BioCyc:ANTHRA_PWY-5861 superpathway of demethylmenaquinol-8 biosynthesis
BioCyc:ANTHRA_PWY-5838 superpathway of menaquinol-8 biosynthesis I
BioCyc:BSUB_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
BioCyc:ECOL316407_PWY-5838 superpathway of menaquinol-8 biosynthesis I
PathBank:SMP0001911 Menaquinol Biosythesis
PlantCyc:ARA_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
PlantCyc:ARA_PWY-5791 1,4-dihydroxy-2-naphthoate biosynthesis II (plants)
PlantCyc:PLANT_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
PlantCyc:ARA_PWY-5863 superpathway of phylloquinol biosynthesis
PlantCyc:PLANT_PWY-5791 1,4-dihydroxy-2-naphthoate biosynthesis II (plants)
PlantCyc:PLANT_PWY-5863 superpathway of phylloquinol biosynthesis