EC: 2.1.1.100

protein-S-isoprenylcysteine O-methyltransferase (S-adenosyl-L-methionine:protein-C-terminal-S-farnesyl-L-cysteine O-methyltransferase)

enzyme lambda metabolic reaction experiment
- [all-trans-Farnesylthioacetic acid]

N_Acetyl_S_trans,trans_farnesyl_L_cysteine + S_Adenosyl_L_methionine -> N_Acetyl_S_trans,trans_farnesyl_L_cysteine_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 200 mM Hepes, 100 mM NaCl, 5 mM MgCl2, 8% (v/v) Dimethyl sulfoxide
PH: 7.4
Temperature: 37
uniprot:O60725 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

N_Acetyl_S_trans,trans_farnesyl_L_cysteine + S_Adenosyl_L_methionine -> N_Acetyl_S_trans,trans_farnesyl_L_cysteine_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM NaEDTA
PH: 8
Temperature: 37
- [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine -> N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Hepes, 100 mM NaCl, 4 mM MgCl2, 4% (v/v) Dimethyl sulfoxide
PH: 7.4
Temperature: 37
- [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

N_Acetyl_S_trans,trans_farnesyl_L_cysteine + S_Adenosyl_L_methionine -> N_Acetyl_S_trans,trans_farnesyl_L_cysteine_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Hepes, 100 mM NaCl, 4 mM MgCl2, 4% (v/v) Dimethyl sulfoxide
PH: 7.4
Temperature: 37
uniprot:Q9FMW9 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype AtSTE14A]

S_Adenosyl_L_methionine + N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine -> S_Adenosyl_L_homocysteine + N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine_methyl_ester		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES
PH: 7
Temperature: 30
uniprot:Q93W54 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype AtSTE14B]

S_Adenosyl_L_methionine + N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine -> S_Adenosyl_L_homocysteine + N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine_methyl_ester		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES
PH: 7
Temperature: 30
uniprot:Q9FMW9 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype AtSTE14A]

S_Adenosyl_L_methionine + N_Acetyl_S_trans,trans_farnesyl_L_cysteine -> N_Acetyl_S_trans,trans_farnesyl_L_cysteine_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES
PH: 7
Temperature: 30
uniprot:Q93W54 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype AtSTE14B]

S_Adenosyl_L_methionine + N_Acetyl_S_trans,trans_farnesyl_L_cysteine -> S_Adenosyl_L_homocysteine + N_Acetyl_S_trans,trans_farnesyl_L_cysteine_methyl_ester		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES
PH: 7
Temperature: 30
uniprot:O60725 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine_methyl_ester		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM NaEDTA
PH: 8
Temperature: 37
uniprot:O60725 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

N_Acetyl_S_trans,trans_farnesyl_L_cysteine + S_Adenosyl_L_methionine -> N_Acetyl_S_trans,trans_farnesyl_L_cysteine_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM NaEDTA
PH: 8
Temperature: 37
- [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine_methyl_ester		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Hepes, 100 mM NaCl, 4 mM MgCl2, 4% (v/v) Dimethyl sulfoxide
PH: 7.4
Temperature: 37
- [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

N_Acetyl_S_trans,trans_farnesyl_L_cysteine + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + N_Acetyl_S_trans,trans_farnesyl_L_cysteine_methyl_ester		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Hepes, 100 mM NaCl, 4 mM MgCl2, 4% (v/v) Dimethyl sulfoxide
PH: 7.4
Temperature: 37
uniprot:Q9EQK7 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

N_Acetyl_S_trans,trans_farnesyl_L_cysteine + S_Adenosyl_L_methionine -> N_Acetyl_S_trans,trans_farnesyl_L_cysteine_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-Cl, 1 mM EDTA, 1 mM Dithiothreitol
PH: 7.9
Temperature: 37
uniprot:Q9EQK7 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + N_Acetyl_S_trans,trans_farnesyl_L_cysteine -> N_Acetyl_S_trans,trans_farnesyl_L_cysteine_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-Cl, 1 mM EDTA, 1 mM Dithiothreitol
PH: 7.9
Temperature: 37
uniprot:Q9WVM4 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + S_trans,trans_Farnesyl_Leu_Ala_Arg_Tyr_Lys_Cys -> S_Adenosyl_L_homocysteine + S_trans,trans_Farnesyl_Leu_Ala_Arg_Tyr_Lys_Cys_methyl_ester		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM HEPES, 60 mM NaCl
PH: 7.6
Temperature: 37
uniprot:Q9WVM4 [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + S_trans,trans_Farnesyl_Leu_Ala_Arg_Tyr_Lys_Cys -> S_trans,trans_Farnesyl_Leu_Ala_Arg_Tyr_Lys_Cys_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM HEPES, 60 mM NaCl
PH: 7.6
Temperature: 37
- [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine -> N_Acetyl_S_all_trans_geranylgeranyl_L_cysteine_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES
PH: 7
Temperature: 30
- [protein-S-isoprenylcysteine O-methyltransferase(Enzyme) wildtype]

N_Acetyl_S_trans,trans_farnesyl_L_cysteine + S_Adenosyl_L_methionine -> N_Acetyl_S_trans,trans_farnesyl_L_cysteine_methyl_ester + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES
PH: 7
Temperature: 30

Pathways

pathway id name
Reactome:R-RNO-9648002 RAS processing
Reactome:R-HSA-9648002 RAS processing
Reactome:R-MMU-9648002 RAS processing
Reactome:R-DDI-9648002 RAS processing
Reactome:R-DDI-597592 Post-translational protein modification
Reactome:R-DDI-5684996 ERK1/ERK2 pathway
Reactome:R-HSA-5683057 MAPK family signaling cascades
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-MMU-5673001 RAF/MAP kinase cascade
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-162582 Signaling Pathways
Reactome:R-SCE-597592 Post-translational protein modification
Reactome:R-DDI-392499 Metabolism of proteins
Reactome:R-DDI-5683057 MAPK family signaling cascades
Reactome:R-MMU-5684996 ERK1/ERK2 pathway
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-5673001 RAF/MAP kinase cascade
Reactome:R-SPO-163841 Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation
Reactome:R-DDI-163841 Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation
Reactome:R-DDI-5673001 RAF/MAP kinase cascade
Reactome:R-HSA-5684996 ERK1/ERK2 pathway
Reactome:R-HSA-163841 Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-RNO-163841 Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation
Reactome:R-RNO-5683057 MAPK family signaling cascades
Reactome:R-SCE-163841 Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation
Reactome:R-SPO-392499 Metabolism of proteins
Reactome:R-DDI-162582 Signaling Pathways
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-5673001 RAF/MAP kinase cascade
Reactome:R-MMU-5683057 MAPK family signaling cascades
Reactome:R-MMU-163841 Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation
Reactome:R-RNO-5684996 ERK1/ERK2 pathway
Reactome:R-SCE-392499 Metabolism of proteins
Reactome:R-SPO-597592 Post-translational protein modification
WikiPathways:WP4299 Lamin A-processing pathway
PathBank:SMP0121210 Terpenoid Backbone Biosynthesis