EC: 1.5.1.8

saccharopine dehydrogenase (NADP+, L-lysine-forming) (N6-(L-1,3-dicarboxypropyl)-L-lysine:NADP+ oxidoreductase (L-lysine-forming))

enzyme lambda metabolic reaction experiment
- [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype LKR/SDH]

2_Oxoglutarate + NADPH + L_Lysine + H+ -> NADP+ + H2O + N6_(L_1,3_Dicarboxypropyl)_L_lysine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl
PH: 7.2
Temperature: 30
- [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype LKR/SDH]

NADPH + L_Lysine + 2_Oxoglutarate + H+ -> NADP+ + N6_(L_1,3_Dicarboxypropyl)_L_lysine + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl
PH: 7.2
Temperature: 30
uniprot:Q9UDR5 [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype]

H+ + NADPH + 2_Oxoglutarate + L_Lysine -> NADP+ + H2O + N6_(L_1,3_Dicarboxypropyl)_L_lysine		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Hepes-NaOH
PH: 7.8
Temperature: 23
uniprot:Q9UDR5 [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype]

NADPH + 2_Oxoglutarate + H+ + L_Lysine -> H2O + NADP+ + N6_(L_1,3_Dicarboxypropyl)_L_lysine		 (( (Vmax * B) ) / (( (Km * (1 + (I / Ki))) ) + B)) buffer: 50 mM Hepes-NaOH
PH: 7.8
Temperature: 23
uniprot:Q9UDR5 [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype]

H+ + L_Lysine + NADPH + 2_Oxoglutarate -> NADP+ + H2O + N6_(L_1,3_Dicarboxypropyl)_L_lysine		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM Hepes-NaOH
PH: 7.8
Temperature: 23
uniprot:Q9UDR5 [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype]

2_Oxoglutarate + L_Lysine + NADPH + H+ -> NADP+ + H2O + N6_(L_1,3_Dicarboxypropyl)_L_lysine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes-NaOH
PH: 7.8
Temperature: 23
uniprot:Q9UDR5 [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype]

2_Oxoglutarate + NADPH + L_Lysine + H+ -> N6_(L_1,3_Dicarboxypropyl)_L_lysine + NADP+ + H2O		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes-NaOH
PH: 7.8
Temperature: 23
uniprot:Q9SMZ4 [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype AtLKR/SDHp, His-tagged]

L_Lysine + 2_Oxoglutarate + H+ + NADPH -> H2O + NADP+ + N6_(L_1,3_Dicarboxypropyl)_L_lysine		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 30
uniprot:Q9SMZ4 [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype AtLKR/SDHp, His-tagged]

H+ + L_Lysine + 2_Oxoglutarate + NADPH -> NADP+ + N6_(L_1,3_Dicarboxypropyl)_L_lysine + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 30
uniprot:Q9SMZ4 [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype AtLKR/SDHp, His-tagged]

H+ + NADPH + L_Lysine + 2_Oxoglutarate -> N6_(L_1,3_Dicarboxypropyl)_L_lysine + NADP+ + H2O		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 30
uniprot:O04709 [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype]

2_Oxoglutarate + H+ + L_Lysine + NADPH -> H2O + NADP+ + N6_(L_1,3_Dicarboxypropyl)_L_lysine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 30
uniprot:O04709 [saccharopine dehydrogenase (NADP+, L-lysine-forming)(Enzyme) wildtype]

2_Oxoglutarate + L_Lysine + H+ + NADPH -> NADP+ + N6_(L_1,3_Dicarboxypropyl)_L_lysine + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:META_LYSINE-DEG1-PWY L-lysine degradation XI (mammalian)
BioCyc:ARA_LYSINE-DEG2-PWY lysine degradation II
BioCyc:HUMAN_LYSINE-DEG1-PWY lysine degradation I (saccharopine pathway)
BioCyc:ARA_PWYQT-1 lysine degradation III
BioCyc:MOUSE_LYSINE-DEG1-PWY lysine degradation II
BioCyc:META_PWY-5327 superpathway of L-lysine degradation
PlantCyc:ARA_LYSINE-DEG2-PWY lysine degradation II
PlantCyc:PLANT_PWYQT-1 lysine degradation III
PlantCyc:ARA_PWYQT-1 lysine degradation III
PlantCyc:PLANT_LYSINE-DEG2-PWY lysine degradation II
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71064 Lysine catabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-71064 Lysine catabolism
Reactome:R-RNO-71064 Lysine catabolism
Reactome:R-BTA-71064 Lysine catabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
PathBank:SMP0120746 Saccharopinuria/Hyperlysinemia II
PathBank:SMP0120854 Pyridoxine Dependency with Seizures
PathBank:SMP0120870 2-Aminoadipic 2-Oxoadipic Aciduria
PathBank:SMP0000239 Saccharopinuria/Hyperlysinemia II
PathBank:SMP0000528 Hyperlysinemia II or Saccharopinuria
PathBank:SMP0000186 Glutaric Aciduria Type I
PathBank:SMP0000527 Hyperlysinemia I, Familial
PathBank:SMP0000571 Pyridoxine Dependency with Seizures
PathBank:SMP0120704 Glutaric Aciduria Type I
PathBank:SMP0120812 Hyperlysinemia II or Saccharopinuria
PathBank:SMP0000719 2-Aminoadipic 2-Oxoadipic Aciduria
PathBank:SMP0002434 Lysine Metabolism
PathBank:SMP0087203 Lysine Degradation
PathBank:SMP0087300 Lysine Degradation
PathBank:SMP0120811 Hyperlysinemia I, Familial
PathBank:SMP0000037 Lysine Degradation