choline biosynthesis I

Rontein D, Rhodes D, Hanson AD. Evidence from engineering that decarboxylation of free serine is the major source of ethanolamine moieties in plants. Plant Cell Physiol. 2003 Nov;44(11):1185–91. doi: 10.1093/pcp/pcg144. PMID: 14634155.; Mou Z, Wang X, Fu Z, Dai Y, Han C, Ouyang J, Bao F, Hu Y, Li J. Silencing of Phosphoethanolamine N-Methyltransferase Results in Temperature-Sensitive Male Sterility and Salt Hypersensitivity in Arabidopsis. Plant Cell. 2002 Aug 23;14(9):2031–43. doi: 10.1105/tpc.001701.; Rontein D, Nishida I, Tashiro G, Yoshioka K, Wu W, Voelker DR, Basset G, Hanson AD. Plants Synthesize Ethanolamine by Direct Decarboxylation of Serine Using a Pyridoxal Phosphate Enzyme. Journal of Biological Chemistry. 2001 Sep;276(38):35523–9. doi: 10.1074/jbc.m106038200.; McNeil SD, Nuccio ML, Ziemak MJ, Hanson AD. Enhanced synthesis of choline and glycine betaine in transgenic tobacco plants that overexpress phosphoethanolamine N -methyltransferase. Proc. Natl. Acad. Sci. U.S.A. 2001 Jul 31;98(17):10001–5. doi: 10.1073/pnas.171228998.; Nuccio ML, Ziemak MJ, Henry SA, Weretilnyk EA, Hanson AD. cDNA cloning of phosphoethanolamine N-methyltransferase from spinach by complementation in Schizosaccharomyces pombe and characterization of the recombinant enzyme. J Biol Chem. 2000 May 12;275(19):14095–101. doi: 10.1074/jbc.275.19.14095. PMID: 10799484.; McNeil SD, Nuccio ML, Rhodes D, Shachar-Hill Y, Hanson AD. Radiotracer and computer modeling evidence that phospho-base methylation is the main route of choline synthesis in tobacco. Plant Physiol. 2000 May;123(1):371–80. PMID: 10806254; PMCID: PMC59011.; Weretilnyk EA, Smith DD, Wilch GA, Summers PS. Enzymes of Choline Synthesis in Spinach (Response of Phospho-Base N-Methyltransferase Activities to Light and Salinity). Plant Physiol. 1995 Nov;109(3):1085–91. PMID: 12228655; PMCID: PMC161412.; Summers PS, Weretilnyk EA. Choline Synthesis in Spinach in Relation to Salt Stress. Plant Physiol. 1993 Dec;103(4):1269–76. PMID: 12232019; PMCID: PMC159115.

Metabolites

Choline

Formula: C5H14NO (104.10753340000001)

CAS ID: 62-49-7

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:



Enzyme

EC Number name full name note
4.1.1.-
2.1.1.103 phosphoethanolamine N-methyltransferase S-adenosyl-L-methionine:ethanolamine-phosphate N-methyltransferase
2.7.1.32 choline kinase ATP:choline phosphotransferase
2.7.1.82 ethanolamine kinase ATP:ethanolamine O-phosphotransferase
3.6.1.1 inorganic diphosphatase diphosphate phosphohydrolase


Proteins

Protein ID name full name