benzoate fermentation (to acetate and cyclohexane carboxylate)

Kung JW, Seifert J, von Bergen M, Boll M. Cyclohexanecarboxyl-Coenzyme A (CoA) and Cyclohex-1-ene-1-Carboxyl-CoA Dehydrogenases, Two Enzymes Involved in the Fermentation of Benzoate and Crotonate in Syntrophus aciditrophicus. Journal of Bacteriology. 2013 May 10;195(14):3193–200. doi: 10.1128/jb.00322-13.; Kung JW, Baumann S, von Bergen M, Müller M, Hagedoorn PL, Hagen WR, Boll M. Reversible biological Birch reduction at an extremely low redox potential. J Am Chem Soc. 2010 Jul 21;132(28):9850–6. doi: 10.1021/ja103448u. PMID: 20578740.; Boll M, Fuchs G. Benzoyl-coenzyme A reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. ATP dependence of the reaction, purification and some properties of the enzyme from Thauera aromatica strain K172. Eur J Biochem. 1995 Dec 15;234(3):921–33. doi: 10.1111/j.1432-1033.1995.921_a.x. PMID: 8575453.

Metabolites

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

benzoate

Formula: C7H5O2 (121.028953)

CAS ID: 766-76-7

acetate

Formula: C2H3O2 (59.0133038)

CAS ID: 71-50-1

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:



Enzyme

EC Number name full name note
1.3.8.10 cyclohex-1-ene-1-carbonyl-CoA dehydrogenase cyclohex-1-ene-1-carbonyl-CoA:electron transfer flavoprotein oxidoreductase
1.3.8.11 cyclohexane-1-carbonyl-CoA dehydrogenase (electron-transfer flavoprotein) cyclohexane-1-carbonyl-CoA:electron transfer flavoprotein oxidoreductase


Proteins

Protein ID name full name
Q2LQN9 CH1CO_SYNAS Cyclohex-1-ene-1-carbonyl-CoA dehydrogenase
Q2LQP0 CHCOA_SYNAS Cyclohexane-1-carbonyl-CoA dehydrogenase