Synthesis of substrates in N-glycan biosythesis

Sanyal S, Frank CG, Menon AK. Distinct flippases translocate glycerophospholipids and oligosaccharide diphosphate dolichols across the endoplasmic reticulum. Biochemistry. 2008 Jul 29;47(30):7937–46. PMID: 18597486; PMCID: PMC2646664.

Metabolites

D-Mannose

Formula: C6H12O6 (180.0633852)

CAS ID: 3458-28-4

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

GTP(4-)

Formula: C10H12N5O14P3 (518.9593642)

CAS ID: 86527-72-2

UDP-alpha-D-glucose(2-)

Formula: C15H22N2O17P2 (564.0393702)

CAS ID:

isopentenyl diphosphate(3-)

Formula: C5H9O7P2 (242.9823524)

CAS ID:

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
2.3.1.4 glucosamine-phosphate N-acetyltransferase acetyl-CoA:D-glucosamine-6-phosphate N-acetyltransferase
2.4.1.117 dolichyl-phosphate beta-glucosyltransferase UDP-glucose:dolichyl-phosphate beta-D-glucosyltransferase
2.4.1.83 dolichyl-phosphate beta-D-mannosyltransferase GDP-mannose:dolichyl-phosphate beta-D-mannosyltransferase
2.5.1.87 ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] (2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 10–55 isopentenyl units)
2.7.1.108 dolichol kinase CTP:dolichol O-phosphotransferase
2.7.7.13 mannose-1-phosphate guanylyltransferase GTP:alpha-D-mannose-1-phosphate guanylyltransferase
2.7.7.23 UDP-N-acetylglucosamine diphosphorylase UTP:N-acetyl-alpha-D-glucosamine-1-phosphate uridylyltransferase
3.1.2.22 palmitoyl[protein] hydrolase palmitoyl[protein] hydrolase
3.6.1.43 dolichyldiphosphatase dolichyl-diphosphate phosphohydrolase
4.1.1.33 diphosphomevalonate decarboxylase ATP:(R)-5-diphosphomevalonate carboxy-lyase (adding ATP; isopentenyl-diphosphate-forming)
5.3.1.8 mannose-6-phosphate isomerase D-mannose-6-phosphate aldose-ketose-isomerase
5.4.2.3 phosphoacetylglucosamine mutase N-Acetyl-alpha-D-glucosamine 1,6-phosphomutase
5.4.2.8 phosphomannomutase alpha-D-mannose 1,6-phosphomutase


Proteins

Protein ID name full name
O13738 gna1 Glucosamine 6-phosphate N-acetyltransferase
O13963 mvd1 Diphosphomevalonate decarboxylase
O14171 YE54_SCHPO Dehydrodolichyl diphosphate synthase complex subunit SPAC4D7.04c
O14466 dpm1 Dolichol-phosphate mannosyltransferase
O43014 pmi40 Mannose-6-phosphate isomerase
O59747 pdf1 Palmitoyl-protein thioesterase-dolichyl pyrophosphate phosphatase fusion 1
O60061 alg5 Dolichyl-phosphate beta-glucosyltransferase
O60064 YBB2_SCHPO Probable mannose-1-phosphate guanyltransferase
O74484 mpg1 Mannose-1-phosphate guanyltransferase
O94617 uap1 Probable UDP-N-acetylglucosamine pyrophosphorylase
Q09687 AGM1_SCHPO Phosphoacetylglucosamine mutase 1
Q9UTJ2 pmm1 Phosphomannomutase
Q9Y7K8 nus1 Dehydrodolichyl diphosphate synthase complex subunit nus1
Q9Y7T6 sec59 Dolichol kinase sec59