Synthesis of PIPs at the Golgi membrane

Sbrissa D, Ikonomov OC, Fenner H, Shisheva A. ArPIKfyve Homomeric and Heteromeric Interactions Scaffold PIKfyve and Sac3 in a Complex to Promote PIKfyve Activity and Functionality. Journal of Molecular Biology. 2008 Dec;384(4):766–79. doi: 10.1016/j.jmb.2008.10.009.; Sbrissa D, Ikonomov OC, Fu Z, Ijuin T, Gruenberg J, Takenawa T, Shisheva A. Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex. J Biol Chem. 2007 Aug 17;282(33):23878–91. doi: 10.1074/jbc.m611678200. PMID: 17556371.; Rohde HM, Cheong FY, Konrad G, Paiha K, Mayinger P, Boehmelt G. The Human Phosphatidylinositol Phosphatase SAC1 Interacts with the Coatomer I Complex. Journal of Biological Chemistry. 2003 Dec;278(52):52689–99. doi: 10.1074/jbc.m307983200.; Minogue S, Anderson JS, Waugh MG, dos Santos M, Corless S, Cramer R, Hsuan JJ. Cloning of a Human Type II Phosphatidylinositol 4-Kinase Reveals a Novel Lipid Kinase Family. Journal of Biological Chemistry. 2001 May;276(20):16635–40. doi: 10.1074/jbc.m100982200.; Arcaro A, Zvelebil MJ, Wallasch C, Ullrich A, Waterfield MD, Domin J. Class II Phosphoinositide 3-Kinases Are Downstream Targets of Activated Polypeptide Growth Factor Receptors. Molecular and Cellular Biology. 2000 Jun 01;20(11):3817–30. doi: 10.1128/mcb.20.11.3817-3830.2000.; Domin J, Gaidarov I, Smith ME, Keen JH, Waterfield MD. The class II phosphoinositide 3-kinase PI3K-C2alpha is concentrated in the trans-Golgi network and present in clathrin-coated vesicles. J Biol Chem. 2000 Apr 21;275(16):11943–50. doi: 10.1074/jbc.275.16.11943. PMID: 10766823.; Godi A, Pertile P, Meyers R, Marra P, Di Tullio G, Iurisci C, Luini A, Corda D, De Matteis MA. ARF mediates recruitment of PtdIns-4-OH kinase-beta and stimulates synthesis of PtdIns(4,5)P2 on the Golgi complex. Nat Cell Biol. 1999 Sep;1(5):280–7. doi: 10.1038/12993. PMID: 10559940.

Metabolites

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:



Enzyme

EC Number name full name note
2.7.1.67 1-phosphatidylinositol 4-kinase ATP:1-phosphatidyl-1D-myo-inositol 4-phosphotransferase
3.1.3.64 phosphatidylinositol-3-phosphatase 1-phosphatidyl-1D-myo-inositol-3-phosphate 3-phosphohydrolase
3.6.5.2 small monomeric GTPase GTP phosphohydrolase (cell-regulating)


Proteins

Protein ID name full name