Asparagine N-linked glycosylation

Lederkremer GZ. Glycoprotein folding, quality control and ER-associated degradation. Current Opinion in Structural Biology. 2009 Oct;19(5):515–23. doi: 10.1016/j.sbi.2009.06.004.; Shental-Bechor D, Levy Y. Folding of glycoproteins: toward understanding the biophysics of the glycosylation code. Current Opinion in Structural Biology. 2009 Oct;19(5):524–33. doi: 10.1016/j.sbi.2009.07.002.; Hossler P, Goh LT, Lee MM, Hu WS. GlycoVis: visualizing glycan distribution in the protein N-glycosylation pathway in mammalian cells. Biotechnol Bioeng. 2006 Dec 05;95(5):946–60. doi: 10.1002/bit.21062. PMID: 16807922.; Apweiler R, Hermjakob H, Sharon N. On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database. Biochim Biophys Acta. 1999 Dec 06;1473(1):4–8. doi: 10.1016/s0304-4165(99)00165-8. PMID: 10580125.

Metabolites

D-Mannose

Formula: C6H12O6 (180.0633852)

CAS ID: 3458-28-4

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Beta-1,4-D-Mannosylchitobiosyldiphosphodolichol

Formula: C47H82N2O22P2 (1088.4834212)

CAS ID:

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

GTP(4-)

Formula: C10H12N5O14P3 (518.9593642)

CAS ID: 86527-72-2

UDP-alpha-D-glucose(2-)

Formula: C15H22N2O17P2 (564.0393702)

CAS ID:

isopentenyl diphosphate(3-)

Formula: C5H9O7P2 (242.9823524)

CAS ID:

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
3.6.5.-
2.3.1.4 glucosamine-phosphate N-acetyltransferase acetyl-CoA:D-glucosamine-6-phosphate N-acetyltransferase
2.4.1.117 dolichyl-phosphate beta-glucosyltransferase UDP-glucose:dolichyl-phosphate beta-D-glucosyltransferase
2.4.1.132 GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase GDP-D-mannose:D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol 3-alpha-mannosyltransferase
2.4.1.141 N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase UDP-N-acetyl-D-glucosamine:N-acetyl-D-glucosaminyl-diphosphodolichol N-acetyl-D-glucosaminyltransferase
2.4.1.142 chitobiosyldiphosphodolichol beta-mannosyltransferase GDP-mannose:chitobiosyldiphosphodolichol beta-D-mannosyltransferase
2.4.1.257 GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase GDP-D-mannose:D-Man-alpha-(1->3)-D-Man-beta-(1-4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-6-mannosyltransferase
2.4.1.258 dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-mannosyltransferase
2.4.1.259 dolichyl-P-Man:Man6GlcNAc2-PP-dolichol alpha-1,2-mannosyltransferase dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,2-mannosyltransferase
2.4.1.260 dolichyl-P-Man:Man7GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,6-mannosyltransferase
2.4.1.261 dolichyl-P-Man:Man8GlcNAc2-PP-dolichol alpha-1,2-mannosyltransferase dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,2-mannosyltransferase
2.4.1.265 dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase dolichyl beta-D-glucosyl phosphate: D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-mannosyltransferase
2.4.1.267 dolichyl-P-Glc:Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase dolichyl beta-D-glucosyl phosphate: D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-mannosyltransferase
2.4.1.83 dolichyl-phosphate beta-D-mannosyltransferase GDP-mannose:dolichyl-phosphate beta-D-mannosyltransferase
2.5.1.87 ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] (2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 10–55 isopentenyl units)
2.7.1.108 dolichol kinase CTP:dolichol O-phosphotransferase
2.7.7.13 mannose-1-phosphate guanylyltransferase GTP:alpha-D-mannose-1-phosphate guanylyltransferase
2.7.7.23 UDP-N-acetylglucosamine diphosphorylase UTP:N-acetyl-alpha-D-glucosamine-1-phosphate uridylyltransferase
2.7.8.15 UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase UDP-N-acetyl-D-glucosamine:dolichyl-phosphate N-acetyl-D-glucosaminephosphotransferase
3.1.2.22 palmitoyl[protein] hydrolase palmitoyl[protein] hydrolase
3.6.1.43 dolichyldiphosphatase dolichyl-diphosphate phosphohydrolase
3.6.5.2 small monomeric GTPase GTP phosphohydrolase (cell-regulating)
4.1.1.33 diphosphomevalonate decarboxylase ATP:(R)-5-diphosphomevalonate carboxy-lyase (adding ATP; isopentenyl-diphosphate-forming)
5.3.1.8 mannose-6-phosphate isomerase D-mannose-6-phosphate aldose-ketose-isomerase
5.3.4.1 protein disulfide-isomerase Protein disulfide-isomerase
5.4.2.3 phosphoacetylglucosamine mutase N-Acetyl-alpha-D-glucosamine 1,6-phosphomutase
5.4.2.8 phosphomannomutase alpha-D-mannose 1,6-phosphomutase


Proteins

Protein ID name full name
O13738 gna1 Glucosamine 6-phosphate N-acetyltransferase
O13933 alg1 Chitobiosyldiphosphodolichol beta-mannosyltransferase
O13963 mvd1 Diphosphomevalonate decarboxylase
O14171 YE54_SCHPO Dehydrodolichyl diphosphate synthase complex subunit SPAC4D7.04c
O14190 alg13 UDP-N-acetylglucosamine transferase subunit alg13
O14466 dpm1 Dolichol-phosphate mannosyltransferase
O43014 pmi40 Mannose-6-phosphate isomerase
O43053 alg6 Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
O59747 pdf1 Palmitoyl-protein thioesterase-dolichyl pyrophosphate phosphatase fusion 1
O60061 alg5 Dolichyl-phosphate beta-glucosyltransferase
O60064 YBB2_SCHPO Probable mannose-1-phosphate guanyltransferase
O74484 mpg1 Mannose-1-phosphate guanyltransferase
O94617 uap1 Probable UDP-N-acetylglucosamine pyrophosphorylase
P36579 arf1 ADP-ribosylation factor 1
P42881 gpt2 UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Q01475 sar1 Small COPII coat GTPase sar1
Q09687 AGM1_SCHPO Phosphoacetylglucosamine mutase 1
Q10057 PDI1_SCHPO Putative protein disulfide-isomerase C1F5.02
Q10479 alg8 Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
Q96WW6 alg2 Alpha-1,3/1,6-mannosyltransferase alg2
Q9P7Q9 alg9 Alpha-1,2-mannosyltransferase alg9
Q9USD4 alg12 Probable Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Q9UTJ2 pmm1 Phosphomannomutase
Q9Y7I4 alg3 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Q9Y7K8 nus1 Dehydrodolichyl diphosphate synthase complex subunit nus1
Q9Y7T6 sec59 Dolichol kinase sec59