EC: 2.5.1.32

15-cis-phytoene synthase (geranylgeranyl-diphosphate:geranylgeranyl-diphosphate geranylgeranyltransferase (15-cis-phytoene forming))

enzyme lambda metabolic reaction experiment
uniprot:P37272 [Mn2+]

2 Geranylgeranyl_diphosphate -> Diphosphate + H+ + Prephytoene_diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM Dithiothreitol, 0.25 µg/µl Tween 80
PH: 7.6
Temperature: 30
uniprot:P37272 [Mn2+]

Prephytoene_diphosphate -> Phytoene + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM Dithiothreitol, 0.25 µg/µl Tween 80
PH: 7.6
Temperature: 30
uniprot:P37273 [ATP]

2 Geranylgeranyl_diphosphate -> Phytoene + 2 Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.4 M Tris-HCl, 1 mM DTT, 4 mM MgCl2, 0.1% (w/v) Tween 60, 1 mM KF
PH: 8
Temperature: 28
uniprot:P08196 [ATP]

2 Geranylgeranyl_diphosphate -> Phytoene + 2 Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.4 M Tris-HCl, 1 mM DTT, 4 mM MgCl2, 0.1% (w/v) Tween 60, 1 mM KF
PH: 8
Temperature: 28
uniprot:P21683 [ATP]

2 Geranylgeranyl_diphosphate -> Phytoene + 2 Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:ARA_PWY-6287 neurosporene biosynthesis
BioCyc:SYNEL_PWY-6475 trans-lycopene biosynthesis II (plants)
BioCyc:META_PWY-6681 neurosporaxanthin biosynthesis
BioCyc:META_CAROTENOID-PWY superpathway of carotenoid biosynthesis in plants
BioCyc:META_PWY-5943 β-carotene biosynthesis
BioCyc:ARA_PWY-6475 trans-lycopene biosynthesis II (plants)
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744344 Secondary metabolism
Plant Reactome:R-OSA-1119449 Carotenoid biosynthesis
PlantCyc:ARA_CAROTENOID-PWY superpathway of carotenoid biosynthesis in plants
PlantCyc:PLANT_PWY-7393 β-carotene biosynthesis (engineered)
PlantCyc:ARA_PWY-6287 neurosporene biosynthesis
PlantCyc:ARA_PWY-6475 trans-lycopene biosynthesis II (oxygenic phototrophs and green sulfur bacteria)
PlantCyc:PLANT_PWY-6475 trans-lycopene biosynthesis II (oxygenic phototrophs and green sulfur bacteria)