EC: 2.1.1.107

uroporphyrinogen-III C-methyltransferase (S-adenosyl-L-methionine:uroporphyrinogen-III C-methyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P29564 [uroporphyrinogen-III C-methyltransferase(Enzyme) wildtype]

2 S_Adenosyl_L_methionine + Uroporphyrinogen_III -> Precorrin_2 + 2 S_Adenosyl_L_homocysteine
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 5 mM DTT, 1 mM EDTA
PH: 7.7
Temperature: 30
- [uroporphyrinogen-III C-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + Uroporphyrinogen_III -> S_Adenosyl_L_homocysteine + Precorrin_1
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 5 mM DTT, 1 mM EDTA
PH: 7.7
Temperature: 30
- [uroporphyrinogen-III C-methyltransferase(Enzyme) wildtype]

Uroporphyrinogen_III + S_Adenosyl_L_methionine -> Precorrin_1 + S_Adenosyl_L_homocysteine
(( (Vmax * B) ) / (Km + B)) buffer: 0.1 M Tris-HCl, 5 mM DTT, 1 mM EDTA
PH: 7.7
Temperature: 30
- [uroporphyrinogen-III C-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + Uroporphyrinogen_III -> S_Adenosyl_L_homocysteine + Precorrin_1
(1 / (( (( (( (( (Km_A / Vmax) ) * (( (1 + (B / Kis_B)) ) + (( (Ki_A * Km_B) ) / (Km_A * B)))) ) * 1) ) / A) ) + (( (1 / Vmax) ) * (( (1 + (B / Kii_B)) ) + (Km_B / B))))) buffer: 0.1 M Tris-HCl, 5 mM DTT, 1 mM EDTA
PH: 7.7
Temperature: 30
- [uroporphyrinogen-III C-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + Uroporphyrinogen_III -> S_Adenosyl_L_homocysteine + Precorrin_1
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 5 mM DTT, 1 mM EDTA
PH: 7.7
Temperature: 30
- [uroporphyrinogen-III C-methyltransferase(Enzyme) wildtype]

Uroporphyrinogen_I + S_Adenosyl_L_methionine -> Methylated_uroporphyrinogen_I + S_Adenosyl_L_homocysteine
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.25 mM DTT, 1 mM EDTA
PH: 7.7
Temperature: 30
- [uroporphyrinogen-III C-methyltransferase(Enzyme) wildtype]

Heptacarboxyporphyrinogen_III + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Methylated_heptacarboxyporphyrinogen_III
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.25 mM DTT, 1 mM EDTA
PH: 7.7
Temperature: 30
- [uroporphyrinogen-III C-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + Pentacarboxyporphyrinogen_III -> Methylated_pentacarboxyporphyrinogen_III + S_Adenosyl_L_homocysteine
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.25 mM DTT, 1 mM EDTA
PH: 7.7
Temperature: 30
- [uroporphyrinogen-III C-methyltransferase(Enzyme) wildtype]

S_Adenosyl_L_methionine + Hexacarboxyporphyrinogen_III -> Methylated_hexacarboxyporphyrinogen_III + S_Adenosyl_L_homocysteine
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.25 mM DTT, 1 mM EDTA
PH: 7.7
Temperature: 30
- [uroporphyrinogen-III C-methyltransferase(Enzyme) wildtype]

Reduced_chlorin + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + Methylated_reduced_chlorin
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.25 mM DTT, 1 mM EDTA
PH: 7.7
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-5194 siroheme biosynthesis
BioCyc:ECO_PWY-5194 siroheme biosynthesis
BioCyc:META_PWY-5196 factor 430 biosynthesis
BioCyc:SYNEL_PWY-7376 cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)
BioCyc:SYNEL_P381-PWY adenosylcobalamin biosynthesis II (late cobalt incorporation)
BioCyc:META_PWY-7376 cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)
BioCyc:ARA_PWYQT-62 superpathway of proto- and siroheme biosynthesis
BioCyc:ECOO157_PWY-5194 siroheme biosynthesis
BioCyc:ECOL199310_PWY-7377 cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)
BioCyc:BSUB_PWY-5194 siroheme biosynthesis
BioCyc:SYNEL_PWY-5194 siroheme biosynthesis
BioCyc:META_PWY-7377 cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)
BioCyc:YEAST_PWY-5194 siroheme biosynthesis
BioCyc:ARA_PWY-5194 siroheme biosynthesis
BioCyc:ECOL199310_PWY-5194 siroheme biosynthesis
BioCyc:ECOL316407_PWY-5194 siroheme biosynthesis
PathBank:SMP0000953 Porphyrin Metabolism
PlantCyc:PLANT_PWY-5194 siroheme biosynthesis
PlantCyc:ARA_PWYQT-62 superpathway of proto- and siroheme biosynthesis
PlantCyc:ARA_PWY-5194 siroheme biosynthesis
PathBank:SMP0121321 Porphyrin Metabolism