cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)
Moore SJ, Biedendieck R, Lawrence AD, Deery E, Howard MJ, Rigby SEJ, Warren MJ. Characterization of the Enzyme CbiH60 Involved in Anaerobic Ring Contraction of the Cobalamin (Vitamin B12) Biosynthetic Pathway. Journal of Biological Chemistry. 2013 Jan;288(1):297–305. doi: 10.1074/jbc.m112.422535.; Roessner CA, Scott AI. Fine-Tuning Our Knowledge of the Anaerobic Route to Cobalamin (Vitamin B 12 ). J Bacteriol. 2006 Nov;188(21):7331–4. doi: 10.1128/jb.00918-06.; Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ. The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase. Nature Structural & Molecular Biology. 1998 Jul;5(7):585–92. doi: 10.1038/846.
Metabolites
Enzyme
| EC Number | name | full name | note |
|---|---|---|---|
| 2.1.1.- | |||
| 1.3.1.106 | cobalt-precorrin-6A reductase | cobalt-precorrin-6B:NAD+ oxidoreductase | |
| 1.3.1.76 | precorrin-2 dehydrogenase | precorrin-2:NAD+ oxidoreductase | |
| 2.1.1.107 | uroporphyrinogen-III C-methyltransferase | S-adenosyl-L-methionine:uroporphyrinogen-III C-methyltransferase | |
| 2.1.1.151 | cobalt-factor II C20-methyltransferase | S-adenosyl-L-methionine:cobalt-factor-II C20-methyltransferase | |
| 2.1.1.195 | cobalt-precorrin-5B (C1)-methyltransferase | S-adenosyl-L-methionine:cobalt-precorrin-5B (C1)-methyltransferase | |
| 2.1.1.196 | cobalt-precorrin-6B (C15)-methyltransferase [decarboxylating] | S-adenosyl-L-methionine:precorrin-6B C15-methyltransferase (C-12-decarboxylating) | |
| 2.1.1.271 | cobalt-precorrin-4 methyltransferase | S-adenosyl-L-methionine:cobalt-precorrin-4 11-methyltransferase | |
| 2.1.1.272 | cobalt-factor III methyltransferase | S-adenosyl-L-methionine:cobalt-factor III 17-methyltransferase (ring contracting) | |
| 2.1.1.289 | cobalt-precorrin-7 (C5)-methyltransferase | S-adenosyl-L-methionine:precorrin-7 C5-methyltransferase | |
| 3.7.1.12 | cobalt-precorrin 5A hydrolase | cobalt-precorrin 5A acylhydrolase | |
| 4.99.1.3 | sirohydrochlorin cobaltochelatase | cobalt-sirohydrochlorin cobalt-lyase (sirohydrochlorin-forming) | |
| 4.99.1.4 | sirohydrochlorin ferrochelatase | siroheme ferro-lyase (sirohydrochlorin-forming) | |
| 5.4.99.60 | cobalt-precorrin-8 methylmutase | precorrin-8 11,12-methylmutase | |
| 6.3.5.11 | cobyrinate a,c-diamide synthase | cobyrinate:L-glutamine amido-ligase (ADP-forming) |
Proteins
| Protein ID | name | full name |
|---|---|---|
| O87689 | cbiH60 | Cobalt-factor III methyltransferase |
| O87690 | cbiX | Sirohydrochlorin cobaltochelatase |
| O87691 | cbiJ | Cobalt-precorrin-6A reductase |
| O87692 | cbiC | Cobalt-precorrin-8 methylmutase |
| P0A2G9 | cbiF | Cobalt-precorrin-4 C(11)-methyltransferase |
| P0A2H1 | cbiE | Cobalt-precorrin-7 C(5)-methyltransferase |
| P25924 | cysG | Siroheme synthase |
| P29946 | cbiA | Cobyrinate a,c-diamide synthase |
| P61818 | sirC | Precorrin-2 dehydrogenase |
| Q05590 | cbiH | Probable cobalt-factor III C(17)-methyltransferase |
| Q05591 | cbiJ | Cobalt-precorrin-6A reductase |
| Q05592 | cbiK | Sirohydrochlorin cobaltochelatase |
| Q05593 | cbiL | Cobalt-precorrin-2 C(20)-methyltransferase |
| Q05601 | cbiC | Cobalt-precorrin-8 methylmutase |
| Q05628 | cbiD | Cobalt-precorrin-5B C(1)-methyltransferase |
| Q05631 | cbiG | Cobalt-precorrin-5A hydrolase |
| Q05632 | cbiT | Cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating) |