EC: 1.1.1.64

testosterone 17beta-dehydrogenase (NADP+) (17beta-hydroxysteroid:NADP+ 17-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P42330 [testosterone 17beta-dehydrogenase (NADP+)(Enzyme) wildtype AKR1C3]

Testosterone + NADP+ -> H+ + NADPH + Androst_4_ene_3,17_dione		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 25
uniprot:Q04828 [testosterone 17beta-dehydrogenase (NADP+)(Enzyme) wildtype AKR1C1]

NADP+ + Testosterone -> NADPH + Androst_4_ene_3,17_dione + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 25
uniprot:P42330 [EM-1404]

Testosterone + NADP+ -> NADPH + H+ + Androst_4_ene_3,17_dione		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer:
PH: 7.5
Temperature: 37
uniprot:Q3MHS3 [testosterone 17beta-dehydrogenase (NADP+)(Enzyme) wildtype]

Androst_4_ene_3,17_dione + H+ + NADPH -> Testosterone + NADP+		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 0.1 M Potassium phosphate
PH: 7.4
Temperature: 25
uniprot:Q3MHS3 [testosterone 17beta-dehydrogenase (NADP+)(Enzyme) wildtype]

NADP+ + Testosterone -> Androst_4_ene_3,17_dione + NADPH + H+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate
PH: 7.4
Temperature: 25
uniprot:Q3MHS3 [testosterone 17beta-dehydrogenase (NADP+)(Enzyme) wildtype]

Testosterone + NADP+ -> Androst_4_ene_3,17_dione + NADPH + H+		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 0.1 M Potassium phosphate
PH: 7.4
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-7455 allopregnanolone biosynthesis
BioCyc:HUMAN_PWY66-378 androgen biosynthesis
BioCyc:META_PWY66-378 androgen biosynthesis
BioCyc:META_PWY-7455 allopregnanolone biosynthesis
Reactome:R-HSA-5362517 Signaling by Retinoic Acid
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-HSA-193144 Estrogen biosynthesis
Reactome:R-HSA-9006931 Signaling by Nuclear Receptors
Reactome:R-HSA-5365859 RA biosynthesis pathway
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2142753 Arachidonate metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-196071 Metabolism of steroid hormones
Reactome:R-HSA-162582 Signaling Pathways
WikiPathways:WP5280 Glucocorticoid biosynthesis
WikiPathways:WP5368 Sulfatase and aromatase pathway
WikiPathways:WP5320 Male steroid hormones in cardiomyocyte energy metabolism
PathBank:SMP0120658 17-beta Hydroxysteroid Dehydrogenase III Deficiency
PathBank:SMP0030406 Androstenedione Metabolism
PathBank:SMP0000068 Androgen and Estrogen Metabolism
PathBank:SMP0000083 Acetylsalicylic Acid Action Pathway
PathBank:SMP0000087 Rofecoxib Action Pathway
PathBank:SMP0000094 Sulindac Action Pathway
PathBank:SMP0000104 Indomethacin Action Pathway
PathBank:SMP0000120 Naproxen Action Pathway
PathBank:SMP0000106 Meloxicam Action Pathway
PathBank:SMP0000693 Antrafenine Action Pathway
PathBank:SMP0000697 Flurbiprofen Action Pathway
PathBank:SMP0000701 Phenylbutazone Action Pathway
PathBank:SMP0000705 Tiaprofenic Acid Action Pathway
PathBank:SMP0000709 Salicylic Acid Action Pathway
PathBank:SMP0000075 Arachidonic Acid Metabolism
PathBank:SMP0000356 17-beta Hydroxysteroid Dehydrogenase III Deficiency
PathBank:SMP0000086 Ibuprofen Action Pathway
PathBank:SMP0000093 Diclofenac Action Pathway
PathBank:SMP0000098 Ketorolac Action Pathway
PathBank:SMP0000102 Bromfenac Action Pathway
PathBank:SMP0000114 Nabumetone Action Pathway
PathBank:SMP0000692 Antipyrine Action Pathway
PathBank:SMP0000696 Fenoprofen Action Pathway
PathBank:SMP0000700 Lornoxicam Action Pathway
PathBank:SMP0000704 Tolmetin Action Pathway
PathBank:SMP0000708 Salicylate-Sodium Action Pathway
PathBank:SMP0120848 Aromatase Deficiency
PathBank:SMP0000077 Piroxicam Action Pathway
PathBank:SMP0000084 Etodolac Action Pathway
PathBank:SMP0000109 Mefenamic Acid Action Pathway
PathBank:SMP0000289 Diflunisal Action Pathway
PathBank:SMP0000116 Valdecoxib Action Pathway
PathBank:SMP0000565 Aromatase Deficiency
PathBank:SMP0000650 Doxorubicin Metabolism Pathway
PathBank:SMP0000694 Carprofen Action Pathway
PathBank:SMP0000698 Magnesium Salicylate Action Pathway
PathBank:SMP0000702 Nepafenac Action Pathway
PathBank:SMP0000706 Tenoxicam Action Pathway
PathBank:SMP0000710 Acetaminophen Action Pathway
PathBank:SMP0087344 Androgen and Estrogen Metabolism
PathBank:SMP0087364 Androstenedione Metabolism
PathBank:SMP0000353 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000085 Ketoprofen Action Pathway
PathBank:SMP0000096 Celecoxib Action Pathway
PathBank:SMP0000101 Suprofen Action Pathway
PathBank:SMP0000113 Oxaprozin Action Pathway
PathBank:SMP0000695 Etoricoxib Action Pathway
PathBank:SMP0000699 Lumiracoxib Action Pathway
PathBank:SMP0000703 Trisalicylate-Choline Action Pathway
PathBank:SMP0000707 Salsalate Action Pathway