EC: 6.2.1.30

phenylacetate-CoA ligase (phenylacetate:CoA ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q9L9C1 [Mg2+]

Coenzyme_A + Phenylacetic_acid + ATP -> Phenylacetyl_CoA + Diphosphate + AMP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tris/HCl, 2 mM Dithioerythritol, 0.4 mM NADH, 1 mM Phosphoenolpyruvate, 17 nkatal/ml Myokinase, 17 nkatal/ml Pyruvate kinase, 25 nkatal/ml Lactate dehydrogenase
PH: 7.8
Temperature: 37
uniprot:Q9L9C1 [Mg2+]

ATP + Coenzyme_A + Phenylacetic_acid -> Phenylacetyl_CoA + AMP + Diphosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tris/HCl, 2 mM Dithioerythritol, 0.4 mM NADH, 1 mM Phosphoenolpyruvate, 17 nkatal/ml Myokinase, 17 nkatal/ml Pyruvate kinase, 25 nkatal/ml Lactate dehydrogenase
PH: 7.8
Temperature: 37
uniprot:Q9L9C1 [Mg2+]

ATP + Coenzyme_A + Phenylacetic_acid -> Diphosphate + AMP + Phenylacetyl_CoA		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tris/HCl, 2 mM Dithioerythritol, 0.4 mM NADH, 1 mM Phosphoenolpyruvate, 17 nkatal/ml Myokinase, 17 nkatal/ml Pyruvate kinase, 25 nkatal/ml Lactate dehydrogenase
PH: 7.8
Temperature: 37

Pathways

pathway id name
BioCyc:ECO_PWY0-321 phenylacetate degradation I (aerobic)
BioCyc:ECOL316407_PWY0-321 phenylacetate degradation I (aerobic)
BioCyc:META_PWY0-321 phenylacetate degradation I (aerobic)
BioCyc:ECO_PWY-6071 superpathway of phenylethylamine degradation
BioCyc:ECOL316407_PWY-6071 superpathway of phenylethylamine degradation
PathBank:SMP0002041 Phenylethylamine Metabolism