phenylacetate degradation I (aerobic)
Teufel R, Mascaraque V, Ismail W, Voss M, Perera J, Eisenreich W, Haehnel W, Fuchs G. Bacterial phenylalanine and phenylacetate catabolic pathway revealed. Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14390–5. PMID: 20660314; PMCID: PMC2922514.; Ismail W, El-Said Mohamed M, Wanner BL, Datsenko KA, Eisenreich W, Rohdich F, Bacher A, Fuchs G. Functional genomics by NMR spectroscopy. Phenylacetate catabolism in Escherichia coli. Eur J Biochem. 2003 Jul;270(14):3047–54. doi: 10.1046/j.1432-1033.2003.03683.x. PMID: 12846838.; Ferrández A, Miñambres B, García B, Olivera ER, Luengo JM, García JL, Díaz E. Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway. J Biol Chem. 1998 Oct 02;273(40):25974–86. doi: 10.1074/jbc.273.40.25974. PMID: 9748275.; Cooper RA, Jones DC, Parrott S. Isolation and mapping of Escherichia coli K12 mutants defective in phenylacetate degradation. J Gen Microbiol. 1985 Oct;131(10):2753–7. doi: 10.1099/00221287-131-10-2753. PMID: 3906029.
Metabolites
Enzyme
| EC Number | name | full name | note |
|---|---|---|---|
| 1.-.-.- | |||
| 1.14.13.149 | phenylacetyl-CoA 1,2-epoxidase | phenylacetyl-CoA:oxygen oxidoreductase (1,2-epoxidizing) | |
| 1.2.1.91 | 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase | 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde:NADP+ oxidoreductase | |
| 2.3.1.174 | 3-oxoadipyl-CoA thiolase | succinyl-CoA:acetyl-CoA C-succinyltransferase | |
| 2.3.1.223 | 3-oxo-5,6-didehydrosuberyl-CoA thiolase | 2,3-didehydroadipoyl-CoA:acetyl-CoA C-didehydroadipoyltransferase (double bond migration) | |
| 3.3.2.12 | oxepin-CoA hydrolase | 2-oxepin-2(3H)-ylideneacetyl-CoA hydrolyase | |
| 4.2.1.17 | enoyl-CoA hydratase | (3S)-3-hydroxyacyl-CoA hydro-lyase | |
| 5.3.3.18 | 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase | 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase | |
| 6.2.1.30 | phenylacetate-CoA ligase | phenylacetate:CoA ligase (AMP-forming) |
Proteins
| Protein ID | name | full name |
|---|---|---|
| P0C7L2 | paaJ | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase |
| P76077 | paaA | 1,2-phenylacetyl-CoA epoxidase, subunit A |
| P76081 | paaE | 1,2-phenylacetyl-CoA epoxidase, subunit E |
| P76082 | paaF | 2,3-dehydroadipyl-CoA hydratase |
| P76083 | paaH | 3-hydroxyadipyl-CoA dehydrogenase |
| P76085 | paaK | Phenylacetate-coenzyme A ligase |
| P77455 | paaZ | Bifunctional protein PaaZ |
| P77467 | paaG | 1,2-epoxyphenylacetyl-CoA isomerase |