EC: 5.5.1.6

chalcone isomerase (flavanone lyase (decyclizing))

enzyme lambda metabolic reaction experiment
uniprot:P28012 [chalcone isomerase(Enzyme) wildtype]

Isoliquiritigenin -> Liquiritigenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 5% (v/v) Ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) wildtype]

Naringenin_chalcone -> Naringenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 5% (v/v) Ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) wildtype]

2',4'_Dihydroxychalcone -> 7_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 5% (v/v) Ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) wildtype]

Isoliquiritigenin -> Liquiritigenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.05 M Hepes, 3% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant Y106F]

Isoliquiritigenin -> Liquiritigenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.05 M Hepes, 3% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T48S]

Naringenin_chalcone -> Naringenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T48A]

Naringenin_chalcone -> Naringenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant N113A]

Naringenin_chalcone -> Naringenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant Y106F]

Naringenin_chalcone -> Naringenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) wildtype]

4,2'_Dihydroxychalcone -> 4'_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 5% (v/v) Ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T48A]

Isoliquiritigenin -> Liquiritigenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant Y106F]

Isoliquiritigenin -> Liquiritigenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T48S]

Isoliquiritigenin -> Liquiritigenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant N113A]

Isoliquiritigenin -> Liquiritigenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T190A]

Isoliquiritigenin -> Liquiritigenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T190A]

Naringenin_chalcone -> Naringenin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant Y106F]

2',4'_Dihydroxychalcone -> 7_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T48S]

2',4'_Dihydroxychalcone -> 7_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T48A]

2',4'_Dihydroxychalcone -> 7_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant N113A]

4,2'_Dihydroxychalcone -> 4'_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T48S]

4,2'_Dihydroxychalcone -> 4'_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T48A]

4,2'_Dihydroxychalcone -> 4'_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T190A]

4,2'_Dihydroxychalcone -> 4'_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant Y106F]

4,2'_Dihydroxychalcone -> 4'_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant T190A]

2',4'_Dihydroxychalcone -> 7_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25
uniprot:P28012 [chalcone isomerase(Enzyme) mutant N113A]

2',4'_Dihydroxychalcone -> 7_Hydroxyflavanone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 5% (v/v) ethanol
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY1F-FLAVSYN flavonoid biosynthesis
BioCyc:META_PWY-7397 naringenin biosynthesis (engineered)
BioCyc:ARA_PWY-6787 flavonoid biosynthesis (in equisetum)
BioCyc:ARA_PWY1F-FLAVSYN flavonoid biosynthesis
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744344 Secondary metabolism
Plant Reactome:R-OSA-1119531 Flavonoid biosynthesis
PlantCyc:ARA_PWY-6787 flavonoid biosynthesis (in equisetum)
PlantCyc:PLANT_PWY1F-FLAVSYN flavonoid biosynthesis
PlantCyc:PLANT_PWY-7397 naringenin biosynthesis (engineered)
PlantCyc:ARA_PWY1F-FLAVSYN flavonoid biosynthesis
PlantCyc:LJAPONICUS_PWY-2002 isoflavonoid biosynthesis I
PlantCyc:LJAPONICUS_PWY-6325 echinatin biosynthesis
PlantCyc:PLANT_PWY-2002 isoflavonoid biosynthesis I
PathBank:SMP0012021 Flavonoid Biosynthesis
PathBank:SMP0012024 Flavanone Biosynthesis