EC: 5.4.99.18

5-(carboxyamino)imidazole ribonucleotide mutase (5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole carboxymutase)

enzyme lambda metabolic reaction experiment
uniprot:P0AG18 [5-(carboxyamino)imidazole ribonucleotide mutase(Enzyme) mutant H45N]

1_(5_Phospho_D_ribosyl)_5_amino_4_imidazolecarboxylate -> 5_carboxyamino_1_(5_phospho_D_ribosyl)imidazole		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 8
Temperature: 37
uniprot:P0AG18 [5-(carboxyamino)imidazole ribonucleotide mutase(Enzyme) mutant H45N]

5_carboxyamino_1_(5_phospho_D_ribosyl)imidazole -> 1_(5_Phospho_D_ribosyl)_5_amino_4_imidazolecarboxylate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 10 mM MgCl2, 2 mM PEP, 0.5 mM ATP, 10 mM L-aspartate, 2 U/ml pyruvate kinase, 4.2 U/ml EcPurK, 3.3 U/ml EcPurC, 37 μM AIR
PH: 7.5
Temperature: 23
uniprot:P0AG18 [5-(carboxyamino)imidazole ribonucleotide mutase(Enzyme) mutant H45W]

5_carboxyamino_1_(5_phospho_D_ribosyl)imidazole -> 1_(5_Phospho_D_ribosyl)_5_amino_4_imidazolecarboxylate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 10 mM MgCl2, 2 mM PEP, 0.5 mM ATP, 10 mM L-aspartate, 2 U/ml pyruvate kinase, 4.2 U/ml EcPurK, 3.3 U/ml EcPurC, 37 μM AIR
PH: 7.5
Temperature: 23
uniprot:P0AG18 [5-(carboxyamino)imidazole ribonucleotide mutase(Enzyme) wildtype]

1_(5_Phospho_D_ribosyl)_5_amino_4_imidazolecarboxylate -> 5_carboxyamino_1_(5_phospho_D_ribosyl)imidazole		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 8
Temperature: 37
uniprot:P0AG18 [5-(carboxyamino)imidazole ribonucleotide mutase(Enzyme) mutant H45Q]

1_(5_Phospho_D_ribosyl)_5_amino_4_imidazolecarboxylate -> 5_carboxyamino_1_(5_phospho_D_ribosyl)imidazole		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 8
Temperature: 37
uniprot:P0AG18 [5-(carboxyamino)imidazole ribonucleotide mutase(Enzyme) mutant H45W]

1_(5_Phospho_D_ribosyl)_5_amino_4_imidazolecarboxylate -> 5_carboxyamino_1_(5_phospho_D_ribosyl)imidazole		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 8
Temperature: 37
uniprot:P0AG18 [5-(carboxyamino)imidazole ribonucleotide mutase(Enzyme) wildtype]

5_carboxyamino_1_(5_phospho_D_ribosyl)imidazole -> 1_(5_Phospho_D_ribosyl)_5_amino_4_imidazolecarboxylate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 10 mM MgCl2, 2 mM PEP, 0.5 mM ATP, 10 mM L-aspartate, 2 U/ml pyruvate kinase, 4.2 U/ml EcPurK, 3.3 U/ml EcPurC, 37 μM AIR
PH: 7.5
Temperature: 23
uniprot:P0AG18 [5-(carboxyamino)imidazole ribonucleotide mutase(Enzyme) mutant H45Q]

5_carboxyamino_1_(5_phospho_D_ribosyl)imidazole -> 1_(5_Phospho_D_ribosyl)_5_amino_4_imidazolecarboxylate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 10 mM MgCl2, 2 mM PEP, 0.5 mM ATP, 10 mM L-aspartate, 2 U/ml pyruvate kinase, 4.2 U/ml EcPurK, 3.3 U/ml EcPurC, 37 μM AIR
PH: 7.5
Temperature: 23

Pathways

pathway id name
BioCyc:ECO_PWY-6123 inosine-5'-phosphate biosynthesis I
BioCyc:MTBCDC1551_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-6124 inosine-5'-phosphate biosynthesis II
BioCyc:VCHO_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:BSUB_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:BSUB_PWY-6124 inosine-5'-phosphate biosynthesis II
BioCyc:ECOL316407_PWY-6123 inosine-5'-phosphate biosynthesis I
BioCyc:META_PWY-6123 inosine-5'-phosphate biosynthesis I
BioCyc:VCHO_PWY-6124 inosine-5'-phosphate biosynthesis II
BioCyc:ECOO157_PWY-6123 inosine-5'-phosphate biosynthesis I
PathBank:SMP0000927 Purine Nucleotides De Novo Biosynthesis
PathBank:SMP0002047 Purine Nucleotides De Novo Biosynthesis 2