EC: 4.1.1.9

malonyl-CoA decarboxylase (malonyl-CoA carboxy-lyase (acetyl-CoA-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q920F5 [p-Chloromercuribenzoate]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 30 mM phosphate, 100 mM KCl, 75 mM sucrose
PH: 7
Temperature: 30
uniprot:Q920F5 [N-Ethylmaleimide]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 30 mM phosphate, 100 mM KCl, 75 mM sucrose
PH: 7
Temperature: 30
uniprot:Q920F5 [malonyl-CoA decarboxylase(Enzyme) wildtype]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 30 mM phosphate, 100 mM KCl, 75 mM sucrose
PH: 7
Temperature: 30
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) wildtype MCD]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant E302G]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant C243S]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase, 0.1 M H2O2
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant C243S]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase, 0.2 M H2O2
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant C243S]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) wildtype MCD]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase, 0.05 M H2O2
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant C206S]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase, 0.1 M H2O2
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant C206S/C243S]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant C206S/C243S]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase, 0.2 M H2O2
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant C206S]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase, 0.2 M H2O2
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) wildtype MCD]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase, 0.2 M H2O2
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) wildtype MCD]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase, 0.1 M H2O2
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant C206S/C243S]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase, 0.1 M H2O2
PH: 8.5
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant C206S]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris, 4 mM Malate, 4 mM NAD+, 89 U/ml malate dehydrogenase, 31 U/ml citrate synthase
PH: 8.5
Temperature: 36
uniprot:Q920F5 [malonyl-CoA decarboxylase(Enzyme) wildtype]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM sodium phosphate, 0.2 mM dithiothreitol, 4 mM malate, 0.2 mM NAD+, 20 U/ml malate dehydrogenase, 4 U/ml citrate synthase
PH: 7
Temperature: 36
uniprot:Q920F5 [malonyl-CoA decarboxylase(Enzyme) wildtype]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.1 mM DTE
PH: 8
Temperature: 30
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant delta39MCD]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 10 mM PBS, 25 mM K2HPO4/KH2PO4, 0.05 % Tween 20, 2 mM malate, 2 mM NAD+, 15.72 U/ml malic dehydrogenase, 0.56 U/ml citrate synthase
PH: 7.4
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) mutant delta39MCD]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 10 mM PBS, 25 mM K2HPO4/KH2PO4, 0.05 % Tween 20, 2 mM malate, 2 mM NAD+, 15.72 U/ml malic dehydrogenase, 0.56 U/ml citrate synthase
PH: 7.4
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) wildtype]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 10 mM PBS, 25 mM K2HPO4/KH2PO4, 0.05 % Tween 20, 2 mM malate, 2 mM NAD+, 15.72 U/ml malic dehydrogenase, 0.56 U/ml citrate synthase
PH: 7.4
Temperature: 36
uniprot:O95822 [malonyl-CoA decarboxylase(Enzyme) wildtype]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 10 mM PBS, 25 mM K2HPO4/KH2PO4, 0.05 % Tween 20, 2 mM malate, 2 mM NAD+, 15.72 U/ml malic dehydrogenase, 0.56 U/ml citrate synthase
PH: 7.4
Temperature: 36
uniprot:Q920F5 [Phosphate]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: potassium phosphate
PH: 7
Temperature: 25
uniprot:Q920F5 [Phosphate]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: potassium phosphate
PH: 7
Temperature: 25
uniprot:Q920F5 [Phosphate]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: potassium phosphate
PH: 7
Temperature: 25
- [Phosphate]

Malonyl_CoA -> Acetyl_CoA + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: potassium phosphate
PH: 7
Temperature: 25
- [Phosphate]

Malonyl_CoA -> CO2 + Acetyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: potassium phosphate
PH: 7
Temperature: 25

Pathways

pathway id name
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-RNO-390918 Peroxisomal lipid metabolism
Reactome:R-MMU-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-MMU-390918 Peroxisomal lipid metabolism
Reactome:R-RNO-390247 Beta-oxidation of very long chain fatty acids
WikiPathways:WP4686 Leucine, isoleucine and valine metabolism
PathBank:SMP0000016 Propanoate Metabolism
PathBank:SMP0000201 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0000198 Malonic Aciduria
PathBank:SMP0000502 Malonyl-CoA Decarboxylase Deficiency