EC: 2.5.1.61

hydroxymethylbilane synthase (porphobilinogen:(4-[2-carboxyethyl]-3-[carboxymethyl]pyrrol-2-yl)methyltransferase (hydrolysing))

enzyme lambda metabolic reaction experiment
uniprot:P08397 [hydroxymethylbilane synthase(Enzyme) mutant V215E]

H2O + 4 Porphobilinogen -> 4 NH3 + Hydroxymethylbilane		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 0.1 M DTT
PH: 8.2
Temperature: 37
uniprot:P08397 [hydroxymethylbilane synthase(Enzyme) mutant R167W]

4 Porphobilinogen + H2O -> 4 NH3 + Hydroxymethylbilane		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 0.1 M DTT
PH: 8.2
Temperature: 37
uniprot:P08397 [hydroxymethylbilane synthase(Enzyme) wildtype]

H2O + 4 Porphobilinogen -> 4 NH3 + Hydroxymethylbilane		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 0.1 M DTT
PH: 8.2
Temperature: 37
uniprot:P08397 [hydroxymethylbilane synthase(Enzyme) mutant K132N]

H2O + 4 Porphobilinogen -> 4 NH3 + Hydroxymethylbilane		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 0.1 M DTT
PH: 8.2
Temperature: 37
uniprot:P19356 [hydroxymethylbilane synthase(Enzyme) wildtype]

4 Porphobilinogen + H2O -> Hydroxymethylbilane + 4 NH3		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 60 mM NaCl, 12 mM MgCl2
PH: 8.2
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-5920 superpathway of b heme biosynthesis from glycine
BioCyc:HPY_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:HPY_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:CAULO_PWY-5918 superpathay of heme biosynthesis from glutamate
BioCyc:CAULO_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:AGRO_PWY-5918 superpathay of heme b biosynthesis from glutamate
BioCyc:CORYNE_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:GCF_000013425_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:HUMAN_PWY-5920 heme biosynthesis
BioCyc:YEAST_PWY-5189 tetrapyrrole biosynthesis II
BioCyc:MTBH37RV_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:CAULO_PWY-5920 superpathway of heme biosynthesis from glycine
BioCyc:MOUSE_PWY-5189 tetrapyrrole biosynthesis II
BioCyc:SYNEL_P381-PWY adenosylcobalamin biosynthesis II (late cobalt incorporation)
BioCyc:ECOL316407_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:CAULONA1000_PWY-5189 tetrapyrrole biosynthesis II
BioCyc:HUMAN_PWY-5189 tetrapyrrole biosynthesis
BioCyc:META_PWY-5189 tetrapyrrole biosynthesis II (from glycine)
BioCyc:ECO_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:YEAST_PWY3O-50 tetrapyrrole biosynthesis
BioCyc:YEAST_PWY-5920 superpathway of heme biosynthesis
BioCyc:ARA_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:ARA_PWYQT-62 superpathway of proto- and siroheme biosynthesis
BioCyc:CAULO_PWY-5189 tetrapyrrole biosynthesis II (from glycine)
BioCyc:BSUB_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:SYNEL_PWY-5918 superpathay of heme biosynthesis from glutamate
BioCyc:SYNEL_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:CAULONA1000_PWY-5188 tetrapyrrole biosynthesis I
BioCyc:META_PWY-5918 superpathay of heme b biosynthesis from glutamate
BioCyc:META_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:AGRO_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:MOUSE_PWY-5920 heme biosynthesis II
PathBank:SMP0000953 Porphyrin Metabolism
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-4827054 Tetrapyrrole biosynthesis I
Plant Reactome:R-OSA-2867929 Cofactor biosyntheses
PlantCyc:PLANT_PWY-5918 superpathay of heme b biosynthesis from glutamate
PlantCyc:ARA_PWYQT-62 superpathway of proto- and siroheme biosynthesis
PlantCyc:PLANT_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
PlantCyc:ARA_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
PlantCyc:ARA_PWY-5918 superpathay of heme b biosynthesis from glutamate
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-189445 Porphyrin metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-RNO-189445 Porphyrin metabolism
Reactome:R-SCE-189445 Porphyrin metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-BTA-189451 Heme biosynthesis
Reactome:R-HSA-189445 Porphyrin metabolism
Reactome:R-MMU-189445 Porphyrin metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-189451 Heme biosynthesis
Reactome:R-SCE-189451 Heme biosynthesis
Reactome:R-SPO-189445 Porphyrin metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-189451 Heme biosynthesis
Reactome:R-MMU-189451 Heme biosynthesis
Reactome:R-SPO-189451 Heme biosynthesis
PathBank:SMP0120492 Hereditary Coproporphyria (HCP)
PathBank:SMP0120544 Porphyria Variegata (PV)
PathBank:SMP0000024 Porphyrin Metabolism
PathBank:SMP0120447 Acute Intermittent Porphyria
PathBank:SMP0000345 Congenital Erythropoietic Porphyria (CEP) or Gunther Disease
PathBank:SMP0063655 Porphyrin Metabolism
PathBank:SMP0120469 Congenital Erythropoietic Porphyria (CEP) or Gunther Disease
PathBank:SMP0121321 Porphyrin Metabolism
PathBank:SMP0000344 Acute Intermittent Porphyria
PathBank:SMP0000346 Porphyria Variegata (PV)
PathBank:SMP0000342 Hereditary Coproporphyria (HCP)
PathBank:SMP0087243 Porphyrin Metabolism