EC: 1.1.1.45

L-gulonate 3-dehydrogenase (L-gulonate:NAD+ 3-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q9Y2S2 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NAD+ + L_Gulonate -> NADH + H+ + 3_Dehydro_L_gulonate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NAD+ + L_Gulonate -> H+ + 3_Dehydro_L_gulonate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NAD+ + L_Gulonate -> H+ + NADH + 3_Dehydro_L_gulonate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

Threonate + NAD+ -> H+ + 3_Dehydro_L_threonate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

Threonate + NAD+ -> H+ + NADH + 3_Dehydro_L_threonate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:Q9Y2S2 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NAD+ + (S)_3_Hydroxybutanoate -> Acetoacetate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NAD+ + (S)_3_Hydroxybutanoate -> NADH + H+ + Acetoacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

(S)_3_Hydroxybutanoate + NAD+ -> NADH + Acetoacetate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:Q9Y2S2 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NAD+ + L_Gulonate -> NADH + H+ + 3_Dehydro_L_gulonate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NAD+ + L_Gulonate -> 3_Dehydro_L_gulonate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

L_Gulonate + NAD+ -> 3_Dehydro_L_gulonate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:Q9Y2S2 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

Threonate + NAD+ -> NADH + 3_Dehydro_L_threonate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

Acetoacetate + NADH + H+ -> NAD+ + (S)_3_Hydroxybutanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

Acetoacetate + H+ + NADH -> (S)_3_Hydroxybutanoate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:Q9Y2S2 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

L_Gulonate + NADP+ -> H+ + 3_Dehydro_L_gulonate + NADPH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NADP+ + L_Gulonate -> H+ + NADPH + 3_Dehydro_L_gulonate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NADP+ + L_Gulonate -> 3_Dehydro_L_gulonate + H+ + NADPH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:Q9Y2S2 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NADH + H+ + Acetoacetate -> (S)_3_Hydroxybutanoate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:Q9Y2S2 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

H+ + NADH + Acetoacetate -> (S)_3_Hydroxybutanoate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NADH + Acetoacetate + H+ -> NAD+ + (S)_3_Hydroxybutanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NADH + H+ + Acetoacetate -> NAD+ + (S)_3_Hydroxybutanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NADH + 2,3_Diketogulonate + H+ -> NAD+ + 2_Ketogulonate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:Q9Y2S2 [Phosphate]

L_Gulonate + NAD+ -> 3_Dehydro_L_gulonate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) mutant D36R]

L_Gulonate + NAD+ -> 3_Dehydro_L_gulonate + H+ + NADH		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

(S)_3_Hydroxybutanoate + NAD+ -> NADH + H+ + Acetoacetate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype]

NADH + Acetoacetate + H+ -> NAD+ + (S)_3_Hydroxybutanoate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype + mutant S124A]

L_Gulonate + NAD+ -> NADH + H+ + 3_Dehydro_L_gulonate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) mutant S124A]

L_Gulonate + NAD+ -> H+ + 3_Dehydro_L_gulonate + NADH		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) mutant E157Q]

NAD+ + L_Gulonate -> 3_Dehydro_L_gulonate + NADH + H+		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype + mutant D36R]

L_Gulonate + NAD+ -> NADH + H+ + 3_Dehydro_L_gulonate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype + mutant E157Q]

L_Gulonate + NAD+ -> H+ + NADH + 3_Dehydro_L_gulonate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) mutant D36R]

NADP+ + L_Gulonate -> NADPH + H+ + 3_Dehydro_L_gulonate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) mutant E157Q]

L_Gulonate + NADP+ -> 3_Dehydro_L_gulonate + NADPH + H+		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype + mutant D36R]

L_Gulonate + NADP+ -> 3_Dehydro_L_gulonate + NADPH + H+		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) wildtype + mutant E157Q]

NADP+ + L_Gulonate -> NADPH + 3_Dehydro_L_gulonate + H+		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [Phosphate]

L_Gulonate + NAD+ -> H+ + 3_Dehydro_L_gulonate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [Phosphate]

NAD+ + L_Gulonate -> NADH + 3_Dehydro_L_gulonate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) mutant E157Q]

Acetoacetate + H+ + NADH -> NAD+ + (S)_3_Hydroxybutanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MOPS
PH: 7
Temperature: 25
uniprot:P14755 [L-gulonate 3-dehydrogenase(Enzyme) mutant S124A]

NADP+ + L_Gulonate -> H+ + NADPH + 3_Dehydro_L_gulonate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (KmA * B)) ) + (KmB * A)) ) + (KdA * KmB))) buffer: 50 mM MOPS
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-5525 D-glucuronate degradation
BioCyc:MOUSE_PWY-5525 D-glucuronate degradation I
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-5661270 Formation of xylulose-5-phosphate
Reactome:R-HSA-5661270 Formation of xylulose-5-phosphate
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-5661270 Formation of xylulose-5-phosphate
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-BTA-71387 Carbohydrate metabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-5661270 Formation of xylulose-5-phosphate
Reactome:R-HSA-1430728 Metabolism
Reactome:R-RNO-71387 Carbohydrate metabolism