EC: 2.4.2.36

NAD+-diphthamide ADP-ribosyltransferase (NAD+:diphthamide-[translation elongation factor 2] N-(ADP-D-ribosyl)transferase)

enzyme lambda metabolic reaction experiment
uniprot:P11439 [NAD+---diphthamide ADP-ribosyltransferase(Enzyme) wildtype fragment PE24H]

Peptide_diphthamide + NAD+ -> Nicotinamide + Peptide_N_(ADP_D_ribosyl)diphthamide		 (( (( (E * kcat) ) * (S ^ n)) ) / (Km + (S ^ n))) buffer: 20 mM Tris-HCl, 100 mM KCl
PH: 7.9
Temperature: 25
uniprot:P11439 [NAD+---diphthamide ADP-ribosyltransferase(Enzyme) wildtype fragment PE24H]

NAD+ + Peptide_diphthamide -> Peptide_N_(ADP_D_ribosyl)diphthamide + Nicotinamide		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 50 mM NaCl
PH: 7.9
Temperature: 25
uniprot:P11439 [NAD+---diphthamide ADP-ribosyltransferase(Enzyme) wildtype C-terminal fragment PE24]

NAD+ + Peptide_diphthamide -> Peptide_N_(ADP_D_ribosyl)diphthamide + Nicotinamide		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Tris-HCl, 50 mM KCl
PH: 7.8
Temperature: 25
uniprot:P11439 [NAD+---diphthamide ADP-ribosyltransferase(Enzyme) wildtype C-terminal fragment PE24]

NAD+ + Peptide_diphthamide -> Peptide_N_(ADP_D_ribosyl)diphthamide + Nicotinamide		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-HCl, 50 mM KCl
PH: 7.8
Temperature: 25

Pathways

pathway id name
Reactome:R-HSA-9824439 Bacterial Infection Pathways
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-5339562 Uptake and actions of bacterial toxins
Reactome:R-HSA-5336415 Uptake and function of diphtheria toxin
Reactome:R-HSA-5663205 Infectious disease