flavin biosynthesis I (bacteria and plants)

Birkenmeier M, Neumann S, Röder T. Kinetic modeling of riboflavin biosynthesis in Bacillus subtilis under production conditions. Biotechnol Lett. 2014 May;36(5):919–28. doi: 10.1007/s10529-013-1435-8. PMID: 24442413.

Metabolites

5-Amino-6-(1-D-ribitylamino)uracil

Formula: C9H16N4O6 (276.10697960000005)

CAS ID: 17014-74-3

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Manganese(2+)

Formula: Mn (54.938046)

CAS ID: 7439-96-5

5-amino-6-(5-phospho-D-ribosylamino)uracil

Formula: C9H13N4O9P (352.0420138)

CAS ID:

formate

Formula: CHO2 (44.997654600000004)

CAS ID: 71-47-6

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.1.1.193 5-amino-6-(5-phosphoribosylamino)uracil reductase 5-amino-6-(5-phospho-D-ribitylamino)uracil:NADP+ 1'-oxidoreductase
2.5.1.78 6,7-dimethyl-8-ribityllumazine synthase 5-amino-6-(D-ribitylamino)uracil butanedionetransferase
2.5.1.9 riboflavin synthase 6,7-dimethyl-8-(1-D-ribityl)lumazine:6,7-dimethyl-8-(1-D-ribityl)lumazine 2,3-butanediyltransferase
2.7.1.26 riboflavin kinase ATP:riboflavin 5'-phosphotransferase
2.7.7.2 FAD synthase ATP:FMN adenylyltransferase
3.1.3.104 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphohydrolase
3.5.4.25 GTP cyclohydrolase II GTP 7,8-8,9-dihydrolase (formate-releasing, phosphate-releasing)
3.5.4.26 diaminohydroxyphosphoribosylaminopyrimidine deaminase 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine 2-aminohydrolase
4.1.99.12 3,4-dihydroxy-2-butanone-4-phosphate synthase D-ribulose 5-phosphate formate-lyase (L-3,4-dihydroxybutan-2-one 4-phosphate-forming)


Proteins

Protein ID name full name
P11998 ribH 6,7-dimethyl-8-ribityllumazine synthase
P16440 ribE Riboflavin synthase
P17618 ribD Riboflavin biosynthesis protein RibD
P17620 ribBA Riboflavin biosynthesis protein RibBA
P42962 ycsE 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YcsE
P54575 ribC Bifunctional riboflavin kinase/FMN adenylyltransferase