xanthine and xanthosine salvage

Dandanell G, Szczepanowski RH, Kierdaszuk B, Shugar D, Bochtler M. Escherichia coli Purine Nucleoside Phosphorylase II, the Product of the xapA Gene. Journal of Molecular Biology. 2005 Apr;348(1):113–25. doi: 10.1016/j.jmb.2005.02.019.; Guddat LW, Vos S, Martin JL, Keough DT, de Jersey J. Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase. Protein Science. 2002 Jul;11(7):1626–38. doi: 10.1110/ps.0201002.; Maynes JT, Yuan RG, Snyder FF. Identification, Expression, and Characterization of Escherichia coli Guanine Deaminase. J Bacteriol. 2000 Aug 15;182(16):4658–60. doi: 10.1128/jb.182.16.4658-4660.2000.; Vos S, de Jersey J, Martin JL. Crystal structure of Escherichia coli xanthine phosphoribosyltransferase. Biochemistry. 1997 Apr 08;36(14):4125–34. doi: 10.1021/bi962640d. PMID: 9100006.; Koszalka GW, Vanhooke J, Short SA, Hall WW. Purification and properties of inosine-guanosine phosphorylase from Escherichia coli K-12. J Bacteriol. 1988 Aug;170(8):3493–8. doi: 10.1128/jb.170.8.3493-3498.1988.; Bzowska A, Kulikowska E, Darzynkiewicz E, Shugar D. Purine nucleoside phosphorylase. Structure-activity relationships for substrate and inhibitor properties of N-1-, N-7-, and C-8-substituted analogues; differentiation of mammalian and bacterial enzymes with N-1-methylinosine and guanosine. Journal of Biological Chemistry. 1988 Jul;263(19):9212–7. doi: 10.1016/s0021-9258(19)76527-2.; Deo SS, Wen Chen Tseng, Saini R, Coles RS, Athwal RS. Purification and characterization of Escherichia coli xanthine-guanine phosphoribosyltransferase produced by plasmid pSV2gpt. Biochimica et Biophysica Acta (BBA) - General Subjects. 1985 May;839(3):233–9. doi: 10.1016/0304-4165(85)90003-0.; Liu SW, Milman G. Purification and characterization of Escherichia coli guanine-xanthine phosphoribosyltransferase produced by a high efficiency expression plasmid utilizing a lambda PL promoter and CI857 temperature-sensitive repressor. Journal of Biological Chemistry. 1983 Jun;258(12):7469–75. doi: 10.1016/s0021-9258(18)32201-4.; Hochstadt J. Hypoxanthine phosphoribosyltransferase and guanine phosphoribosyltransferase from enteric bacteria. Methods Enzymol. 1978;51():549–58. doi: 10.1016/s0076-6879(78)51077-x. PMID: 692401.; Holden JA, Harriman PD, Wall JD. Escherichia coli mutants deficient in guanine-xanthine phosphoribosyltransferase. J Bacteriol. 1976 Jun;126(3):1141–8. doi: 10.1128/jb.126.3.1141-1148.1976.; Jensen KF, Nygaard P. Purine nucleoside phosphorylase from Escherichia coli and Salmonella typhimurium. Purification and some properties. Eur J Biochem. 1975 Feb 03;51(1):253–65. doi: 10.1111/j.1432-1033.1975.tb03925.x. PMID: 235429.

Metabolites

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Xanthine

Formula: C5H4N4O2 (152.0334244)

CAS ID: 69-89-6

Xanthosine

Formula: C10H12N4O6 (284.07568119999996)

CAS ID: 146-80-5

8-Azaguanine

Formula: C4H4N6O (152.0446574)

CAS ID: 134-58-7



Enzyme

EC Number name full name note
2.4.2.-
2.4.2.1 purine-nucleoside phosphorylase purine-nucleoside:phosphate ribosyltransferase
2.4.2.2 pyrimidine-nucleoside phosphorylase pyrimidine-nucleoside:phosphate (2'-deoxy)-alpha-D-ribosyltransferase
2.4.2.22 xanthine phosphoribosyltransferase XMP:diphosphate 5-phospho-alpha-D-ribosyltransferase


Proteins

Protein ID name full name
P0A9M5 gpt Xanthine-guanine phosphoribosyltransferase
P0C037 ppnP Pyrimidine/purine nucleoside phosphorylase
P45563 xapA Purine nucleoside phosphorylase 2