2-oxoglutarate decarboxylation to succinyl-CoA

Shim DJ, Nemeria NS, Balakrishnan A, Patel H, Song J, Wang J, Jordan F, Farinas ET. Assignment of Function to Histidines 260 and 298 by Engineering the E1 Component of the Escherichia coli 2-Oxoglutarate Dehydrogenase Complex; Substitutions That Lead to Acceptance of Substrates Lacking the 5-Carboxyl Group. Biochemistry. 2011 Aug 10;50(35):7705–9. doi: 10.1021/bi200936n.; Jones D , Perham R. The role of loop and ß-turn residues as structural and functional determinants for the lipoyl domain from the Escherichia coli 2-oxoglutarate dehydrogenase complex. 2007 Dec 21;409(2):357–66. doi: 10.1042/bj20071119.; Guest JR, Creaghan IT. Gene-protein relationships of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and characterization of lipoamide dehydrogenase mutants. J Gen Microbiol. 1973 Mar;75(1):197–210. doi: 10.1099/00221287-75-1-197. PMID: 4578971.

Metabolites

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

oxaloacetate(2-)

Formula: C4H2O5 (129.9902242)

CAS ID: 149-63-3

2-oxoglutarate(2-)

Formula: C5H4O5 (144.00587339999998)

CAS ID: 64-15-3

thiamine(1+) diphosphate(3-)

Formula: C12H16N4O7P2S (422.0214926)

CAS ID:

NADH(2-)

Formula: C21H27N7O14P2 (663.1091182000001)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9



Enzyme

EC Number name full name note
1.2.4.2 oxoglutarate dehydrogenase (succinyl-transferring) 2-oxoglutarate:[dihydrolipoyllysine-residue succinyltransferase]-lipoyllysine 2-oxidoreductase (decarboxylating, acceptor-succinylating)
1.8.1.4 dihydrolipoyl dehydrogenase protein-N6-(dihydrolipoyl)lysine:NAD+ oxidoreductase
2.3.1.61 dihydrolipoyllysine-residue succinyltransferase succinyl-CoA:enzyme-N6-(dihydrolipoyl)lysine S-succinyltransferase


Proteins

Protein ID name full name
P0A9P0 lpdA Dihydrolipoyl dehydrogenase
P0AFG3 sucA 2-oxoglutarate dehydrogenase E1 component
P0AFG6 sucB Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex