pyruvate decarboxylation to acetyl CoA

Fries M, Jung HI, Perham RN. Reaction mechanism of the heterotetrameric (alpha2beta2) E1 component of 2-oxo acid dehydrogenase multienzyme complexes. Biochemistry. 2003 Jun 17;42(23):6996–7002. doi: 10.1021/bi027397z. PMID: 12795594.; Milne JL, Shi D, Rosenthal PB, Sunshine JS, Domingo GJ, Wu X, Brooks BR, Perham RN, Henderson R, Subramaniam S. Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: a multifunctional catalytic machine. EMBO J. 2002 Nov 01;21(21):5587–98. PMID: 12411477; PMCID: PMC131071.; de Kok A, Hengeveld AF, Martin A, Westphal AH. The pyruvate dehydrogenase multi-enzyme complex from Gram-negative bacteria. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1998 Jun;1385(2):353–66. doi: 10.1016/s0167-4838(98)00079-x.

Metabolites

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

pyruvate

Formula: C3H3O3 (87.00821880000001)

CAS ID: 57-60-3

NADH(2-)

Formula: C21H27N7O14P2 (663.1091182000001)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9



Enzyme

EC Number name full name note
1.2.4.1 pyruvate dehydrogenase (acetyl-transferring) pyruvate:[dihydrolipoyllysine-residue acetyltransferase]-lipoyllysine 2-oxidoreductase (decarboxylating, acceptor-acetylating)
1.8.1.4 dihydrolipoyl dehydrogenase protein-N6-(dihydrolipoyl)lysine:NAD+ oxidoreductase
2.3.1.12 dihydrolipoyllysine-residue acetyltransferase acetyl-CoA:enzyme N6-(dihydrolipoyl)lysine S-acetyltransferase


Proteins

Protein ID name full name
P06959 aceF Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
P0A9P0 lpdA Dihydrolipoyl dehydrogenase
P0AFG8 aceE Pyruvate dehydrogenase E1 component