glutathione-mediated detoxification II

Wünschmann J, Krajewski M, Letzel T, Huber EM, Ehrmann A, Grill E, Lendzian KJ. Dissection of glutathione conjugate turnover in yeast. Phytochemistry. 2010 Jan;71(1):54–61. doi: 10.1016/j.phytochem.2009.09.034. PMID: 19897216.; Brazier-Hicks M, Evans KM, Cunningham OD, Hodgson DR, Steel PG, Edwards R. Catabolism of glutathione conjugates in Arabidopsis thaliana. Role in metabolic reactivation of the herbicide safener fenclorim. J Biol Chem. 2008 Jul 25;283(30):21102–12. PMID: 18522943; PMCID: PMC3258958.; Grzam A, Tennstedt P, Clemens S, Hell R, Meyer AJ. Vacuolar sequestration of glutathione S-conjugates outcompetes a possible degradation of the glutathione moiety by phytochelatin synthase. FEBS Lett. 2006 Nov 27;580(27):6384–90. doi: 10.1016/j.febslet.2006.10.050. PMID: 17097087.; Ishikawa T. The ATP-dependent glutathione S-conjugate export pump. Trends Biochem Sci. 1992 Nov;17(11):463–8. doi: 10.1016/0968-0004(92)90489-v. PMID: 1455517.

Metabolites

Cadmium

Formula: Cd (113.903361)

CAS ID: 7440-43-9

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

pyruvate

Formula: C3H3O3 (87.00821880000001)

CAS ID: 57-60-3

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

pyridoxal 5'-phosphate(2-)

Formula: C8H8NO6P (245.0089238)

CAS ID:



Enzyme

EC Number name full name note
2.3.2.15 glutathione gamma-glutamylcysteinyltransferase glutathione:poly(4-glutamyl-cysteinyl)glycine 4-glutamylcysteinyltransferase
2.3.2.2 gamma-glutamyltransferase (5-L-glutamyl)-peptide:amino-acid 5-glutamyltransferase
2.5.1.18 glutathione transferase RX:glutathione R-transferase
3.4.16.5 carboxypeptidase C
3.4.19.13 glutathione gamma-glutamate hydrolase
4.4.1.13 cysteine-S-conjugate beta-lyase L-cysteine-S-conjugate thiol-lyase (deaminating; pyruvate-forming)
7.2.2.2 ABC-type Cd2+ transporter ATP phosphohydrolase (ABC-type, heavy-metal-exporting)
7.6.2.3 ABC-type glutathione-S-conjugate transporter ATP + H2O + glutathione-S-conjugate[side 1] = ADP + phosphate + glutathione-S-conjugate[side 2]


Proteins

Protein ID name full name
P00729 PRC1 Carboxypeptidase Y
P38109 ATG42 Vacuolar serine-type carboxypeptidase ATG42
P39109 YCF1 Metal resistance protein YCF1
P40582 GTT1 Glutathione S-transferase 1
P43623 IRC7 Putative cystathionine beta-lyase
P53101 STR3 Cystathionine beta-lyase
Q05902 ECM38 Glutathione hydrolase proenzyme
Q12390 GTT2 Glutathione S-transferase 2