bisabolene biosynthesis (engineered)

Bohlmann J, Crock J, Jetter R, Croteau R. Terpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible ( E )-a-bisabolene synthase from grand fir ( Abies grandis ). Proc. Natl. Acad. Sci. U.S.A. 1998 Jun 09;95(12):6756–61. doi: 10.1073/pnas.95.12.6756.

Metabolites

(E)-gamma-Bisabolene

Formula: C15H24 (204.18779039999998)

CAS ID: 13062-00-5

(S)-beta-Bisabolene

Formula: C15H24 (204.18779039999998)

CAS ID: 495-61-4

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Zinc cation

Formula: Zn (63.929145)

CAS ID: 23713-49-7

iodoacetamide

Formula: C2H4INO (184.9337644)

CAS ID: 144-48-9



Enzyme

EC Number name full name note
2.5.1.10 (2E,6E)-farnesyl diphosphate synthase geranyl-diphosphate:isopentenyl-diphosphate geranyltranstransferase
4.2.3.56 gamma-humulene synthase (2E,6E)-farnesyl-diphosphate diphosphate-lyase (gamma-humulene-forming)
4.2.3.71 (E,E)-germacrene B synthase (2E,6E)-farnesyl-diphosphate diphosphate-lyase [(E,E)-germacrene-B-forming]
4.2.3.76 (+)-delta-selinene synthase (2E,6E)-farnesyl-diphosphate diphosphate-lyase [(+)-delta-selinene-forming]
5.3.3.2 isopentenyl-diphosphate DELTA-isomerase 3-methylbut-3-en-1-yl-diphosphate DELTA3-DELTA2-isomerase


Proteins

Protein ID name full name
P22939 ispA Farnesyl diphosphate synthase
Q46822 idi Isopentenyl-diphosphate Delta-isomerase