Synthesis of diphthamide-EEF2
Schaffrath R, Abdel-Fattah W, Klassen R, Stark MJR. The diphthamide modification pathway from Saccharomyces cerevisiae – revisited. Molecular Microbiology. 2014 Nov 17;94(6):1213–26. doi: 10.1111/mmi.12845.; Lin Z, Su X, Chen W, Ci B, Zhang S, Lin H. Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesis. J Am Chem Soc. 2014 Apr 30;136(17):6179–82. PMID: 24739148; PMCID: PMC4015618.; Su X, Lin Z, Lin H. The biosynthesis and biological function of diphthamide. Critical Reviews in Biochemistry and Molecular Biology. 2013 Aug 23;48(6):515–21. doi: 10.3109/10409238.2013.831023.; Uthman S, Bär C, Scheidt V, Liu S, ten Have S, Giorgini F, Stark MJR, Schaffrath R. The Amidation Step of Diphthamide Biosynthesis in Yeast Requires DPH6, a Gene Identified through Mining the DPH1-DPH5 Interaction Network. PLoS Genet. 2013 Feb 28;9(2):e1003334. doi: 10.1371/journal.pgen.1003334.; Liu S, Milne GT, Kuremsky JG, Fink GR, Leppla SH. Identification of the Proteins Required for Biosynthesis of Diphthamide, the Target of Bacterial ADP-Ribosylating Toxins on Translation Elongation Factor 2. Molecular and Cellular Biology. 2004 Nov 01;24(21):9487–97. doi: 10.1128/mcb.24.21.9487-9497.2004.; Van Ness BG, Howard JB, Bodley JW. Isolation and properties of the trypsin-derived ADP-ribosyl peptide from diphtheria toxin-modified yeast elongation factor 2. Journal of Biological Chemistry. 1978 Dec;253(24):8687–90. doi: 10.1016/s0021-9258(17)34230-8.; Pappenheimer AM. Diphtheria toxin. Annu Rev Biochem. 1977;46():69–94. doi: 10.1146/annurev.bi.46.070177.000441. PMID: 20040.; Collier RJ. Diphtheria toxin: mode of action and structure. Bacteriol Rev. 1975 Mar;39(1):54–85. doi: 10.1128/br.39.1.54-85.1975.