Synthesis of PIPs at the late endosome membrane

Sbrissa D, Ikonomov OC, Fenner H, Shisheva A. ArPIKfyve Homomeric and Heteromeric Interactions Scaffold PIKfyve and Sac3 in a Complex to Promote PIKfyve Activity and Functionality. Journal of Molecular Biology. 2008 Dec;384(4):766–79. doi: 10.1016/j.jmb.2008.10.009.; Cao C, Backer JM, Laporte J, Bedrick EJ, Wandinger-Ness A. Sequential actions of myotubularin lipid phosphatases regulate endosomal PI(3)P and growth factor receptor trafficking. Mol Biol Cell. 2008 Aug;19(8):3334–46. PMID: 18524850; PMCID: PMC2488308.; Sbrissa D, Ikonomov OC, Fu Z, Ijuin T, Gruenberg J, Takenawa T, Shisheva A. Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex. J Biol Chem. 2007 Aug 17;282(33):23878–91. doi: 10.1074/jbc.m611678200. PMID: 17556371.; Cao C, Laporte J, Backer JM, Wandinger-Ness A, Stein MP. Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes. Traffic. 2007 Aug;8(8):1052–67. doi: 10.1111/j.1600-0854.2007.00586.x. PMID: 17651088.; Kim S, Taylor GS, Torgersen KM, Dixon JE. Myotubularin and MTMR2, Phosphatidylinositol 3-Phosphatases Mutated in Myotubular Myopathy and Type 4B Charcot-Marie-Tooth Disease. Journal of Biological Chemistry. 2002 Feb;277(6):4526–31. doi: 10.1074/jbc.m111087200.; Arcaro A, Zvelebil MJ, Wallasch C, Ullrich A, Waterfield MD, Domin J. Class II Phosphoinositide 3-Kinases Are Downstream Targets of Activated Polypeptide Growth Factor Receptors. Molecular and Cellular Biology. 2000 Jun 01;20(11):3817–30. doi: 10.1128/mcb.20.11.3817-3830.2000.

Metabolites

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Manganese(2+)

Formula: Mn (54.938046)

CAS ID: 7439-96-5

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:



Enzyme

EC Number name full name note
2.7.1.137 phosphatidylinositol 3-kinase ATP:1-phosphatidyl-1D-myo-inositol 3-phosphotransferase
2.7.11.1 non-specific serine/threonine protein kinase ATP:protein phosphotransferase (non-specific)


Proteins

Protein ID name full name
O42900 vps15 Serine/threonine-protein kinase vps15
P50520 pik3 Phosphatidylinositol 3-kinase pik3