Zymostenol biosynthesis via lathosterol (Kandutsch-Russell pathway)

Mitsche MA, McDonald JG, Hobbs HH, Cohen JC. Flux analysis of cholesterol biosynthesis in vivo reveals multiple tissue and cell-type specific pathways. Elife. 2015 Jun 26;4():e07999. PMID: 26114596; PMCID: PMC4501332.

Metabolites

24,25-Dihydrolanosterol

Formula: C30H52O (428.4017942)

CAS ID: 79-62-9

4,4-Dimethyl-5alpha-cholesta-8-en-3beta-ol

Formula: C29H50O (414.386145)

CAS ID: 7240-15-5

4alpha-Methylcholesta-8-en-3beta-ol

Formula: C28H48O (400.37049579999996)

CAS ID: 5241-22-5

5alpha-Cholest-8-en-3beta-ol

Formula: C27H46O (386.3548466)

CAS ID: 566-97-2

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

Lanosterol

Formula: C30H50O (426.386145)

CAS ID: 79-63-0

4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol

Formula: C29H48O (412.37049579999996)

CAS ID: 19456-83-8

4α-methyl-5α-cholesta-8-en-3-one

Formula: C28H46O (398.3548466)

CAS ID:

5alpha-cholest-8-en-3-one

Formula: C27H44O (384.3391974)

CAS ID:

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.1.1.170 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating) 3beta-hydroxysteroid-4alpha-carboxylate:NAD(P)+ 3-oxidoreductase (decarboxylating)
1.1.1.210 3beta(or 20alpha)-hydroxysteroid dehydrogenase 3beta(or 20alpha)-hydroxysteroid:NADP+ oxidoreductase
1.1.1.270 3beta-hydroxysteroid 3-dehydrogenase 3beta-hydroxysteroid:NADP+ 3-oxidoreductase
1.1.1.62 17beta-estradiol 17-dehydrogenase 17beta-estradiol:NAD(P)+ 17-oxidoreductase
1.3.1.72 DELTA24-sterol reductase sterol:NADP+ DELTA24-oxidoreductase


Proteins

Protein ID name full name