Homologous DNA Pairing and Strand Exchange

Park JY, Singh TR, Nassar N, Zhang F, Freund M, Hanenberg H, Meetei AR, Andreassen PR. Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair. Oncogene. 2014 Oct 02;33(40):4803–12. PMID: 24141787; PMCID: PMC3994186.; Chun J, Buechelmaier ES, Powell SN. Rad51 Paralog Complexes BCDX2 and CX3 Act at Different Stages in the BRCA1-BRCA2-Dependent Homologous Recombination Pathway. Molecular and Cellular Biology. 2013 Jan 01;33(2):387–95. doi: 10.1128/mcb.00465-12.; Ciccia A, Elledge SJ. The DNA Damage Response: Making It Safe to Play with Knives. Molecular Cell. 2010 Oct;40(2):179–204. doi: 10.1016/j.molcel.2010.09.019.; Buisson R, Dion-Côté A, Coulombe Y, Launay H, Cai H, Stasiak AZ, Stasiak A, Xia B, Masson J. Cooperation of breast cancer proteins PALB2 and piccolo BRCA2 in stimulating homologous recombination. Nature Structural & Molecular Biology. 2010 Sep 26;17(10):1247–54. doi: 10.1038/nsmb.1915.; Dray E, Etchin J, Wiese C, Saro D, Williams GJ, Hammel M, Yu X, Galkin VE, Liu D, Tsai M, Sy SM, Schild D, Egelman E, Chen J, Sung P. Enhancement of RAD51 recombinase activity by the tumor suppressor PALB2. Nature Structural & Molecular Biology. 2010 Sep 26;17(10):1255–9. doi: 10.1038/nsmb.1916.; Sy SMH, Huen MSY, Chen J. PALB2 is an integral component of the BRCA complex required for homologous recombination repair. Proc. Natl. Acad. Sci. U.S.A. 2009 Apr 28;106(17):7155–60. doi: 10.1073/pnas.0811159106.; Zhang F, Ma J, Wu J, Ye L, Cai H, Xia B, Yu X. PALB2 Links BRCA1 and BRCA2 in the DNA-Damage Response. Current Biology. 2009 Mar;19(6):524–9. doi: 10.1016/j.cub.2009.02.018.; Modesti M, Budzowska M, Baldeyron C, Demmers JA, Ghirlando R, Kanaar R. RAD51AP1 is a structure-specific DNA binding protein that stimulates joint molecule formation during RAD51-mediated homologous recombination. Mol Cell. 2007 Nov 09;28(3):468–81. doi: 10.1016/j.molcel.2007.08.025. PMID: 17996710.; Wiese C, Dray E, Groesser T, San Filippo J, Shi I, Collins DW, Tsai M, Williams GJ, Rydberg B, Sung P, Schild D. Promotion of Homologous Recombination and Genomic Stability by RAD51AP1 via RAD51 Recombinase Enhancement. Molecular Cell. 2007 Nov;28(3):482–90. doi: 10.1016/j.molcel.2007.08.027.; Sung P, Krejci L, Van Komen S, Sehorn MG. Rad51 Recombinase and Recombination Mediators. Journal of Biological Chemistry. 2003 Oct;278(44):42729–32. doi: 10.1074/jbc.r300027200.; Sigurdsson S, Van Komen S, Petukhova G, Sung P. Homologous DNA Pairing by Human Recombination Factors Rad51 and Rad54. Journal of Biological Chemistry. 2002 Nov;277(45):42790–4. doi: 10.1074/jbc.m208004200.; Gupta RC, Folta-Stogniew E, Radding CM. Human Rad51 Protein Can Form Homologous Joints in the Absence of Net Strand Exchange. Journal of Biological Chemistry. 1999 Jan;274(3):1248–56. doi: 10.1074/jbc.274.3.1248.

Metabolites

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:



Enzyme

EC Number name full name note
2.3.1.-
2.3.1.48 histone acetyltransferase acetyl-CoA:[protein]-L-lysine acetyltransferase
2.7.11.1 non-specific serine/threonine protein kinase ATP:protein phosphotransferase (non-specific)
5.6.2.3 DNA 5'-3' helicase DNA 5'-3' helicase (ATP-hydrolysing)


Proteins

Protein ID name full name