Cholesterol biosynthesis from zymosterol (modified Kandutsch-Russell pathway)

Mitsche MA, McDonald JG, Hobbs HH, Cohen JC. Flux analysis of cholesterol biosynthesis in vivo reveals multiple tissue and cell-type specific pathways. Elife. 2015 Jun 26;4():e07999. PMID: 26114596; PMCID: PMC4501332.

Metabolites

5alpha-Cholest-8-en-3beta-ol

Formula: C27H46O (386.3548466)

CAS ID: 566-97-2

7-Dehydrocholesterol

Formula: C27H44O (384.3391974)

CAS ID: 434-16-2

Cholesterol

Formula: C27H46O (386.3548466)

CAS ID: 57-88-5

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Lathosterol

Formula: C27H46O (386.3548466)

CAS ID: 80-99-9

Oxygen

Formula: O2 (31.98983)

CAS ID: 7782-44-7

Zymosterol

Formula: C27H44O (384.3391974)

CAS ID: 128-33-6

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.14.19.20 DELTA7-sterol 5(6)-desaturase DELTA7-sterol,ferrocytochrome b5:oxygen oxidoreductase 5,6-dehydrogenating
1.3.1.21 7-dehydrocholesterol reductase cholesterol:NADP+ DELTA7-oxidoreductase
1.3.1.72 DELTA24-sterol reductase sterol:NADP+ DELTA24-oxidoreductase
3.3.2.11 cholesterol-5,6-oxide hydrolase 5,6alpha-epoxy-5alpha-cholestan-3beta-ol hydrolase
5.3.3.5 cholestenol DELTA-isomerase DELTA7-Cholestenol DELTA7-DELTA8-isomerase


Proteins

Protein ID name full name