enterobacterial common antigen biosynthesis

Hung MN, Rangarajan E, Munger C, Nadeau G, Sulea T, Matte A. Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesis. J Bacteriol. 2006 Aug;188(15):5606–17. PMID: 16855251; PMCID: PMC1540030.; Danese PN, Oliver GR, Barr K, Bowman GD, Rick PD, Silhavy TJ. Accumulation of the Enterobacterial Common Antigen Lipid II Biosynthetic Intermediate Stimulates degP Transcription in Escherichia coli. J Bacteriol. 1998 Nov 15;180(22):5875–84. doi: 10.1128/jb.180.22.5875-5884.1998.

Metabolites

3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate

Formula: C32H58N2O7S (614.3964518)

CAS ID: 75621-03-3

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Potassium cation

Formula: K (38.963708)

CAS ID: 24203-36-9

dTDP-alpha-D-glucose

Formula: C16H24N2O16P2 (562.0601044)

CAS ID:

2-oxoglutarate(2-)

Formula: C5H4O5 (144.00587339999998)

CAS ID: 64-15-3

NADH(2-)

Formula: C21H27N7O14P2 (663.1091182000001)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0

dTDP(3-)

Formula: C10H13N2O11P2 (398.9994588)

CAS ID:

pyridoxal 5'-phosphate(2-)

Formula: C8H8NO6P (245.0089238)

CAS ID:



Enzyme

EC Number name full name note
1.1.1.336 UDP-N-acetyl-D-mannosamine dehydrogenase UDP-N-acetyl-alpha-D-mannosamine:NAD+ 6-oxidoreductase
2.3.1.210 dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase acetyl-CoA:dTDP-4-amino-4,6-dideoxy-alpha-D-galactose N-acetyltransferase
2.4.1.180 lipopolysaccharide N-acetylmannosaminouronosyltransferase UDP-N-acetyl-beta-D-mannosaminouronate:lipid I N-acetyl-beta-D-mannosaminouronosyltransferase
2.4.1.325 TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase TDP-N-acetyl-alpha-D-fucose:N-acetyl-beta-D-mannosaminouronyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol N-acetylfucosaminyltransferase
2.6.1.59 dTDP-4-amino-4,6-dideoxygalactose transaminase dTDP-4,6-dideoxy-D-galactose:2-oxoglutarate aminotransferase
2.7.7.24 glucose-1-phosphate thymidylyltransferase dTTP:alpha-D-glucose-1-phosphate thymidylyltransferase
2.7.8.33 UDP-N-acetylglucosamine-undecaprenyl-phosphate N-acetylglucosaminephosphotransferase UDP-N-acetyl-D-glucosamine:ditrans,octacis-undecaprenyl phosphate N-acetyl-D-glucosaminephosphotransferase
4.2.1.46 dTDP-glucose 4,6-dehydratase dTDP-glucose 4,6-hydro-lyase (dTDP-4-dehydro-6-deoxy-D-glucose-forming)
5.1.3.14 UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing) UDP-N-acetyl-alpha-D-glucosamine 2-epimerase


Proteins

Protein ID name full name
P0AC78 wecA Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase
P27828 wecB UDP-N-acetylglucosamine 2-epimerase
P27829 wecC UDP-N-acetyl-D-mannosamine dehydrogenase
P27830 rffG dTDP-glucose 4,6-dehydratase 2
P27832 wecD dTDP-fucosamine acetyltransferase
P27833 wecE dTDP-4-amino-4,6-dideoxygalactose transaminase
P27836 wecG UDP-N-acetyl-D-mannosaminuronic acid transferase
P37744 rfbA Glucose-1-phosphate thymidylyltransferase 1
P56258 wecF TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase
P61887 rffH Glucose-1-phosphate thymidylyltransferase 2