EC: 3.2.2.9

adenosylhomocysteine nucleosidase (S-adenosyl-L-homocysteine homocysteinylribohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant S196A]

5'_Deoxy_5'_(methylthio)adenosine + H2O -> Adenine + 5_Methylthio_D_ribose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant F151A]

H2O + 5'_Deoxy_5'_(methylthio)adenosine -> Adenine + 5_Methylthio_D_ribose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant M173A]

H2O + 5'_Deoxy_5'_(methylthio)adenosine -> 5_Methylthio_D_ribose + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) wildtype]

5'_Deoxy_5'_(methylthio)adenosine + H2O -> 5_Methylthio_D_ribose + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant R193A]

5'_Deoxy_5'_(methylthio)adenosine + H2O -> 5_Methylthio_D_ribose + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant S76A]

H2O + 5'_Deoxy_5'_(methylthio)adenosine -> 5_Methylthio_D_ribose + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant F207A]

5'_Deoxy_5'_(methylthio)adenosine + H2O -> Adenine + 5_Methylthio_D_ribose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant V102A]

5'_Deoxy_5'_(methylthio)adenosine + H2O -> Adenine + 5_Methylthio_D_ribose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant M9A]

H2O + 5'_Deoxy_5'_(methylthio)adenosine -> 5_Methylthio_D_ribose + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant I50A]

H2O + 5'_Deoxy_5'_(methylthio)adenosine -> Adenine + 5_Methylthio_D_ribose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant Y107F]

5'_Deoxy_5'_(methylthio)adenosine + H2O -> 5_Methylthio_D_ribose + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant F105A]

H2O + 5'_Deoxy_5'_(methylthio)adenosine -> 5_Methylthio_D_ribose + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) wildtype]

H2O + S_Adenosyl_L_homocysteine -> Adenine + S_Ribosyl_L_homocysteine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant S196A]

H2O + S_Adenosyl_L_homocysteine -> Adenine + S_Ribosyl_L_homocysteine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant F151A]

S_Adenosyl_L_homocysteine + H2O -> Adenine + S_Ribosyl_L_homocysteine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant V102A]

H2O + S_Adenosyl_L_homocysteine -> S_Ribosyl_L_homocysteine + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant R193A]

S_Adenosyl_L_homocysteine + H2O -> S_Ribosyl_L_homocysteine + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant M173A]

H2O + S_Adenosyl_L_homocysteine -> Adenine + S_Ribosyl_L_homocysteine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant M9A]

H2O + S_Adenosyl_L_homocysteine -> S_Ribosyl_L_homocysteine + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant I50A]

S_Adenosyl_L_homocysteine + H2O -> Adenine + S_Ribosyl_L_homocysteine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant F207A]

S_Adenosyl_L_homocysteine + H2O -> Adenine + S_Ribosyl_L_homocysteine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant Y107F]

H2O + S_Adenosyl_L_homocysteine -> S_Ribosyl_L_homocysteine + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant F105A]

H2O + S_Adenosyl_L_homocysteine -> Adenine + S_Ribosyl_L_homocysteine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22
uniprot:P0AF12 [adenosylhomocysteine nucleosidase(Enzyme) mutant S76A]

H2O + S_Adenosyl_L_homocysteine -> S_Ribosyl_L_homocysteine + Adenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.35 U/ml grade III buttermilk xanthine oxidase, 1 mM 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
PH: 7
Temperature: 22

Pathways

pathway id name
BioCyc:META_PWY-7371 1,4-dihydroxy-6-naphthoate biosynthesis II
BioCyc:META_PWY-6154 autoinducer AI-2 biosynthesis II (Vibrio)
BioCyc:META_PWY0-1391 S-methyl-5'-thioadenosine degradation IV
BioCyc:META_PWY-7992 superpathway of menaquinol-8 biosynthesis III
BioCyc:META_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:ECO_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:VCHO_PWY0-1391 S-methyl-5'-thioadenosine degradation IV
BioCyc:MTBCDC1551_PWY0-1391 S-methyl-5'-thioadenosine degradation IV
BioCyc:HPY_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:ECOL199310_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:ECOL199310_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:BSUB_PWY0-1391 S-methyl-5'-thioadenosine degradation IV
BioCyc:META_PWY-7528 L-methionine salvage cycle I (bacteria and plants)
BioCyc:ARA_PWY-6754 S-methyl-5'-thioadenosine degradation I
BioCyc:ARA_PWY-7528 L-methionine salvage cycle I (bacteria and plants)
BioCyc:MTBH37RV_PWY0-1391 S-methyl-5'-thioadenosine degradation IV
BioCyc:MTBCDC1551_PWY-6756 S-methyl-5'-thioadenosine degradation II
BioCyc:HPY_PWY0-1391 S-methyl-5'-thioadenosine degradation IV
BioCyc:BSUB_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:ECOL316407_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:GCF_000013425_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:META_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:META_PWY-6754 S-methyl-5'-thioadenosine degradation I
BioCyc:META_PWY-I9 L-cysteine biosynthesis VI (from L-methionine)
BioCyc:ECO_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:MTBH37RV_PWY-I9 L-cysteine biosynthesis VI (from L-methionine)
BioCyc:HPY_PWY-7371 1,4-dihydroxy-6-naphthoate biosynthesis II
BioCyc:HPY_PWY-7373 superpathway of demethylmenaquinol-6 biosynthesis II
BioCyc:HPY_PWY-I9 L-cysteine biosynthesis VI (from L-methionine)
BioCyc:ECOL199310_PWY0-1391 S-methyl-5'-thioadenosine degradation IV
BioCyc:BSUB_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:BSUB_PWY-6754 S-methyl-5'-thioadenosine degradation I
BioCyc:GCF_000013425_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:META_PWY-7373 superpathway of demethylmenaquinol-6 biosynthesis II
BioCyc:ECO_PWY0-1391 S-methyl-5'-thioadenosine degradation IV
BioCyc:ARA_PWY-6756 S-methyl-5'-thioadenosine degradation II
BioCyc:ARA_PWY-7270 L-methionine salvage cycle II (plants)
BioCyc:VCHO_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:VCHO_PWY-6154 autoinducer AI-2 biosynthesis II (Vibrio)
BioCyc:VCHO_PWY-I9 L-cysteine biosynthesis VI (from L-methionine)
BioCyc:GCF_000013425_PWY0-1391 S-methyl-5'-thioadenosine degradation IV
PathBank:SMP0000856 Quorum Sensing
PathBank:SMP0000857 S-Adenosyl-L-Methionine Biosynthesis
PathBank:SMP0002092 S-Adenosyl-L-Methionine Cycle
PathBank:SMP0002097 Spermidine Biosynthesis and Metabolism
PlantCyc:ARA_PWY-6756 S-methyl-5'-thioadenosine degradation II
PlantCyc:ARA_PWY-7270 L-methionine salvage cycle II (plants)
PlantCyc:ARA_PWY-6754 S-methyl-5'-thioadenosine degradation I
PlantCyc:ARA_PWY-7528 L-methionine salvage cycle I (bacteria and plants)