EC: 2.7.1.17

xylulokinase (ATP:D-xylulose 5-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q8X167 [xylulokinase(Enzyme) wildtype]

ATP + D_Xylulose -> ADP + D_Xylulose_5_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 1 U/ml lactate dehydrogenase, 3 U/ml pyruvate kinase
PH: 7.8
Temperature: 30
uniprot:Q8X167 [xylulokinase(Enzyme) wildtype]

ATP + D_Xylulose -> ADP + D_Xylulose_5_phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 1 U/ml lactate dehydrogenase, 3 U/ml pyruvate kinase
PH: 7.8
Temperature: 30
uniprot:P51811 [xylulokinase(Enzyme) wildtype N-terminal His-tag]

D_Xylulose + ATP -> D_Xylulose_5_phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 150 mM NaCl, 250 mM NADH, 1.5 mM MgCl2, 1.8 U pyruvate kinase, 12 U lactate dehydrogenase, 2 mM phosphoenolpyruvate
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:ECO_XYLCAT-PWY D-xylose degradation I
BioCyc:MOUSE_LARABITOLUTIL-PWY xylitol degradation
BioCyc:META_LARABITOLUTIL-PWY xylitol degradation
BioCyc:META_PWY-5257 superpathway of pentose and pentitol degradation
BioCyc:YEAST_LARABITOLUTIL-PWY xylitol degradation
BioCyc:ECOL316407_XYLCAT-PWY xylose degradation I
BioCyc:BSUB_XYLCAT-PWY D-xylose degradation I
BioCyc:META_XYLCAT-PWY D-xylose degradation I
BioCyc:ARA_XYLCAT-PWY xylose degradation I
PathBank:SMP0002117 Xylose Degradation I
PlantCyc:ARA_XYLCAT-PWY D-xylose degradation I
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-5661270 Formation of xylulose-5-phosphate
Reactome:R-HSA-5661270 Formation of xylulose-5-phosphate
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-5661270 Formation of xylulose-5-phosphate
Reactome:R-SCE-71387 Carbohydrate metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-5661270 Formation of xylulose-5-phosphate
Reactome:R-BTA-71387 Carbohydrate metabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-5661270 Formation of xylulose-5-phosphate
Reactome:R-SPO-71387 Carbohydrate metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-RNO-71387 Carbohydrate metabolism
Reactome:R-SCE-5661270 Formation of xylulose-5-phosphate
PathBank:SMP0002345 Xylitol Degradation